Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,641,002	T→C	intergenic (+27/+337)	ynfU → / ← cspB	putative zinc‑binding protein YnfU/cold shock‑like protein CspB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,641,002	0	T	C	100.0%	24.5 / NA	9	intergenic (+27/+337)	ynfU/cspB	putative zinc‑binding protein YnfU/cold shock‑like protein CspB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/2);  total (7/2)

TCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCTCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGAGATTGTGGTGCTTTTTGTGGGAGTCATCCACTTACGCACTTTGTTTTGCCATGCCAGCAGTTAGCTTCTGCTGTAAAACTATTCATGCAGCAAACCTGCACTTCACCACAATGGTTAGCATACTTTTCCTGATTAAGATTTTGCCAAATATGCTAGCCA  >  NC_000913/1640845‑1641218                                                                                                                                                              |                                                                                                                                                                                                                         cgtgtgctcttccgatcgcaaccTGCACCCAAGAGTCAGAACACAGGTTTTCAAGAGTACAAAAAGGTGCCCTTT‑GATCTGCCCTCATTGCAACAAAGTATTCCAGACAAATCTTAACGCTGTAGCCTGATTGATTTTATTAGGAACAAGTATTTGCTATATTTTAAGAATATAGTGAATGCAGATAATAAAAAACCCGCCTGAGCGGGTGTTAGATTGTGGTGCTTTTTGTGGGAGGCATCCACTTACg                                                                                                                             <  1:339210/228‑1 (MQ=255)                   cAAACNNCANNNAANNGTCAGCACACGGTTTTTCAAGAGNACAAAAAGGTGCCCTTT‑GATCTGCCCTCANTNCNACAANNAATNCAAGACACATNTTAGAGATATAGCCTGATTGATTTTATTGGTAGCAAGTATTTTCtat                                                                                                                                                                                                                       >  2:339210/1‑142 (MQ=255)                                          cacaGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCTCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTCTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGAGATTgtggt                                                                                                                                                        >  1:286781/1‑183 (MQ=255)                                                       nagTACAAAAAGGTGCCCTTTTGATCTGCCCTCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTCTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGAGATTGTGGTGCTTTTTGTGGGAGTCATcc                                                                                                                                    >  1:111105/2‑190 (MQ=255)                                                         gTACAAAAAGGTGCCCTTTTGATCTGCCCTCATTGCAACAAAGTATTCCAGACACATCTTAACGCTGTAGCCTGATTGATTTTATTCGTAACAAGTATTTTCTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGAGATTGTGGTGCTTTTTGTGGGAGTCATCCACTTACGCACTTTGTTTTGCCATGCCAGCAGTTa                                                                                                  >  1:206587/1‑222 (MQ=255)                                                                   gTGCCCTTTTGATCTGCCCTCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTCTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGAGAt                                                                                                                                                              >  1:33913/1‑152 (MQ=255)                                                                      nccTTTTGATCTGCCCTCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTCTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGAGATTGTGGTGCTTTTTGTgg                                                                                                                                             >  1:114898/2‑166 (MQ=255)                                                                                                                             aGCCTGATTTATTTTATTAGTAACAAGTATTTTCTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGAGATTGTGGTGCTTTTTGTGGGAGTCATCCACTTACGCACTTTGTTTTGCCATGCCAGCAGTTAGCTTCGGCTGTAAAACTATTCATGCAGCAAACCTGCACTTCCCCACAATGGTTAGCATACTTTTCCTGATTAAGATTTTGCCAAATATGCTAGCCa  <  1:372274/250‑1 (MQ=255)                                                                                                                                                      aGTATTTTCTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGAGATTGTGGTGCTTTTTGTGGGAGTCATCCTCTTACGCACTTTGTTTtgccatgc                                                                                                           >  1:2803/1‑120 (MQ=255)                                                                                                                                                              |                                                                                                                                                                                                                         TCGTTAAATGTTCCTGCCCAAACTGCACCCAAGAGTCAGAACACAGTTTTTCAAGAGTACAAAAAGGTGCCCTTTTGATCTGCCCTCATTGCAACAAAGTATTCCAGACAAATCTTAAAGCTGTAGCCTGATTGATTTTATTAGTAACAAGTATTTTTTATATTTTAATAATATATTTAAAGCAGATAATAAAAAACCCGCCTGAGCGGGTTTGAGATTGTGGTGCTTTTTGTGGGAGTCATCCACTTACGCACTTTGTTTTGCCATGCCAGCAGTTAGCTTCTGCTGTAAAACTATTCATGCAGCAAACCTGCACTTCACCACAATGGTTAGCATACTTTTCCTGATTAAGATTTTGCCAAATATGCTAGCCA  >  NC_000913/1640845‑1641218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑