Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,403	C→T	V50I (GTC→ATC)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,403	0	C	T	100.0%	17.9 / NA	9	V50I (GTC→ATC)	araD	L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/6);  total (3/6)

GTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCT  >  NC_000913/66210‑66591                                                                                                                                                                                                  |                                                                                                                                                                                             nttGCTGGATTCGACTGGCACGCCTGCGCCCAGATAGTGGCGTGGTGCGCGTGCGTGTGAACAATGCCGACAAAGGACGGGAATGCCTGATAGAGCAGGCGGTGAGTTGGCGTATCGGAGGAGGGGTTTTTCGCACCTTCAACCAATTCACCGGTTTCGATGCTAACAACGACCATATCGTCGGAGGTCATGATGATGCAATAGACGACGGCAGGTTTGATCACAAAGACGCCGCGCTCGAGATCCACggg                                                                                                                                     >  1:51665/2‑250 (MQ=255)                       ccTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCGTCAACCACTTCACCGGTTTCGAGGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGc                                                                                                               <  1:224965/251‑1 (MQ=255)                                 gagagtggcgtggcGCGATTGGGTGGGCACAATGCCGCCAATGGACTCGAATGCCGGATAGAGCAGGCGGTGAGTTGTCGTATCGGAGGAAGGCTTTTTCGCGCCTTCAACCACTTCTCCGGTTTCGATGCTAACCACGACCATATCGTCAGCTGTCATGATGCTTTAATTGACGCCTGAAGTTTGGATGACAAACACCCCTCGCTCGCCATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGt                                                                                                     <  1:271431/247‑1 (MQ=255)                                          gtggcgCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGGGAGTTGTCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGTTCATTATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGgc                                                                                                                                     <  1:144455/210‑1 (MQ=255)                                                                             acgggAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTg                                                         <  1:183184/249‑1 (MQ=255)                                                                                                          gTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCGCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAAGACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTg                            <  1:205704/251‑1 (MQ=255)                                                                                                                        ggaggGCTTTTTCGCACCTTCAACCANTTCACCNNTTNCGATGCTAACCACGACCATNTCNNNANNNGNCATGANGCNNTACTCGACGNCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGNCCCATGTGANNGTGCCCAGGTTATGTTTTGGCNGCGNNANNTNGGcncc                                                                         >  2:61915/1‑189 (MQ=255)                                                                                                                        ggaggGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGGTGGCCTCTAATAc                                                                   <  1:61915/198‑1 (MQ=255)                                                                                                                                     gcaCCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGCTGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGAAGCCGCGATCGAGATCAACgg                                                                                                                                      >  1:134316/3‑118 (MQ=255)                                                                                                                                     gNACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGGTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCt  >  1:215660/1‑250 (MQ=255)                                                                                                                                                                                                  |                                                                                                                                                                                             GTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCT  >  NC_000913/66210‑66591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑