Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,031	A→G	N250N (AAT→AAC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,031	0	A	G	100.0%	14.1 / NA	6	N250N (AAT→AAC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/3);  total (3/3)

GCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCC  >  NC_000913/64816‑65260                                                                                                                                                                                                                        |                                                                                                                                                                                                                                      gCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACg                                                                                                                                                                                                    <  1:111249/251‑1 (MQ=255)                                                                     cTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGCTACCGTCGATAATTAGCAGACCTTTAGTCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCACGCCACTCCCGCTAACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCCCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCACCCGATGATCACATCAGGAt                                                                                                                                >  1:229550/1‑251 (MQ=255)                                                                               acggTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGc                                                                                                                      >  1:229334/4‑251 (MQ=255)                                                                                                       aaaTTCCAGAACGCGGATTTCATCGCCTCGATACCGTCGATAATTAGCCTACCTTTAGCCTGGGCAAAAAAGACGCCTTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCTCCCCAGCCGCATCGTAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGTAGAGCGAACTTCACCACGTGCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAg                                                                                              <  1:254041/251‑1 (MQ=255)                                                                                                                                                                  cTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCAATGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGAATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCAt                                   >  1:47237/1‑251 (MQ=255)                                                                                                                                                                                                     cgcgCCACTCCAGCTCACTGTTATCTCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGcc  <  1:207855/249‑1 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                      GCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCC  >  NC_000913/64816‑65260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑