Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,536	C→T	V415V (GTG→GTA)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,536	0	C	T	100.0%	31.1 / NA	12	V415V (GTG→GTA)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/5);  total (7/5)

TTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGA  >  NC_000913/64303‑64753                                                                                                                                                                                                                                          |                                                                                                                                                                                                                          ttCAGCGCCTGCGTGAGCGGTTTGTTACCCTGGCGTTTGGCTGCATCGCGCCCGTGCCAGATGTTAGTCACAAGCTCCGGCAGGCAATGGGTTTCTCGCGAAAACCAGGCATCGAGAAAACCTTCGGTACGGCGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGTTCAATTAGAAAGGTGCGGAGGATCGACGGGTACAGGCTTTTATAGTCCAGCGCCAGTACCGAATCATAAAGcc                                                                                                                                                                                                           <  1:277153/250‑1 (MQ=255)                           tcccTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGAACTGTGCTCTTGATCAGGCTGCGCCATGCCTTCCTCCAGCCCCACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAgccg                                                                                                                                                                                 <  1:280154/249‑1 (MQ=255)                                        gCTTCATCGCGCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTCCTGTGCTCTGGATCAGCCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTg                                                                                                                                                                   >  1:125643/1‑251 (MQ=255)                                        gCTTCATCGCGCCCGTGCCAGATGTTAGTCACAATCTCCGCCAGGCAATGTTTTTCTCGCGAGCACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCACGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTCTAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCCCGCAGCCGCCAGGGCTGGCGTg                                                                                                                                                                   >  1:150580/1‑251 (MQ=255)                                                        gCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATCGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCg                                                                                                                                                   >  1:76443/1‑251 (MQ=255)                                                                      acaATCTCCGGCAGGCAATGTTTTTCTCGCGCGAAACCGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATAGACGGGTACAGGCTTTTATAGTCCAGCACCAGTCCCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGAt                                                                                                                                               >  1:241101/1‑241 (MQ=255)                                                                                    gcaATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCACTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGcgcggg                                                                                                                       >  1:64642/1‑249 (MQ=255)                                                                                      aaTGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAAGCCATCACGGAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGcgcggtgn                                                                                                                     <  1:54616/251‑5 (MQ=255)                                                                                                                                                      cgcCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGGGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCGGGCGTGTGGCGGCACTTCGCCGAGATTATGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAggn                                                     <  1:28278/251‑2 (MQ=255)                                                                                                                                                                                   caatcaGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGATATGCCCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGACGCCCGTTCGAGTAAAAAt                        >  1:14254/1‑251 (MQ=255)                                                                                                                                                                                                 cGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACTAGTACCGAATCATAAAGCTCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGAGGACCAAACGCCGCCGCCGATC‑‑CCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGttt        <  1:137944/251‑1 (MQ=255)                                                                                                                                                                                                          gACGGGTAAAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGACGCGAATCCATTACGTAGCAGCCAGGGCTGGCGTGTGGACGAACTTCGCCGAGATTAGGAGAGACATAACCCGCGCGGTGCATACGCGGACAATTGAGATGACCAAACGCCGCCCCCGCTCCCCCGTGGCGGTCTACCGGCAGGCCGTTAACCGTCCCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATggc  >  1:272802/1‑249 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                          TTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGA  >  NC_000913/64303‑64753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑