Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,266	A→G	H95H (CAT→CAC)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,266	0	A	G	100.0%	25.6 / NA	12	H95H (CAT→CAC)	araD	L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/5);  total (7/5)

CGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGT  >  NC_000913/66055‑66492                                                                                                                                                                                                                    |                                                                                                                                                                                                                                   cGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGGGGCGCGAGGGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGAAAGagc                                                                                                                                                                                             >  1:73746/1‑251 (MQ=255)                                         ggTTTCTACGATGACGTTACCGGTTTCCCACTCATATCAATAGTTGATTTATGGGTCGGTCTTTTTGCGGGTGCAGGGAATGGTGACGTAGAATTAGTCGGCGTGGGTGGCGCCGGTTGCCGGAATCGACTGGCCCGCCTGCACCCAGATAGTGGCGTGGCGCGCGTGTGTGTGCAAAATGCAGCCAAttg                                                                                                                                                                                                                 >  1:260852/1‑189 (MQ=255)                                                                         cATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATAGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGACTGCGCCCAGATAGTGGCGTGGCGCGTGTGGGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGTAGTCGGTGGGTTGGCGGATCGGGGGAGGGGTTTTTCGACCCTTCAACCACTTCACCGGTTTCGATGCCAAcc                                                                                                                     >  1:254727/1‑251 (MQ=255)                                                                                       aTTTCTGCGTCGGTCATTTTGCGGGGGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATATAGCAGTCGGTGAGTTGGCTTATCGGAGGAGGGCTTGTTCGCACCTTCAACCACtt                                                                                                                                         <  1:83302/217‑1 (MQ=255)                                                                                             gCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGGGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGTGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAgn                                                                                                    <  1:172975/248‑2 (MQ=255)                                                                                                  ggTCATTTTGCGGGTGAAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAAt                                                                                                                                                                                                                            >  1:212432/1‑123 (MQ=255)                                                                                                     cATTTTGTGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCCCAATGCCGCCAATGGACGGGAATGCCTGATAGagc                                                                                                                                                                                             >  1:229716/1‑151 (MQ=255)                                                                                                                                                        gCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGAGAGTGTGCACAATGCCGCCAATGGACGGGGATGCCTGATAGAGCAGGCGGGGGGTTGGCGTATCGGGGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTccg                                                                                                     >  1:114732/1‑186 (MQ=255)                                                                                                                                                            nttGCTGGATTCGACTGGCACGCCTGCGCCCAGATAGTGGCGTGGTGCGCGTGCGTGTGAACAATGCCGACAAAGGACGGGAATGCCTGATAGAGCAGGCGGTGAGTTGGCGTATCGGAGGAGGGGTTTTTCGCACCTTCAACCAATTCACCGGTTTCGATGCTAACAACGACCATATCGTCGGAGGTCATGATGATGCAATAGACGACGGCAGGTTTGATCACAAAGACGCCGCGCTCGAGATCCACggg                                  >  1:51665/2‑250 (MQ=255)                                                                                                                                                                                  ccTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCGTCAACCACTTCACCGGTTTCGAGGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGc            <  1:224965/251‑1 (MQ=255)                                                                                                                                                                                            gagagtggcgtggcGCGATTGGGTGGGCACAATGCCGCCAATGGACTCGAATGCCGGATAGAGCAGGCGGTGAGTTGTCGTATCGGAGGAAGGCTTTTTCGCGCCTTCAACCACTTCTCCGGTTTCGATGCTAACCACGACCATATCGTCAGCTGTCATGATGCTTTAATTGACGCCTGAAGTTTGGATGACAAACACCCCTCGCTCGCCATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGt  <  1:271431/247‑1 (MQ=255)                                                                                                                                                                                                     gtggcgCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGGGAGTTGTCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGTTCATTATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGgc                                  <  1:144455/210‑1 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                                   CGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGT  >  NC_000913/66055‑66492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑