Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,811	G→T	intergenic (‑31/+44)	polB ← / ← araD	DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,811	0	G	T	100.0%	21.3 / NA	11	intergenic (‑31/+44)	polB/araD	DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (5/6);  total (5/6)

CGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCT  >  NC_000913/65574‑65978                                                                                                                                                                                                                                              |                                                                                                                                                                        cGCCAGCGGTGCCAGCCGAACGCCTTGTTCACCCTGAAAAATATGCTGGGCGCGGGGAACCGGATGGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCTTAACCTGCAACGCCCCGTTGTCCGTCTCCAGCCAGAAGAAGACTTCTGTCCCTTGCGGGTTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCTCGCTGTAATTCCATCAAAAAACCAGGCTTTATTATAGCCTGGttt                                                                                                                                                            <  1:20802/251‑1 (MQ=255)                       ccTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATCAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCCGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCCGGGTCGGGTTAAGATTAAACCTGCCCGCTCCCCGCTGTAATTCCATCACAAAACCCAGCTTGATTATAGCCTATTTTCGTTTGATTGGCTGTTGttttt                                                                                                                                      >  1:112010/1‑250 (MQ=255)                                                  gcgcGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAAACTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGGGGTTTTATACAGTCATTACTGCCCGTAATATGcc                                                                                                           >  1:211680/1‑251 (MQ=255)                                                                                        cnctTGCGGTGCAAGAGTAACCTGCAACGGCCAGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGGAATTCCATCAAAAAACCAGGATTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGGGTTTATTcagc                                                                       >  1:215201/1‑249 (MQ=255)                                                                                          ctTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTcagcag                                                                     <  1:242628/249‑1 (MQ=255)                                                                                                  gCAAGCGTAACCTGCAAGGGCCTGTTGGCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCAGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTtgctgc                                                           <  1:277594/251‑1 (MQ=255)                                                                                                                                                            ctggCGGGGTGTACCGCCCGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTTATTTTAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAACGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAgn                         <  1:163896/224‑2 (MQ=255)                                                                                                                                                            cTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGGTTTTAGCCTGGGTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTAATGCNCGTNNNNNGcnn                                                                                                          >  2:163896/1‑144 (MQ=255)                                                                                                                                                                cGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTg                                                                                                                        <  1:62508/128‑1 (MQ=255)                                                                                                                                                                                                                                 aaaCCAGGCGTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCatat                                                       <  1:241534/128‑1 (MQ=255)                                                                                                                                                                                                                                       ggCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCCGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACctct  >  1:262473/1‑175 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                        CGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCT  >  NC_000913/65574‑65978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑