Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,594	A→C	F63V (TTT→GTT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,594	0	A	C	100.0%	14.9 / NA	9	F63V (TTT→GTT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (7/2);  total (7/2)

GGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTT  >  NC_000913/65350‑65824                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       ggttGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGTCCCACATCGGCCTCGTAGACGGTAACGCCTCCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCTGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTg                                                                                                                                                                                                                                  <  1:266230/248‑1 (MQ=255)        gTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACCCCGGTGAGGTGATAAACCGCTCCATCAGATCGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCAccc                                                                                                                                                                                                                           >  1:149847/1‑251 (MQ=255)                                                                     ngtgaTAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGATAAACACCACGGACTCtttgg                                                                                                                                                              >  1:164743/2‑248 (MQ=255)                                                                                                      cgggcGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTACTTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGGGCAAGCGTAACCTGCAACGGCCCGTTGTCCg                                                                                                                             >  1:261867/5‑251 (MQ=255)                                                                                                          cGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCCTCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGGAACCTGCCACGGCCCGTTGTc                                                                                                                               >  1:61480/1‑245 (MQ=255)                                                                                                            cacaTCGGCATCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCCCGGACTCTTGCGGGGCAAGCGTAACCTGCAACGGc                                                                                                                                       >  1:88247/1‑235 (MQ=255)                                                                                                                             cGGTAACGCCACCTTCACGCAGGCGCTTTTCGTACTTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGATAAACGCCACGGAATCTTTCGGGGCAAGCGTAACCTACAACGGCCCGGTGTTCGGCGCCAGCCAGGAGGAGGCTTCt                                                                                                      >  1:77966/1‑251 (MQ=255)                                                                                                                                                                                     cgACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATACTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGGGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAACCAGAAGGAGACTTCTGTCCCTTGCGGGGGGGCCCGCCAGGGGCGGGGTAAGATAAAAAATGCCTGCGCCa                                               >  1:92238/1‑250 (MQ=255)                                                                                                                                                                                                                                 cGCCAGCGGTGCCAGCCGAACGCCTTGTTCACCCTGAAAAATATGCTGGGCGCGGGGAACCGGATGGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCTTAACCTGCAACGCCCCGTTGTCCGTCTCCAGCCAGAAGAAGACTTCTGTCCCTTGCGGGTTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCTCGCTGTAATTCCATCAAAAAACCAGGCTTTATTATAGCCTGGttt  <  1:20802/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       GGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTT  >  NC_000913/65350‑65824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑