Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,202	G→A	F193F (TTC→TTT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,202	0	G	A	100.0%	19.5 / NA	8	F193F (TTC→TTT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/3);  total (5/3)

CTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCG  >  NC_000913/64977‑65444                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   cTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCAATGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGAATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCAt                                                                                                                                                                                                                           >  1:47237/1‑251 (MQ=255)                                    cgcgCCACTCCAGCTCACTGTTATCTCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGcc                                                                                                                                                                                          <  1:207855/249‑1 (MQ=255)                                                                                               ncATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGCTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCAATATcaat                                                                                                                                >  1:24082/2‑245 (MQ=255)                                                                                                                 cGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCAt                                                                                                                                                                                                                           >  1:228605/1‑139 (MQ=255)                                                                                                                                 ccacGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGc                                                                                                                                                                                                             >  1:126399/1‑137 (MQ=255)                                                                                                                                                                                  gTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGGGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGGCGGGATGCggn                                                                                        <  1:68096/205‑2 (MQ=255)                                                                                                                                                                                    tGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTGGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATGGTGCATATCACCCTCGAc                                         <  1:235949/250‑1 (MQ=255)                                                                                                                                                                                                                          tCCAGGTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGTCCCCAGCATATAAACGACGCGATGCCCGCAGCATTCCCGGCCGATGCAGTACAGCTAACCGTGGCGGGAGGCTTCAATATCAATAGAAACCCCCTTTAGGGGCGGACGATAGTCGGGATGCGGATTCAGGCCGGCCTTAACGATAACGCCATTGTGTATATCACCCTCGACCCACACCGGTGAGGTGGTAAACCGCTCCATCAGAAAGCg  >  1:130296/1‑251 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   CTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCG  >  NC_000913/64977‑65444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑