Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,315,674	IS2 (+) +5 bp	coding (2187‑2191/2673 nt)	rcsD →	RcsD phosphotransferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2068941 =	NA (NA)	11 (0.940)	11/304	0.1	100%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	2315674 =	0 (0.000)					coding (2187/2673 nt)	rcsD	RcsD phosphotransferase
*	?	NC_000913	= 2070271	NA (NA)	16 (1.370)	14/304	0.0	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	= 2315678	0 (0.000)					coding (2191/2673 nt)	rcsD	RcsD phosphotransferase

TCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGTGACAGAATCGCCTCTGGGCGGAGATGAAAATGCGCAACTCCATGCCAGCGGCTATT  >  NC_000913/2315466‑2315918                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 tgaaaacgataaagcgtgactacatcagtatcatgcccaaaccagacgggttaacggcagcaaagaaccttgcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatatagggacaaatccaTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCtt                                                                                                                                                                                                            >  1:6994‑M2/209‑251 (MQ=255)                ngcTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGACGTTGCAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgat                                                                                                                                                                                               <  1:26874‑M2/248‑52 (MQ=255)                 gCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGtagactggccccctgaa                                                                                                                                                                                                                                 <  1:12232‑M2/214‑18 (MQ=255)                 gCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAATGTTTAGTGGATGATGTCTGCGTAATGGTGGATGTTACTGCGTCAGAAATTCTGAATATGGTCACTCTTCAGTTAGGAAATTTGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGtagactggccccctgaatctc                                                                                                                                                                                                                             >  1:274889‑M2/1‑197 (MQ=255)                  gactacatcagtatcatgcccaaaccagtcgggttaacggcagcaaagaaccttgcagaggcgttcgagcattataacgaatggcatccgcatagtgcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaTCAAGATTATGATATCTGTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGgcttgc                                                                                                                                                                                            <  1:139899‑M2/59‑1 (MQ=255)                           cgttactCTGTGCATATCAAAATGCTCGCAGCTGGCCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACAGGATGATGTCTGCGGAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATGTTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGAGTAATTAGTCAAGtagactggccccctgaatctccagacaac                                                                                                                                                                                                                     >  1:271385‑M2/4‑187 (MQ=255)                                             aaaaTGCTCGCAGCTGACCCGGAAGTTGAAGCGGAAGAAGAGCGTTTACTGGATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGtagactggccccctga                                                                                                                                                                                                                                  <  1:218555‑M2/185‑17 (MQ=255)                                                      gCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGCGTACTGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaac                                                                                                                                                         <  1:258736‑M2/249‑90 (MQ=255)                                                                        gAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGCGTAATTGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttaggn                                                                                                                                     >  1:168870‑M2/1‑142 (MQ=255)                                                                                                            gTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAACATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggcgtaccccacaggaaa                                                                                                   <  1:237221‑M2/249‑144 (MQ=255)                                                                                                                 ggtggATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCACCAGTTAGATAATTGGGGTGCCACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGtagactggccccctgcatctccagccaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggcgtaccacacaggaaaagat                                                                                               <  1:151649‑M2/248‑148 (MQ=255)                                                                                                                  gcgctgggttatcgctcgccacgggaatatctgcggcagcgggcttgtaatgggttaagtgataacagatgtctggaaatataggggcaaatccaTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGt                                                                                                                                                >  1:184529‑M2/96‑198 (MQ=255)                                                                                                                         gttatcgctcgccacgggaatatctgcggcaccgggcttgtcatgggttaagtaataacagatgactggaactctaggggcaaatccaTCAAGATTATGATCTCTTTTTCACGGATAATCCGTATAATCTTACTGCCTCTGGCTTGCTTTTAAGc                                                                                                                                                                                    >  1:177385‑M2/89‑155 (MQ=255)                                                                                                                           tatcgatcgccacgggaatatctgcggcagcgggcctataatgggttaagtgatgacagatgtctggaaatatagggacaaatacaTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAACTTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGAGGTATTACAATTAATTg                                                                                   >  1:101463‑M2/87‑250 (MQ=255)                                                                                                                                 gCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTTCAACCTGTATCACACCCGATGAAAGATGAATTAGGCAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtctt                                                                                                                                                                                         >  1:157474‑M2/1‑85 (MQ=255)                                                                                                                                   tccacgggaatatctgcggcagcgggcttgtaattggttaagtgataacatatgtctgtaaatataggggcaaatcgaTCAAGATTATGATATCTTTTTAACGGATAATCCGTc                                                                                                                                                                                                                   <  1:155635‑M2/36‑1 (MQ=255)                                                                                                                                          cGGAATATTGTCACTCGCCAGTTAGAAAATTTGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGtagtctggccccctgaatctccagacaaccaatatctcttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggcgtaccacacaggaaaagatcgcaattgttcagcagagctttgaaccg                                                                   >  1:22990‑M2/1‑76 (MQ=255)                                                                                                                                                             cttgtaatgggttaagttataacagatgtctggaaatataggggcaaatccaTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAgg                                                                                                        <  1:18183‑M2/143‑1 (MQ=255)                                                                                                                                                                tAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGtagactggccccctgaatctccagacaaacaatatcacttaaataagttataggcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggtgtaccacacatgaaaagatc                                                                                              >  1:211447‑M2/1‑54 (MQ=255)                                                                                                                                                                 aGAAAATTGGGGAGCAACCTGTATCACACCCGATTAAAGATTAATTAGTCAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggcgtaccacacaggaaatgatcgcaattgttcagcagag                                                                             >  1:250963‑M2/1‑53 (MQ=255)                                                                                                                                                                      ggttaagtgataacagatgtctggaaatataggggcaaatccaTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTACTCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACgg                                                                                                                                                               >  1:238124‑M2/44‑131 (MQ=255)                                                                                                                                                                                  ncagatgtctggaaatataggggcaaatccaTCAAGATTATGATATCTTTTTAANGGATAATCCNNCTNATCTTNCNNNCNNNGGNTTGCTTTTAAGCGATGATGNGTNNGGCGTANGGGAAATTGGGCCTGGTCAATTGTGc                                                                                                                                       <  2:60622‑M2/112‑1 (MQ=255)                                                                                                                                                                                  ccTGTATCACACCCGATGAAAGATTAATTAGTCAAGcagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagac                                                                                                                     <  1:232471‑M2/161‑126 (MQ=255)                                                                                                                                                                                  acagatgtctggcaatataggggcaaatccaTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTACTCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGc                                                                                                                                       >  1:60622‑M2/32‑143 (MQ=255)                                                                                                                                                                                          acacCCGATGAAAGATTAATTAGTCAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagtttttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggcgtaccacacaggaaaagatcgcaattgttcagcagagctttgaaccggggatgacggtctccctcgttgcccggcaacatggtgtagcagccagc                   >  1:230379‑M2/1‑28 (MQ=255)                                                                                                                                                                                                      aGATTAAGTAGTCAAGtagactggccccctgaatctccagacaaccaatatcacttaaataagtgatagtcttaatactagttgttagactagtcattggagaacagatgattgatgtcttagggccggagaaacgcagacggcgtaccacacaggaaaagatcgcaattgttcagcagagctttgaaccggggatgacgggctccctctgtgcccggcaacatggtgtagcagccagccagttatttctc       >  1:182475‑M2/1‑16 (MQ=255)                                                                                                                                                                                                           aatccaTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGCCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGCCATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACCATTAATTGACGTGCAACTGGCGCAGGAAGAGGTGACAGAATCGCCTCTGCGCGGAGATGAAAATGCGCAACTCCATGCCAGCGGCtatt  >  1:147569‑M2/7‑251 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 TCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGTGACAGAATCGCCTCTGGGCGGAGATGAAAATGCGCAACTCCATGCCAGCGGCTATT  >  NC_000913/2315466‑2315918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.