Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,040,433	C→A	A319D (GCC→GAC)	msrP →	protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,040,433	0	C	A	100.0%	41.0 / NA	17	A319D (GCC→GAC)	msrP	protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/6);  total (11/6)

TAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCG  >  NC_000913/2040218‑2040653                                                                                                                                                                                                                        |                                                                                                                                                                                                                             tAAAGGGATTCAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTTTATCGTGGCCTGGATTTg                                                                                                                                                                                            <  1:334039/250‑1 (MQ=255)                    gTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGCCGAGTACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCCTCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCg                                                                                                                                                                        >  1:168494/1‑251 (MQ=255)                                                                       gcgcCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAg                                                                                                                                                                <  1:164500/208‑1 (MQ=255)                                                                                     cGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTGACGCCGACCAGGTGGGATCGCTGTATCGTGGCCTGTATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTg                                                                                                                                    <  1:395293/222‑1 (MQ=255)                                                                                                            cGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAg                                                                                                                                                                                                                          >  1:31199/1‑113 (MQ=255)                                                                                                                tGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCTACCAGGTGGCATCGCTGTATCGTGGCCTGGATTGGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGtttn                                                                                                                                    <  1:40130/195‑2 (MQ=255)                                                                                                                         nccGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTgg                                                                   >  1:272341/2‑251 (MQ=255)                                                                                                                               cTGGTCACAGGCTAAAGAACGATTTATTGGTTCAGGCGGCATCCTCGATATACGGCGACAGCCCACGCTAATGTTTAATGGTTACGCCGAACAGGTGGGATCGCTGAATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTAACATGGCTGAAAGTTTGCCTGCATCTTTCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGGGGACTGGGTGCCg                                                             >  1:332413/1‑251 (MQ=255)                                                                                                                                                 aCGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGttt                                                                                                                                     >  1:295684/1‑161 (MQ=255)                                                                                                                                                           ggTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGGTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATGTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCt                                                                                                                                 <  1:262752/155‑1 (MQ=255)                                                                                                                                                                     gcATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTg                                                                                                                                                                                                       >  1:228621/1‑75 (MQ=255)                                                                                                                                                                      cATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGcc                                                                                                                                  >  1:120251/1‑143 (MQ=255)                                                                                                                                                                         ccTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTCCGCCGACCCGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGAGTCTGACAGCACAACAGGTGACATGGCTGAAAGTTTGCCTGCATc                                                                                                                            >  1:205942/1‑146 (MQ=255)                                                                                                                                                                                     gcgcCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGNNTCTGACNNc                                                                                                                                                               >  2:38155/1‑99 (MQ=255)                                                                                                                                                                                     gcgcCAGCCAACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGAATCTTg                                                                                                                         <  1:38155/137‑1 (MQ=255)                                                                                                                                                                                           nccaACGCTACTGTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCg  >  1:90402/2‑250 (MQ=255)                                                                                                                                                                                                       gTTTAATGGTTACGCCGACCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGAt                                                                                                                   >  1:181630/1‑125 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                             TAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACAGGTGACATGGCTGAAAGTTTGCCTGCATCTTGCCGGATTGTTGCCGTTTCTCTGGCTGGTCTGGGCGATCAATCACGGTGGACTGGGTGCCGATCCGGTGAAAGATATTCAGCATTTTACTGGTCGCACTGCACTGAAATTTTTGCTGGCG  >  NC_000913/2040218‑2040653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑