Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,542,725 (T)6→5 intergenic (+92/‑390) fimE → / → fimA regulator for fimA/type 1 fimbriae major subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,542,7250T.100.0% 49.6 / NA 13intergenic (+92/‑390)fimE/fimAregulator for fimA/type 1 fimbriae major subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (7/6);  total (7/6)

CGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGC  >  NC_000913/4542544‑4542967
                                                                                                                                                                                     |                                                                                                                                                                                                                                                  
cGTTATACCGCCAGTAATGCTGCTCGTTTTGACGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCATAAATAAGTTACGTATTTTT‑CTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTg                                                                                                                                                                               >  1:77447/1‑250 (MQ=255)
                      ntCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCATAAATAAGTTACGTATTTTT‑CTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTAtc                                                                                                                                                                                >  1:80223/2‑226 (MQ=255)
                               ccGGATTATGGGAAAGAAATAATCTCATAAACGCATAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCATAACTAAGTTACGTATTTTT‑CTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGt                                                                                                                                               >  1:224797/1‑251 (MQ=255)
                                             agaaaTAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCATAAATAAGTTACGTATTTTT‑CTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTc                                                                                                                                 <  1:19185/251‑1 (MQ=255)
                                                           aaaCGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCATAAATAAGTTACGTATTTTG‑CTCAAGCATAAAAATAGTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACTTCCTCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGc                                                                                                                    <  1:178585/250‑1 (MQ=255)
                                                                  aaaTTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCATAAATAAGTTACGTATTTTT‑CTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCt                                                                                                            <  1:158740/251‑1 (MQ=255)
                                                                                                   ttGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCATAAATAAGTTACGTATTTTT‑CTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAat                                                                            >  1:153691/1‑250 (MQ=255)
                                                                                                                  aaaaaaCAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCATAAATAAGTTACGTATTTTT‑CTCAAGc                                                                                                                                                                                                                                             >  1:3033/1‑74 (MQ=255)
                                                                                                                  aaaaaaCAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCATAAATAAGTTACGTATTTTT‑CTCAAGc                                                                                                                                                                                                                                             <  2:3033/74‑1 (MQ=255)
                                                                                                                                  cAAGACAATTGGGGCCAAACTGTCCATATCATAAATAAGTTACGTATTGGT‑CTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTGTGATATGTAAAGTATTTCTAGTGTAAAGTAAGTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACg                                                                                                                     <  1:160564/178‑1 (MQ=255)
                                                                                                                                                  aaaCTGTCCATATCATAAATAAGTTACGTATTTTT‑CTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCCAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCcaacaga                             >  1:13792/1‑248 (MQ=255)
                                                                                                                                                                  aaataaGTTACGTATTTTT‑CTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTGTCTGTGGCTCGACGCATCTTCCTCATTCGTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAAGATTAAGCCAACAAATAAACTTAAAAAGTTTg            <  1:97107/251‑1 (MQ=255)
                                                                                                                                                                        gTTACGTATTTTT‑CTCAAGCATAAAAATATTAAAAAACGACCAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTCACGTTTCTGTGGCTCGACGCCTCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCcgcg      >  1:147711/1‑251 (MQ=255)
                                                                                                                                                                             cgtatttttCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTAGTTCTATTGTAAATTAATTTCACATCACCTCCGCTAGATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCGCTCCAAAAACCACCGCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATGAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGc  <  1:122226/247‑1 (MQ=255)
                                                                                                                                                                                     |                                                                                                                                                                                                                                                  
CGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGC  >  NC_000913/4542544‑4542967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑