Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,381	T→C	T134A (ACT→GCT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,381	0	T	C	100.0%	26.7 / NA	12	T134A (ACT→GCT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/5);  total (7/5)

TGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCC  >  NC_000913/65156‑65607                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                   tGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTGGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATGGTGCATATCACCCTCGAc                                                                                                                                                                                                            <  1:235949/250‑1 (MQ=255)                                       tCCAGGTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGTCCCCAGCATATAAACGACGCGATGCCCGCAGCATTCCCGGCCGATGCAGTACAGCTAACCGTGGCGGGAGGCTTCAATATCAATAGAAACCCCCTTTAGGGGCGGACGATAGTCGGGATGCGGATTCAGGCCGGCCTTAACGATAACGCCATTGTGTATATCACCCTCGACCCACACCGGTGAGGTGGTAAACCGCTCCATCAGAAAGCg                                                                                                                                                                     >  1:130296/1‑251 (MQ=255)                                                       gcgcggaggCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGCTTAAATCTCAATAGAAACCCACATGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAAAGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCgggggcac                                                                                                                                                      >  1:82498/5‑244 (MQ=255)                                                                               nccccAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGACGATGCAGTACAGCTCACCGTGGCGGGCGGTTTCAATATCAATAGAAACCCAATTGAGCGGGGGGCGATCGTCGGGGTGCGGGTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCCCCCCATCAGATAGCGTTTTGGAGGGCGCACATCTGACTCGTAGACGGTAACGccc                                                                                                                             >  1:57645/2‑250 (MQ=255)                                                                                ccccAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAAAGATAGCGCCATTGTGc                                                                                                                                                                                                                          >  1:220284/1‑157 (MQ=255)                                                                                           aaaCGATGCGCTGCCCGCAGCCTTCCATGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAAGAGAACCCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGTTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAgg                                                                                                                 <  1:108710/251‑1 (MQ=255)                                                                                                                           aTGCAGTACAGCTTACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGGGGGCGCTCAACGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTGTTCGTAATTCATCAATTGGCGATGGgc                                                                                 <  1:107317/251‑1 (MQ=255)                                                                                                                                               gCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCggg                                                                                                                                                           >  1:7416/1‑156 (MQ=255)                                                                                                                                                                                                   ggttGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGTCCCACATCGGCCTCGTAGACGGTAACGCCTCCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCTGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTg         <  1:266230/248‑1 (MQ=255)                                                                                                                                                                                                          ntttCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCggcgagg                                                                                                                                                                                              >  1:169344/2‑63 (MQ=255)                                                                                                                                                                                                          gTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACCCCGGTGAGGTGATAAACCGCTCCATCAGATCGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCAccc  >  1:149847/1‑251 (MQ=255)                                                                                                                                                                                                          gTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCggtgagg                                                                                                                                                                                              <  2:169344/63‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                   TGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCC  >  NC_000913/65156‑65607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑