Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,905	C→T	D216N (GAT→AAT)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,905	0	C	T	100.0%	22.4 / NA	11	D216N (GAT→AAT)	araD	L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/7);  total (4/7)

CTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTG  >  NC_000913/65661‑66067                                                                                                                                                                                                                                                     |                                                                                                                                                                   cnctTGCGGTGCAAGAGTAACCTGCAACGGCCAGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGGAATTCCATCAAAAAACCAGGATTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGGGTTTATTcagc                                                                                                                                                                >  1:215201/1‑249 (MQ=255)   ctTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTcagcag                                                                                                                                                              <  1:242628/249‑1 (MQ=255)           gCAAGCGTAACCTGCAAGGGCCTGTTGGCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCAGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTtgctgc                                                                                                                                                    <  1:277594/251‑1 (MQ=255)                                                                     ctggCGGGGTGTACCGCCCGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTTATTTTAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAACGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAgn                                                                                                                  <  1:163896/224‑2 (MQ=255)                                                                                                                                          aaaCCAGGCGTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCatat                                                                                                                                                <  1:241534/128‑1 (MQ=255)                                                                                                                                                ggCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCCGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACctct                                                                                           >  1:262473/1‑175 (MQ=255)                                                                                                                                                                                    gggtttAGACAGGCATTACTGCCCGTAATATGGCTTCGCGCCATGCTTACTCAGATAGTGTTTATTCAGCAGCGTTTGCTTCATATCCGGTAGCTGCGGCGCTAGCGTACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCAc                                                                                   <  1:46432/144‑1 (MQ=255)                                                                                                                                                                                     gTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGGTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCTACCTCTTCCAGCACGATGGCGTTATgn                                                                      <  1:26470/159‑2 (MQ=255)                                                                                                                                                                                                                   gCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCCGCGTTTGCTGCACATCCGGTAACTGCGGCGCTAACTGACGGCAGTCTATCCCCCTATCAGCGACCTCTTCCAGCAAGATGGGGTTATGCACCGCATCTTCGGCCTTTTTGCCC‑ATGCAACTGGGCCGTGAGAATGGACCCGGACGCACGGCATTTg  >  1:159013/1‑196 (MQ=255)                                                                                                                                                                                                                         gcgcCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCGTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAAt                        <  1:119385/168‑1 (MQ=255)                                                                                                                                                                                                                                               gtTTANTCAGCNGNNTTTGCTGCATCTCCGGTAANTGCGGCGCTAACTGANGGCANAATATNCCNa                                                                                                         >  2:110959/1‑66 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                   CTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTG  >  NC_000913/65661‑66067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑