Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,293,279	IS5 (+) +4 bp	intergenic (‑357/‑245)	hns ← / → tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1293279 =	0 (0.000)	23 (1.970)	20/304	0.0	100%	intergenic (‑357/‑248)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1293282	0 (0.000)	11 (0.940)	9/304	0.2	100%	intergenic (‑360/‑245)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
	?	NC_000913	1299499 =	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

GGAGTTTATCATAATTCGTCAGTTTTTCAGCTCAACAGTATGCACAGAAGTGGGTATATGCGTTCTCCCTTACGAAGCCTTGCATAATCCTTCTGAGCTATCATTACAACTGCCTCGCTTGTTATAAGCGGGTAAATGACTGCTGGTAACTATTCACAATCTTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1293488‑1293279 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGC  >  NC_000913/2101749‑2101952                                                                                                                                                                                                                                                                                                                                                                                                                                GGAGTTTATCATAATTCGTCAGTTTTTCAGCTCAACAGTATGCACAGAAGTGGGTATATGCGTTCTCCCTTACGAAGCCTTGCATAATCCTTCTGAGCTATCATTACAACTGCCTCGCTTGTTATAAGCGGGTAAATGACTGCTGGTAACTATTCACAATCTTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTC                                                                                                                                                                     >  1:61317/1‑251       TATCATAATTCGTCAGTTTTTCAGCTCAACAGTATGCACAGAAGTGGGTATATGCGTTCTCCCTTAAGAAGCCTTGCATAATCCTTCTGAGCTATCATCACAACTGCCTCGCTTGTTATAAGCGGGTAAATGACTGCTGGTAACTATTCACAATCTTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAG                                                                                                                                                                   >  1:124785/1‑247                          TTCAGCTCAACAGTATGCACAGAAGTGGGTATATGCGTTCTCCCTTACGAAGCCGTGCATAATCCTTCTGAGCTATCATTACAACTGCCTCGCTTGTTATAAGCGGGTAAATGACTGCTGGTAACTATTCACAATCTTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTG                                                                                                                                             <  1:112820/250‑1                            CAGCTCAACAGTATGCACAGAAGTGGGTATATGCGTTCTCCCTTACGAAGCCTTGCATAATCCTTCTGAGCTATCATTACAACTGCCTCGCTTGTTATAAGCGGGTAAATGACTGCTGGTAACTATTCACAATCTTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAG                                                                                                                                           <  1:63775/250‑1                              GCTCAACAGTATGCACAGAAGTGGGTATATGCGTTCTCCCTTACGATGCCTTGCATAATCCTTCTGAGCTATCATTACAACTGCCTCGCTTGTTATAAGCGGGTAAATGACTGCTGGTAACGATTCACAATCTTTAACCTGTTGCTCAAGTAATAGCCCTCGGTGGACCTCCAGGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCG                                                                                                                                        <  1:108435/251‑1                               CTCAACAGTATGCACAGAAGTGGGTATATGCGTTCTCCCTTACGAAGCCTTGCATAATCCTTCTGAGCTATCATTACAACTGCCTCGCTTGTTATAAGCGGGTAAATGACTGCTGGTAACTATTCACAATCTTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGGGAAGGTGCGAATACGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCG                                                                                                                                        >  1:34982/1‑250                                                             CGTTCTCCCTTACGAAGCCTTGCATAATCCTTCTGAGCTATCATTACAACTGCCTCGCTTGTTATAAGCGGGTAAATGACTGCTGGTAACTATTCACAATCTTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGGGAAGGTGCGAATAAGCGGTGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGC                                                                                                         <  1:161564/251‑1                                                                  nCCNTTANGNNGNNTTGNATAATCCTTCTGAGCTATCATNNCAACTGCCTNGCTTGTTATAAGCGGGTAAATGACTGCTGGTAACTATTCACAATCTTTAACCTNTTGCGCAAGNNATNGCCCGNTNNNGNNNTCNAGGAGATAGGGAAGGTGCGNATNNGCGGGGNAATTCTTCTCGGCTGACTCAGTCATT                                                                                                                                                              <  2:61317/192‑1                                                                       TACGAAGCCTTGCATAATCCTTCTGAGCTATCATTACAACTGCCTCGCTTGTTATAAGCGGGTAAATGACTGCTGGTAACTATTCACAATCTTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCAGTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAG                                                                                                 <  1:265336/249‑1                                                                             nCCTTGCATAATCCTTCTGAGCTATCATTACAACTGCCTCGCTTGTTATAAGCGGGTAAATGACTGCTGGTAACTATTCACAATCTTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTC                                                                                         >  1:173389/2‑251                                                                             GCCTTGCATAATCCTTCTGAGCTATCATTACAACTGCCTCGCTTGTTATAAGCGGGTAAATGACTGCTGGTAACTATTCACACTCTTTAACCTGTTGCGCAAGTAATAGCCATCTGTTGACCTCCAGGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCCTGTTTGAGCC                                                                                                                                         >  1:187686/1‑203                                                                                  GCATAATCCTTCTGAGCTATCATTACAACTGCCTCGCTTGTTATAAGCGGGTAAATGACTGCTGGTAACTATTCACAATCTTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGGCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCG                                                                                     <  1:135005/250‑1                                                                                   CATAATCCTTCTGAGCTATCATTACAACTGCCTCTCTTGTTATAAGCGGGTAAATGACTGCTGGTATCTAGTCACAATCTTTAACCTTTTGCGCAAGTAATAGCCCTCTGTTGACCTCCATGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCT                                                                                                                                                                                  <  1:88215/156‑1                                                                                             nnGANNNNNCATNACAACTGCCTCGCTTGTTATAAGCGGGTAAATGACTGCTGGTAACTATTCACAATCTTTAACCTGTTGCGCATGTAATAGCCCTCTGTTGACCTCCAGGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCT                                                                                                                                                                                  <  2:167210/144‑1                                                                                                AGCTATCATTACAACTGCCTCGCTTGTTATAAGCGGGTAAATGACTGCTGGTAACTATTCACAATCTTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTT                                                                       >  1:134764/1‑250                                                                                                                                 CNGGTAAATGACTGCTGGTAACTATTCCCAATCTTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGT                                                                        >  1:216247/1‑216                                                                                                                                    GTAAATGACTGCTGGTAACTATTCACAATCTTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGT                                   >  1:232271/1‑250                                                                                                                                                 GGTAACTATTCACAATCTTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGC                     <  1:131398/251‑1                                                                                                                                                                                           CCTCTGTTGACCTCCAGGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGGTTGAGCCGATTTTTTCGCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTGTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGG       <  1:256023/223‑1                                                                                                                                                                                               TGTTGACCTCCAGGAGATAGGGAAGGGGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTAATTTCATTTCTTCATGTTTGAGCAGATTTTTTCTCCCGTAAATGCCTTGAATCAGCATATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGC  >  1:157225/1‑224                                                                                                                                                                                                        nnAGGNGATAGGGAAGGTGCGAATAAGNNGGGAAATTCNTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCNGTAAATGCCNNGANTCAGCNTNNNTNNNCCNTTTCTTCGCCATTTAAGGCNTTNNCCCCAGNTTTTAGTGAGATCTCTCCCACTGACG                                    <  2:58755/179‑1                                                                                                                                                                                                        CCAGGAGATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACG                                    >  1:58755/1‑181                                                                                                                                                                                                           GGAGATAGGGAAGGTGCGAATAAGCGTGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGGCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCG                                                                                     <  1:206527/129‑1                                                                                                                                                                                                                                                                                                                                                                                                                                GGAGTTTATCATAATTCGTCAGTTTTTCAGCTCAACAGTATGCACAGAAGTGGGTATATGCGTTCTCCCTTACGAAGCCTTGCATAATCCTTCTGAGCTATCATTACAACTGCCTCGCTTGTTATAAGCGGGTAAATGACTGCTGGTAACTATTCACAATCTTTAACCTGTTGCGCAAGTAATAGCCCTCTGTTGACCTCCAGGAGATAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1293488‑1293279 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGC  >  NC_000913/2101749‑2101952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.