Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT50.0% ‑5.4 / 15.0 16intergenic (+266/+376)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
Reads supporting (aligned to +/- strand):  ref base C (4/4);  new base T (6/2);  total (10/6)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.25e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATA  >  NC_000913/4295827‑4296258
                                                                                                                                                                                                                                         |                                                                                                                                                                                                      
gtgtgAATGCCTGATGCGTCGCTTGCCGCGTCTTATCAGGCCTTCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATTCCGCATCCGACATCAACGCTTGATGCGGCGCTTAACGCGTCTTATCAGGCCGACGCCAGACAGCGCGATTGTCTGATTTAGCGTGATTTTGTAGGTCGTATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTg                                                                                                                                                                                       <  1:186539/251‑1 (MQ=11)
            gATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGATCGGATAAGGCGTTTATGCCGCATCCGAAATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGCCGCTTGCCGCGTCt                                                                                                                                                                               >  1:203531/1‑247 (MQ=25)
                   cGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTCTGCCGCATCCGACATCAACGCCTGACGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTTTAGATCGGATAAGGAGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGATTGCCGCGTCTTATCCGGCCt                                                                                                                                                                     >  1:43535/1‑250 (MQ=11)
                         ccGCGTCTTATCTGTCCTACGCCAGACAGCGCAATATCCTGATTTAGCTTGATTTTGTAGGTCGGAGAAGGCGTTTCTGCCGCATCCTACTTCATCGCCTGATTCGACGCTTAACTCGTCTTATCAGGCCTTCGCCAGTCAGCTCAATAGCCTTATTTATCGTGATTTTGTTGGTCGGATAATGCGTTTACTCCTCGTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAGCTGGCCTACGCCAg                                                                                                                                                              <  1:35889/251‑1 (MQ=11)
                                 tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACTCTTTCCGCGTCTTATCAGGCCTACGTCAGACAnc                                                                                                                                                         <  1:215334/248‑1 (MQ=16)
                                                                            aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGCCATCAACGCCTGATGCGACGCTTAAAGCGTCTTCTCAGGCCTACGCCCGACAGCGCACTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg                                                                                                                                                                                              >  1:153395/1‑168 (MQ=11)
                                                                                                ngTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                                                                      >  1:56557/2‑172 (MQ=18)
                                                                                                                          ccTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCNTCt                                                                                                                                                                               >  2:172769/1‑137 (MQ=21)
                                                                                                                                                         ncTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGACGGCTAAGGCGTTTACGCCGCATCCGACATCAGCGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTAc                                                                                                                                                                   >  1:166463/2‑118 (MQ=14)
                                                                                                                                                          cTACGCCAGACAGCGCCATAGACTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                                                                                                                                 >  1:84707/1‑103 (MQ=11)
                                                                                                                                                                             aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcn                                                                                                                                                                                     <  1:171885/80‑2 (MQ=255)
                                                                                                                                                                             aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc                                                                                                                                                                                     >  2:171885/1‑80 (MQ=255)
                                                                                                                                                                                cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCANNCCTACgnn                                                                                                                                                                >  2:16654/1‑96 (MQ=255)
                                                                                                                                                                                cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGcc                               <  1:16654/227‑1 (MQ=14)
                                                                                                                                                                                                                           gcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTGATTTAGCGTGCTTTTGTAGGTCGGATACGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGCCGCTTCACGCGTCTTATCAGGCCTACGCCCGACAGCGCAATAGACTGATTTAGCGTGATTTTGTAGGTCGGATa  >  1:41245/1‑213 (MQ=11)
                                                                                                                                                                                                                                   cATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCg                                                                                                     <  1:90941/106‑1 (MQ=38)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                      
GTGTGAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATA  >  NC_000913/4295827‑4296258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑