Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,454	A→G	R109R (CGT→CGC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,454	0	A	G	93.3%	32.9 / NA	15	R109R (CGT→CGC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (7/7);  minor base T (0/1);  total (7/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCG  >  NC_000913/65234‑65668                                                                                                                                                                                                                             |                                                                                                                                                                                                                       nccccAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGACGATGCAGTACAGCTCACCGTGGCGGGCGGTTTCAATATCAATAGAAACCCAATTGAGCGGGGGGCGATCGTCGGGGTGCGGGTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCCCCCCATCAGATAGCGTTTTGGAGGGCGCACATCTGACTCGTAGACGGTAACGccc                                                                                                                                                                                          >  1:57645/2‑250 (MQ=255)             aaaCGATGCGCTGCCCGCAGCCTTCCATGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAAGAGAACCCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGTTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAgg                                                                                                                                                                              <  1:108710/251‑1 (MQ=255)                                             aTGCAGTACAGCTTACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGGGGGCGCTCAACGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTGTTCGTAATTCATCAATTGGCGATGGgc                                                                                                                                              <  1:107317/251‑1 (MQ=255)                                                                                                                     ggttGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGTCCCACATCGGCCTCGTAGACGGTAACGCCTCCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCTGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTg                                                                      <  1:266230/248‑1 (MQ=255)                                                                                                                            gTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACCCCGGTGAGGTGATAAACCGCTCCATCAGATCGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCAccc                                                               >  1:149847/1‑251 (MQ=255)                                                                                                                                                  agcgCCATTGTGCATAGCTCCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACTCCACCGTCACGCAGGCGCGTTTCGTAATTCATCAATTGGCGATGGGCGCTACAGTAAAGGCCATACACCGGCTGGCGGTg                                                                                                             <  1:267990/180‑1 (MQ=255)                                                                                                                                                                   cACCCTCTACCCACACCGGTGAGGTGATAAACCGCTTCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGtn                                                                                                                                                                                                       <  1:27825/76‑2 (MQ=255)                                                                                                                                                                               ncacCGGTGAGGTGATAAACCGCTCCATCAGCTAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCAcc                                                                                                                                                                                        >  1:32038/2‑79 (MQ=255)                                                                                                                                                                                         ngtgaTAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGATAAACACCACGGACTCtttgg  >  1:164743/2‑248 (MQ=255)                                                                                                                                                                                            gaTAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGTCGGTGAAAATCCTTTa                                                                                                  <  1:19850/152‑1 (MQ=255)                                                                                                                                                                                                           naGATAGCGTTCTGTCNGGCGCACATCGGCCTCGTCGACGGTAACGCCTCCTTCACGCAGGNNCNNNTNGTAATtca                                                                                                                                                              <  2:197190/76‑1 (MQ=255)                                                                                                                                                                                                           naGATAGCGTTCGGGCNGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGNNCNNNTNGTAATtca                                                                                                                                                              <  2:197181/76‑1 (MQ=255)                                                                                                                                                                                                           cAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATtca                                                                                                                                                              >  1:197181/1‑77 (MQ=255)                                                                                                                                                                                                           cAGATAGCGTTCTGGAGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGATTTTGGTAAtaca                                                                                                                                                              >  1:197190/1‑74 (MQ=255)                                                                                                                                                                                                                         gCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAAt                                                                                                                                                         >  1:98124/1‑68 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                       GCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCG  >  NC_000913/65234‑65668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑