Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,749	G→A	H344H (CAC→CAT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,749	0	G	A	100.0%	16.8 / NA	8	H344H (CAC→CAT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/3);  total (5/3)

GACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGA‑ACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCA  >  NC_000913/64504‑64850                                                                                                                                                                                                                                                       |                                                                                                      gACGGGTAAAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGACGCGAATCCATTACGTAGCAGCCAGGGCTGGCGTGTGGACGAACTTCGCCGAGATTAGGAGAGACATAACCCGCGCGGTGCATACGCGGACAATTGAGATGACCAAACGCCGCCCCCGC‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTAACCGTCCCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATggc                                                                                                   >  1:272802/1‑249 (MQ=255)                                  accgAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGA‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGATTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCGTTCAGGt                                                                  <  1:96202/247‑1 (MQ=255)                                                        gTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCCCTTCGCCGCGATTAGCAGCGACATAACCAGCGCGGTGCATACGAGGACACTAGAGATGACCAAAAGCCGACACCGA‑GCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGCAAATCTGCGTCACCAGCTCGCAATCTTTCAGGGTATAAGTCGCCAGCGCAGGttt                                            >  1:115821/1‑250 (MQ=255)                                                                                            gtggcACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGA‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCg        >  1:229110/3‑250 (MQ=255)                                                                                                 aCTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCTCCGA‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCa  <  1:224170/251‑1 (MQ=255)                                                                                                 aCTTCGCCGAGATTAGGCGCGACATAAACAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACAGATACC‑CCGTGGCGGTCTAACGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAACGACATGATTTCAGTTTTATGGAAAATCTGCGTCACAAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCAGACAATTTCGTCCATTCGATccc   >  1:199325/1‑250 (MQ=255)                                                                                                                                                                                            tctaCCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCg                <  1:119630/144‑1 (MQ=255)                                                                                                                                                                                                         cGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCCGTTTTATGGAAAATCTGCGTCACCAGCTCGCACTCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCa                      >  1:159363/1‑128 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                      GACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGA‑ACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCA  >  NC_000913/64504‑64850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑