Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,134	G→A	G139G (GGC→GGT)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,134	0	G	A	100.0%	32.8 / NA	12	G139G (GGC→GGT)	araD	L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/7);  total (5/7)

ATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACC  >  NC_000913/65903‑66377                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    attcagcagCGTTTGCTGCATATCCGGTAACTGCGGCGCGAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCGTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGTACGCCGGGCATTTGCGCGGCATCGATACCCTGTTTTTCGAAGGTTTCGACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGt                                                                                                                                                                                                                                 <  1:270602/248‑1 (MQ=255)          cGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCg                                                                                                                                                                                                                        <  1:110959/251‑1 (MQ=255)                                                                        ccTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTGGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACt                                                                                                                                                          <  1:258716/251‑1 (MQ=255)                                                                           ctTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTGTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTGGAGGTCGTCGTCGGTCATTTTGCTGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTg                                                                                                                                                         <  1:276252/249‑1 (MQ=255)                                                                                                gCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCAGTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGTTGCCGTAGAAATAGTCGGCGTGGTTGGTGCCGGTTGCTGTAATCGACTGGCCCGCCTGCGCCCAGAtagtgn                                                                                                                                  <  1:24500/251‑2 (MQ=255)                                                                                                                                    ggCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTg                                                                                                                                                                   <  1:220350/183‑1 (MQ=255)                                                                                                                                                         cGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGGGGCGCGAGGGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGAAAGagc                                                                          >  1:73746/1‑251 (MQ=255)                                                                                                                                                                             tCGATACCCTTTTTTTCGAAGGTTTCTACGATGACGTTACCTGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGa                                                                                                                                                                                                              <  1:257828/99‑1 (MQ=255)                                                                                                                                                                                                 ggTTTCTACGATGACGTTACCGGTTTCCCACTCATATCAATAGTTGATTTATGGGTCGGTCTTTTTGCGGGTGCAGGGAATGGTGACGTAGAATTAGTCGGCGTGGGTGGCGCCGGTTGCCGGAATCGACTGGCCCGCCTGCACCCAGATAGTGGCGTGGCGCGCGTGTGTGTGCAAAATGCAGCCAAttg                                                                                              >  1:260852/1‑189 (MQ=255)                                                                                                                                                                                                       tACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGCTTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGc                                                                                                                                                                                                       >  1:127816/1‑80 (MQ=255)                                                                                                                                                                                                                     cGGTTTACCACTCATATTCACAGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGAAGAACTAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCACGCCTGCGCCCAGATAGTNGCGNNNnncgnnnnnn                                                                                                                   >  2:172975/1‑144 (MQ=255)                                                                                                                                                                                                                                 cATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATAGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGACTGCGCCCAGATAGTGGCGTGGCGCGTGTGGGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGTAGTCGGTGGGTTGGCGGATCGGGGGAGGGGTTTTTCGACCCTTCAACCACTTCACCGGTTTCGATGCCAAcc  >  1:254727/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    ATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACC  >  NC_000913/65903‑66377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑