Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,245	G→A	T102T (ACC→ACT)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,245	0	G	A	100.0%	21.6 / NA	11	T102T (ACC→ACT)	araD	L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/3);  total (8/3)

GCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGC  >  NC_000913/65998‑66482                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              gCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCAGTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGTTGCCGTAGAAATAGTCGGCGTGGTTGGTGCCGGTTGCTGTAATCGACTGGCCCGCCTGCGCCCAGAtagtgn                                                                                                                                                                                                                                           <  1:24500/251‑2 (MQ=255)                                                          cGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGGGGCGCGAGGGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGAAAGagc                                                                                                                                                                                   >  1:73746/1‑251 (MQ=255)                                                                                                  ggTTTCTACGATGACGTTACCGGTTTCCCACTCATATCAATAGTTGATTTATGGGTCGGTCTTTTTGCGGGTGCAGGGAATGGTGACGTAGAATTAGTCGGCGTGGGTGGCGCCGGTTGCCGGAATCGACTGGCCCGCCTGCACCCAGATAGTGGCGTGGCGCGCGTGTGTGTGCAAAATGCAGCCAAttg                                                                                                                                                                                                       >  1:260852/1‑189 (MQ=255)                                                                                                                      cGGTTTACCACTCATATTCACAGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGAAGAACTAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCACGCCTGCGCCCAGATAGTNGCGNNNnncgnnnnnn                                                                                                                                                                                                                            >  2:172975/1‑144 (MQ=255)                                                                                                                                  cATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATAGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGACTGCGCCCAGATAGTGGCGTGGCGCGTGTGGGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGTAGTCGGTGGGTTGGCGGATCGGGGGAGGGGTTTTTCGACCCTTCAACCACTTCACCGGTTTCGATGCCAAcc                                                                                                           >  1:254727/1‑251 (MQ=255)                                                                                                                                                aTTTCTGCGTCGGTCATTTTGCGGGGGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATATAGCAGTCGGTGAGTTGGCTTATCGGAGGAGGGCTTGTTCGCACCTTCAACCACtt                                                                                                                               <  1:83302/217‑1 (MQ=255)                                                                                                                                                      gCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGGGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGTGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAgn                                                                                          <  1:172975/248‑2 (MQ=255)                                                                                                                                                           ggTCATTTTGCGGGTGAAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAAt                                                                                                                                                                                                                  >  1:212432/1‑123 (MQ=255)                                                                                                                                                              cATTTTGTGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCCCAATGCCGCCAATGGACGGGAATGCCTGATAGagc                                                                                                                                                                                   >  1:229716/1‑151 (MQ=255)                                                                                                                                                                                                                 gCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGAGAGTGTGCACAATGCCGCCAATGGACGGGGATGCCTGATAGAGCAGGCGGGGGGTTGGCGTATCGGGGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTccg                                                                                           >  1:114732/1‑186 (MQ=255)                                                                                                                                                                                                                     nttGCTGGATTCGACTGGCACGCCTGCGCCCAGATAGTGGCGTGGTGCGCGTGCGTGTGAACAATGCCGACAAAGGACGGGAATGCCTGATAGAGCAGGCGGTGAGTTGGCGTATCGGAGGAGGGGTTTTTCGCACCTTCAACCAATTCACCGGTTTCGATGCTAACAACGACCATATCGTCGGAGGTCATGATGATGCAATAGACGACGGCAGGTTTGATCACAAAGACGCCGCGCTCGAGATCCACggg                        >  1:51665/2‑250 (MQ=255)                                                                                                                                                                                                                                           ccTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCGTCAACCACTTCACCGGTTTCGAGGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGc  <  1:224965/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              GCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGC  >  NC_000913/65998‑66482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑