Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,316	T→A	I155I (ATA→ATT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,316	0	T	A	100.0%	19.1 / NA	9	I155I (ATA→ATT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (5/4);  total (5/4)

GTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGC  >  NC_000913/65154‑65528                                                                                                                                                                   |                                                                                                                                                                                                                     gTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGGGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGGCGGGATGCggn                                                                                                                                                                            <  1:68096/205‑2 (MQ=255)   tGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTGGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATGGTGCATATCACCCTCGAc                                                                                                                             <  1:235949/250‑1 (MQ=255)                                         tCCAGGTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGTCCCCAGCATATAAACGACGCGATGCCCGCAGCATTCCCGGCCGATGCAGTACAGCTAACCGTGGCGGGAGGCTTCAATATCAATAGAAACCCCCTTTAGGGGCGGACGATAGTCGGGATGCGGATTCAGGCCGGCCTTAACGATAACGCCATTGTGTATATCACCCTCGACCCACACCGGTGAGGTGGTAAACCGCTCCATCAGAAAGCg                                                                                      >  1:130296/1‑251 (MQ=255)                                                         gcgcggaggCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGCTTAAATCTCAATAGAAACCCACATGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAAAGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCgggggcac                                                                       >  1:82498/5‑244 (MQ=255)                                                                                 nccccAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGACGATGCAGTACAGCTCACCGTGGCGGGCGGTTTCAATATCAATAGAAACCCAATTGAGCGGGGGGCGATCGTCGGGGTGCGGGTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCCCCCCATCAGATAGCGTTTTGGAGGGCGCACATCTGACTCGTAGACGGTAACGccc                                              >  1:57645/2‑250 (MQ=255)                                                                                  ccccAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAAAGATAGCGCCATTGTGc                                                                                                                                           >  1:220284/1‑157 (MQ=255)                                                                                             aaaCGATGCGCTGCCCGCAGCCTTCCATGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAAGAGAACCCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGTTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAgg                                  <  1:108710/251‑1 (MQ=255)                                                                                                                             aTGCAGTACAGCTTACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGGGGGCGCTCAACGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTGTTCGTAATTCATCAATTGGCGATGGgc  <  1:107317/251‑1 (MQ=255)                                                                                                                                                 gCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCggg                                                                            >  1:7416/1‑156 (MQ=255)                                                                                                                                                                   |                                                                                                                                                                                                                     GTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGC  >  NC_000913/65154‑65528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑