Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,224,169	A→G	F234F (TTT→TTC)	pbpG ←	peptidoglycan DD‑endopeptidase PbpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,224,169	0	A	G	100.0%	51.3 / NA	18	F234F (TTT→TTC)	pbpG	peptidoglycan DD‑endopeptidase PbpG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/8);  total (10/8)

CCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTAAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTG  >  NC_000913/2223924‑2224397                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     ccATCCGAATTCACTTCATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCGCAACGCCGCGGCCGGCACAGGCATCACTTTGCCGGTTTCAATCCAGGTACGCAGGCGGCTGTCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGcc                                                                                                                                                                                                                                 <  1:25032/251‑1 (MQ=255)                                                                           gNTCAACACTGCTGCCGGCACAGGCATCCCTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACg                                                                                                                                                       >  1:350377/1‑251 (MQ=255)                                                                                                            aCCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCggg                                                                                                                      <  1:70221/251‑1 (MQ=255)                                                                                                             ccGGTTTCAATCCAGGTACGCAGGCGGCGGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGgt                                                                                                                     <  1:170159/251‑1 (MQ=255)                                                                                                                                        cTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTCCCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGACCGATCAACGGat                                                                                           >  1:439250/1‑250 (MQ=255)                                                                                                                                           gCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATCACCGTACGCATCACCAGACAATGGCCCGCCGAATTGGTGCAGCCGGTTTTGGTTAAc                                                                                                                                                                                                                    >  1:198098/1‑126 (MQ=255)                                                                                                                                                ngCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGc                                                                                  >  1:132017/2‑251 (MQ=255)                                                                                                                                                ggCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCAGCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGttt                                                                                                                                                                                                                            <  1:60300/113‑1 (MQ=255)                                                                                                                                                             tatTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACg                                                                                              <  1:155992/226‑1 (MQ=255)                                                                                                                                                              attggCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCan                                                                     <  1:268038/246‑2 (MQ=255)                                                                                                                                                                   ccAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTag                                                                                                                                              >  1:120260/1‑172 (MQ=255)                                                                                                                                                                                   cTACCAGCGCCACAGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCCTTGGTGAAGCCGGTTTTGGTTAATTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGAGGAACGGCCGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTCGTACTTAACTGCCCGATCCACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGtt                                               >  1:232385/1‑251 (MQ=255)                                                                                                                                                                                                                             cnAGACAATGGCACGCCGCATTGGTGACGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGtata                                                                                                                                                         >  1:348492/1‑103 (MQ=255)                                                                                                                                                                                                                              cAGACNNTGNNNCGNNGCATTGGTGAAGCCGGTTTTGGTNAACTGAATATTCCAGTTATCGCGATACACNANANGATTNNTATNGCGGAACGGCNGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACNTNACTGCCCGATCAACGGATATTg                                                                                       >  2:327415/1‑168 (MQ=255)                                                                                                                                                                                                                              cAGACAGTGGCCCGCCGCATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGATCGGCAGCGTATACGTCGGATTATATAAGTTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTg                                                                                       <  1:327415/168‑1 (MQ=255)                                                                                                                                                                                                                                gACAATGGCCCGCCGCATTGGTGAAGCCGCTTTTGGTTAACTGAATATTCCAGTTATCGCGCTACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCgtg  >  1:365136/1‑251 (MQ=255)                                                                                                                                                                                                                                            ncgcATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGgtagt                                                                                                                  >  1:267362/2‑127 (MQ=255)                                                                                                                                                                                                                                            ccgcATTGGTGAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGgtagt                                                                                                                  <  2:267362/127‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     CCATCCGAATTCACTTAATCGTTCTGTGCCGTCTGCCCCGCCGCCGCCATTTGGGCGGCTTTTTGTTTTTTATAGCTCAACGCTGCTGCCGGCACAGGCATCACTTTACCGGTTTCAATCCAGGTACGCAGGCGGCTGGCATCGGCAAAATGGGTATATTTGCCAAACGCGTCCATCACTACCAGCGCCACCGGTTTATTATTGATAACCGTACGCATCACCAGACAATGGCCCGCCGCATTGGTAAAGCCGGTTTTGGTTAACTGAATATTCCAGTTATCGCGATACACCAGATGATTAGTATTGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTG  >  NC_000913/2223924‑2224397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑