Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,788	A→T	intergenic (‑8/+67)	polB ← / ← araD	DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,788	0	A	T	100.0%	18.5 / NA	9	intergenic (‑8/+67)	polB/araD	DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (4/5);  total (4/5)

CGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGA  >  NC_000913/65574‑65955                                                                                                                                                                                                                       |                                                                                                                                                                        cGCCAGCGGTGCCAGCCGAACGCCTTGTTCACCCTGAAAAATATGCTGGGCGCGGGGAACCGGATGGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCTTAACCTGCAACGCCCCGTTGTCCGTCTCCAGCCAGAAGAAGACTTCTGTCCCTTGCGGGTTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCTCGCTGTAATTCCATCAAAAAACCAGGCTTTATTATAGCCTGGttt                                                                                                                                     <  1:20802/251‑1 (MQ=255)                       ccTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATCAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCCGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCCGGGTCGGGTTAAGATTAAACCTGCCCGCTCCCCGCTGTAATTCCATCACAAAACCCAGCTTGATTATAGCCTATTTTCGTTTGATTGGCTGTTGttttt                                                                                                               >  1:112010/1‑250 (MQ=255)                                                  gcgcGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAAACTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGGGGTTTTATACAGTCATTACTGCCCGTAATATGcc                                                                                    >  1:211680/1‑251 (MQ=255)                                                                                        cnctTGCGGTGCAAGAGTAACCTGCAACGGCCAGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGGAATTCCATCAAAAAACCAGGATTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGGGTTTATTcagc                                                >  1:215201/1‑249 (MQ=255)                                                                                          ctTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTcagcag                                              <  1:242628/249‑1 (MQ=255)                                                                                                  gCAAGCGTAACCTGCAAGGGCCTGTTGGCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCAGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTtgctgc                                    <  1:277594/251‑1 (MQ=255)                                                                                                                                                            ctggCGGGGTGTACCGCCCGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTTATTTTAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAACGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAgn  <  1:163896/224‑2 (MQ=255)                                                                                                                                                            cTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGGTTTTAGCCTGGGTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTAATGCNCGTNNNNNGcnn                                                                                   >  2:163896/1‑144 (MQ=255)                                                                                                                                                                cGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTg                                                                                                 <  1:62508/128‑1 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                        CGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGA  >  NC_000913/65574‑65955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑