Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,755	G→A	I342I (ATC→ATT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,755	0	G	A	100.0%	13.8 / NA	7	I342I (ATC→ATT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

ACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGA‑ACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCA  >  NC_000913/64537‑64850                                                                                                                                                                                                                            |                                                                                                accgAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGA‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGATTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCGTTCAGGt                                                                  <  1:96202/247‑1 (MQ=255)                       gTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCCCTTCGCCGCGATTAGCAGCGACATAACCAGCGCGGTGCATACGAGGACACTAGAGATGACCAAAAGCCGACACCGA‑GCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGCAAATCTGCGTCACCAGCTCGCAATCTTTCAGGGTATAAGTCGCCAGCGCAGGttt                                            >  1:115821/1‑250 (MQ=255)                                                           gtggcACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGA‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCg        >  1:229110/3‑250 (MQ=255)                                                                aCTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCTCCGA‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCa  <  1:224170/251‑1 (MQ=255)                                                                aCTTCGCCGAGATTAGGCGCGACATAAACAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACAGATACC‑CCGTGGCGGTCTAACGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAACGACATGATTTCAGTTTTATGGAAAATCTGCGTCACAAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCAGACAATTTCGTCCATTCGATccc   >  1:199325/1‑250 (MQ=255)                                                                                                                                                           tctaCCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCg                <  1:119630/144‑1 (MQ=255)                                                                                                                                                                        cGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCCGTTTTATGGAAAATCTGCGTCACCAGCTCGCACTCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCa                      >  1:159363/1‑128 (MQ=255)                                                                                                                                                                                                                            |                                                                                                ACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGA‑ACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCA  >  NC_000913/64537‑64850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑