Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,716	C→T	R355R (CGG→CGA)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,716	0	C	T	90.0%	20.6 / ‑6.3	10	R355R (CGG→CGA)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (5/4);  total (6/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGA‑ACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCA  >  NC_000913/64481‑64850                                                                                                                                                                                                                                             |                                                                                                                                       caatcaGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGATATGCCCAAACGCCGCCACCGA‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGACGCCCGTTCGAGTAAAAAt                                                                                                                         >  1:14254/1‑251 (MQ=255)               cGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACTAGTACCGAATCATAAAGCTCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGAGGACCAAACGCCGCCGCCGA‑TC‑‑CCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGttt                                                                                                         <  1:137944/251‑1 (MQ=255)                        gACGGGTAAAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGACGCGAATCCATTACGTAGCAGCCAGGGCTGGCGTGTGGACGAACTTCGCCGAGATTAGGAGAGACATAACCCGCGCGGTGCATACGCGGACAATTGAGATGACCAAACGCCGCCCCCGC‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTAACCGTCCCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATggc                                                                                                   >  1:272802/1‑249 (MQ=255)                                                         accgAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGA‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGATTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCGTTCAGGt                                                                  <  1:96202/247‑1 (MQ=255)                                                                               gTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCCCTTCGCCGCGATTAGCAGCGACATAACCAGCGCGGTGCATACGAGGACACTAGAGATGACCAAAAGCCGACACCGA‑GCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGCAAATCTGCGTCACCAGCTCGCAATCTTTCAGGGTATAAGTCGCCAGCGCAGGttt                                            >  1:115821/1‑250 (MQ=255)                                                                                                                   gtggcACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGA‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCg        >  1:229110/3‑250 (MQ=255)                                                                                                                        aCTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCTCCGA‑TCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCa  <  1:224170/251‑1 (MQ=255)                                                                                                                        aCTTCGCCGAGATTAGGCGCGACATAAACAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACAGATACC‑CCGTGGCGGTCTAACGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAACGACATGATTTCAGTTTTATGGAAAATCTGCGTCACAAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCAGACAATTTCGTCCATTCGATccc   >  1:199325/1‑250 (MQ=255)                                                                                                                                                                                                                   tctaCCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCg                <  1:119630/144‑1 (MQ=255)                                                                                                                                                                                                                                cGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCCGTTTTATGGAAAATCTGCGTCACCAGCTCGCACTCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCa                      >  1:159363/1‑128 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                       CAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGA‑ACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCA  >  NC_000913/64481‑64850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑