| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 66,343 | T→C | T70A (ACG→GCG) | araD ← | L‑ribulose‑5‑phosphate 4‑epimerase AraD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 66,343 | 0 | T | C | 91.7% | 31.5 / NA | 12 | T70A (ACG→GCG) | araD | L‑ribulose‑5‑phosphate 4‑epimerase AraD |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (3/8); minor base A (1/0); total (4/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCT > NC_000913/66127‑66591 | cATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATAGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGACTGCGCCCAGATAGTGGCGTGGCGCGTGTGGGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGTAGTCGGTGGGTTGGCGGATCGGGGGAGGGGTTTTTCGACCCTTCAACCACTTCACCGGTTTCGATGCCAAcc > 1:254727/1‑251 (MQ=255) aTTTCTGCGTCGGTCATTTTGCGGGGGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATATAGCAGTCGGTGAGTTGGCTTATCGGAGGAGGGCTTGTTCGCACCTTCAACCACtt < 1:83302/217‑1 (MQ=255) gCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGGGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGTGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAgn < 1:172975/248‑2 (MQ=255) gCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGAGAGTGTGCACAATGCCGCCAATGGACGGGGATGCCTGATAGAGCAGGCGGGGGGTTGGCGTATCGGGGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTccg > 1:114732/1‑186 (MQ=255) nttGCTGGATTCGACTGGCACGCCTGCGCCCAGATAGTGGCGTGGTGCGCGTGCGTGTGAACAATGCCGACAAAGGACGGGAATGCCTGATAGAGCAGGCGGTGAGTTGGCGTATCGGAGGAGGGGTTTTTCGCACCTTCAACCAATTCACCGGTTTCGATGCTAACAACGACCATATCGTCGGAGGTCATGATGATGCAATAGACGACGGCAGGTTTGATCACAAAGACGCCGCGCTCGAGATCCACggg > 1:51665/2‑250 (MQ=255) ccTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCGTCAACCACTTCACCGGTTTCGAGGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGc < 1:224965/251‑1 (MQ=255) gagagtggcgtggcGCGATTGGGTGGGCACAATGCCGCCAATGGACTCGAATGCCGGATAGAGCAGGCGGTGAGTTGTCGTATCGGAGGAAGGCTTTTTCGCGCCTTCAACCACTTCTCCGGTTTCGATGCTAACCACGACCATATCGTCAGCTGTCATGATGCTTTAATTGACGCCTGAAGTTTGGATGACAAACACCCCTCGCTCGCCATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGt < 1:271431/247‑1 (MQ=255) gtggcgCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGGGAGTTGTCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGTTCATTATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGgc < 1:144455/210‑1 (MQ=255) acgggAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTg < 1:183184/249‑1 (MQ=255) gTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCGCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAAGACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTg < 1:205704/251‑1 (MQ=255) anaangGCTTNTTCGNACCTTCAACCACTTCNCCGGTTTCGATGCTAACCANNANCATATNGTCAg < 2:114732/63‑1 (MQ=255) ggaggGCTTTTTCGCACCTTCAACCANTTCACCNNTTNCGATGCTAACCACGACCATNTCNNNANNNGNCATGANGCNNTACTCGACGNCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGNCCCATGTGANNGTGCCCAGGTTATGTTTTGGCNGCGNNANNTNGGcncc > 2:61915/1‑189 (MQ=255) ggaggGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGGTGGCCTCTAATAc < 1:61915/198‑1 (MQ=255) gNACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGGTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCt > 1:215660/1‑250 (MQ=255) | CATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCT > NC_000913/66127‑66591 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |