Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,041	G→A	S170S (TCC→TCT)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,041	0	G	A	100.0%	22.0 / NA	10	S170S (TCC→TCT)	araD	L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/8);  total (2/8)

GCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGG  >  NC_000913/65871‑66249                                                                                                                                                                           |                                                                                                                                                                                                                 gCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCCGCGTTTGCTGCACATCCGGTAACTGCGGCGCTAACTGACGGCAGTCTATCCCCCTATCAGCGACCTCTTCCAGCAAGATGGGGTTATGCACCGCATCTTCGGCCTTTTTGCCC‑ATGCAACTGGGCCGTGAGAATGGACCCGGACGCACGGCATTTg                                                                                                                                                                                        >  1:159013/1‑196 (MQ=255)       gcgcCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCGTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAAt                                                                                                                                                                                                              <  1:119385/168‑1 (MQ=255)                                 attcagcagCGTTTGCTGCATATCCGGTAACTGCGGCGCGAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCGTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGTACGCCGGGCATTTGCGCGGCATCGATACCCTGTTTTTCGAAGGTTTCGACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGt                                                                                                 <  1:270602/248‑1 (MQ=255)                                          cGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCg                                                                                        <  1:110959/251‑1 (MQ=255)                                                                                  cAGAATATACCCATATAAGCGACCTCTTCCAGCACGATGGAGTTATGCACCGAATATTAGGCATTTTTGCAC‑ATGAAAATGGGCCGTGAGAATGGACCAGGACGCCgg                                                                                                                                                                                               >  1:192504/1‑108 (MQ=255)                                                                                                    gCGACCTCTTCCAGCACGATGGCGTTATGCTCCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCTCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCAc                                                                                                                              <  1:133925/155‑1 (MQ=255)                                                                                                        ccTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTGGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACt                          <  1:258716/251‑1 (MQ=255)                                                                                                           ctTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTGTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTGGAGGTCGTCGTCGGTCATTTTGCTGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTg                         <  1:276252/249‑1 (MQ=255)                                                                                                                                gCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCAGTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGTTGCCGTAGAAATAGTCGGCGTGGTTGGTGCCGGTTGCTGTAATCGACTGGCCCGCCTGCGCCCAGAtagtgn  <  1:24500/251‑2 (MQ=255)                                                                                                                                                                    ggCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTg                                   <  1:220350/183‑1 (MQ=255)                                                                                                                                                                           |                                                                                                                                                                                                                 GCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGG  >  NC_000913/65871‑66249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑