Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,600,234 C→T intergenic (‑25/+54) lsrK ← / ← lsrR autoinducer‑2 kinase/DNA‑binding transcriptional repressor LsrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,600,2340CT95.2% 54.2 / NA 21intergenic (‑25/+54)lsrK/lsrRautoinducer‑2 kinase/DNA‑binding transcriptional repressor LsrR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (7/13);  minor base G (0/1);  total (7/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGTACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGCCATCAGGTAGTACTTTGATTCTGAAAGGGTAAAGA‑GTCGAGCCATTAGATTATCCTCGGCTTATGCGTTCTGTCTGTTCCTCTATACGTTCTCCATCATTCCCGGTAATAAGGTCATGCAAATTTAACTACGTAAAATCGCCGCTGCTGTGTCCTGATCGGTAACCAGTGCGTTGATATAACCGCCTTTCATTGCAGCGGCAATTGCTTCGGCTTTATTTTCTCCCCCTGCCACGCCAACCCGGACGGGTATGGTCTTCAGCGCGCTTAAAGGTAAGCCAATCAGTTCGTTATG  >  NC_000913/1600017‑1600458
                                                                                                                                                                                                                          |                                                                                                                                                                                                                                
gttgccagttTTTGTTGATATCAAATCCCATAGAACCAGGAACGTCCGGTACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGTCATCAGGTAGTACTTTGATTCTGAAAGGGTAAAGA‑GTCGAGCCATTAGATTATCCTCGGCTTATGCGTTTTGTCTGTTCCTCTATACGTTCTCCATCATTccn                                                                                                                                                                                                 <  1:274084/251‑2 (MQ=255)
                                                     cccAGATGACGCCTCTCCGCGTGTCCCCCTGCTATTTGATTGCCTTCCAGGTCAAATATCCCAGCCCGAATACGTCCGGGGCCTGCATCCAGCGCCATGAGTTAGTACGTTGATTCGGAACGGGTAAAGA‑GTCTAGCCATTAGATTATCCTCGGCTTAGGCGTTTTGTCTGTTCCTCTATACGTTCTCCAGCATTCCCGGTAATAAGGTCATGCAAATTTAACTTCGTAAAATCGCCGCTGCTGTGTcc                                                                                                                                              <  1:78394/248‑1 (MQ=255)
                                                               gccACTGCGCCTGTCCCACTGCTAGTTGAGTGCCTTCCAGGTCGAATATCGCAGCCCGAATACTTCTGGTGCCTGCGTCCAGCGGAATCAGGTAGTACTTTGATTGTGAAAGGTTAAAGATGT‑GAGCCATTAGATTATCCTCGGCTTACGTGTTGTGTCTGTTCCTCTATACGTGCGCCATCATTTCCGGTAATAAGGTCTTGCAAATTTAACTACGGAAAATCGCCGCTGCTGTGTCCTGATCGGTAAcc                                                                                                                                  <  1:218663/251‑1 (MQ=255)
                                                               gccACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCATGCATCCAGCGCCATCAGGTAGTACTTGGATTCTGAAAGGGTAAAGA‑GTCGAGCCATTAGATTATCCTCGGCTTATGCGATTTGTCTGTTCCTCTATACGTTCTCCATCATTCCCGGTAATAAGGTCATGCAAATTTAACTACGTAAAATCGCCGCTGCTGTGTCCTGATCGGTAAcc                                                                                                                                  <  1:304623/251‑1 (MQ=255)
                                                               cccACTCCCCCTGTCCCACTTCTATTTGATTGCCTGCCAGGTCGAATATCCCAGCCCGAATACTTCCTGTGCATGCATCCAGCGCCATCAGTTAGTACTTTGATTCTGAAATGGTAAAGA‑GTCGAGCCATTAGATTATCCTCGGCTTATGCGTTTTGTCTGTTCCTCTATACGTTCTCCATCATTCCCGGTAATAAGGTCATGCAAATTTAACTACGTAAAATCGCCGCTGCTGTGTCCTGATCGGTAAcc                                                                                                                                  <  1:304616/250‑1 (MQ=255)
                                                                                      aTTTGATTGACTTCCAGGTCGAATATNACAGCCNNAANACTTCCGGTGCCTGCATCCNGCNNNANNNGNTAGTANTTNNATTCTGAAANGGTAAAGA‑GTCGAGCCATTAGATTATCCTCGGCTTATGCGTTTTGTCTGTTCCNCTATACGTTNNCCATCATTCCCGGTAATAAGGNCATNNANNTNTAANt                                                                                                                                                                       >  2:98973/1‑191 (MQ=255)
                                                                                               ccTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGCCATCAGGTAGTACTTTGATTCTGAACGGGTAAAGA‑GTCGAGCCATTAGATTATCCTCGGCTTATGCGTTTTGTCTGTTCCTCTATACGTTCTCCATCATTCCCGGTAATAAGGTCATGCAAATTTAACTACGTAAAATCGCCGCTGCTGTGTCCTGATCGGTAACCAGTGCGTTGATATAACCGCCTTTCATTGCAGc                                                                                                  >  1:310710/1‑251 (MQ=255)
                                                                                                 ttCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGCCATCAGGTAGTACTTTGATTCGGAAAGGGTAAAGA‑GTCGAGCCATTAGATTATCCTCGGCGTATGCGTTTTGTCTGTTCCTCTATACGTTCTCCATCATTCCCGGTAATAAgg                                                                                                                                                                                       <  1:235458/164‑1 (MQ=255)
                                                                                                                cccaGCCCGAATTCTACCGGTGCCTGCATCCAGCGCCATCAGGTAGTACTTTGATTCTGAAAGGGTAAAGA‑TTCGAGCCATTAGATTATCCTCGGCTTATGCGTTTTGTCTGTTCCTCTATACGTTCTCCATCATTCCCGGTAATAAGGTCATGCAAATTTAACTACGTAAAATCGCCGCTGCTGTGTCCTGATCGGTAACCAGTGCGTTGATATAACCGCCTTTCATTGTAGCGGCAATTGTTTCGGCtt                                                                                 <  1:98973/249‑1 (MQ=255)
                                                                                                                             cTTCCGGTGCCTGCATCCAGCGCCATCAGGTAGTACTTTGATTCTGAAAGGGTAAAGA‑GTCGAGCCATTAGATTATCCTCGGCTTATGTGTTTTGTCTGTTCCTCTATACGTTCTCCATCATTCCCGGTAATAAGGTCATGCAAATTTAACTACGTAAAATCGCCGCTGCTGTGTcc                                                                                                                                              >  1:26213/1‑177 (MQ=255)
                                                                                                                             cTTCCGGTGCCGGCATCCAGCGCCATCAGGTAGTACTTTGATTCTGAAAGGAAAAAGA‑GTCGAGCCATAAGATTATCCTCGGCTTCTCCGTGTTGTCTGTTCCGCTATACGTTCTCCATCATTCCCGGTAATAAGGTCATGCAACTTTTACTACGTAAAATCGCCGctgctg                                                                                                                                                   >  1:233662/1‑172 (MQ=255)
                                                                                                                                      ccTGCATCCAGCGCCATCAGGTAGTACTTTGATTCTGAAAGGGTAAAGA‑GTCGAGCCATTAGATTATCCTCGGCTTATGCGTTTTGTCTGTTCCTCTATACGTTCTCCATCATTCCCGGTAATAAGGTCATGCAAATTTAACTACGTAAAATcg                                                                                                                                                            <  1:345912/154‑1 (MQ=255)
                                                                                                                                       cTGCATCCAGCGCCATCAGGTAGTACTTTGATTCTGAAAGGGTAAAGA‑GTCGAGCCATTAGATTATCCTCGGCTTATGCGTTTTGTCTGTTCCTCTATACGTTCTCCATCATTCCCGGTAATAAGGTCATGCCAATTTAACTACGTAAAATCGCCGCTGCTGTGTCCTGATCGGTAACCAGTGCGTTGATATAAcc                                                                                                                 >  1:425212/1‑196 (MQ=255)
                                                                                                                                                                     aTTCTGAAAGTGTAAATG‑GTCGAGCCATGAGATGATCCTCGGCTTATGCGTTTTGTCTGTTCTTCTATACGTTCTCCATCATTCCCGATAATAAGGTCAGGCAAATTTAACTACGTAAACTCGTCGCTGCTGTGGCCTAATCGGTATTCAGTTCTTTGATATAACCGCCTTTCAGTTCAGCGGCAATTGCTTCGGCTTTATTTTCTCCCCCTGCCACGCCAACCCGGACGGGTATGTTCTTCAGCGCGCt                             <  1:331827/250‑1 (MQ=255)
                                                                                                                                                                        cTGAAAGGGGAAAGA‑GTCGAGCCATTAGATTATCCTCGGCTTATGCGTTTTGTCTGTTCCTCTATACGTtc                                                                                                                                                                                                             <  1:228363/71‑1 (MQ=255)
                                                                                                                                                                         tGAAAGGGTAAAGA‑GTCGAGCCATTAGATTATCCTCGGCTTATGCGTTTTGTCTGTTCCTCTATACGTTCTCCATCATTCCCGGTAATAAGGTCATGCAAATTTAACTACGTAAAATCGCCGCTGCTGTGTCCTGATCGGTAACCAGTGCGTTGATATAACCGCCTTTCATTGCAGCGGCAATTGCTTCGGCTTTATTTTCTCCCCCTGCCACGCCAACCCGGACGGGTATg                                           <  1:96433/232‑1 (MQ=255)
                                                                                                                                                                              gggTAAAGA‑GTCGAGCCATTAGATTATCCTCGGCTTATGCGTtttgtctn                                                                                                                                                                                                                            <  1:42225/50‑2 (MQ=255)
                                                                                                                                                                                    aga‑gTCGAGCCATTAGATTATCCTCGGCTTATGCGTTTTGTCTGTTCCTCTATACGTTCTCCATCATTCCCGGGAATAAGGTCATGCAAATTTAACTACGTAAAATCGCCGCTGCTGTGTCCTGATCGGTAACCAGGGCTTTGAGATAACCGCCTTTCATTGCAGCGGCAATTGCTTCGGCTTTATTTTCTCCCCCTGCCACGc                                                            <  1:65213/204‑1 (MQ=255)
                                                                                                                                                                                      a‑gTCGAGCCATTAGATTATCCTCGGCTTATGCGTTTTGTCTGTTCCTCTATACGTTCTCCATCATTCCCGGTAATAAGGTCATGCAAANNTAACTANNt                                                                                                                                                                   >  2:18503/1‑99 (MQ=255)
                                                                                                                                                                                               naTTAGATTATCCTCGGCTTATGCGTTTTGTCTGTTCCTCTATACGTTCTCCATCATTCCCGGTAATAAGGTCATGCAAATTTAACTACGTAAAATCGCCGCTGCTGTGTCCTGATCGGTAACCAGTGCGTTGATATAACCGCCTTTCATTGCAGCGGCAATTGCTTCGGCt                                                                                  >  1:264988/2‑172 (MQ=255)
                                                                                                                                                                                                aTTAGATTATCCTCGGCTTATGCGTTTTGTCTGTTCCTCTATACGTTCTCCATCATTCCCGGTAATAAGGTCATGCAAATTTAACTACGTAAAATCGCCGCTGCTGTGTCCTGATCGGTAACCAGTGCGTTGATATAACCGCCTTTCATTGCAGCGGCAATTGCTTCGGCTTTATTTTCTCCCCCTGCCACGCCAACCCGGACGGGTATGGTCTTCAGCGCGCTTAAAGGTAAGCCAATCAGTTCGttctg  <  1:18503/251‑4 (MQ=255)
                                                                                                                                                                                                             nngCTTATNNGTTTTGTCTGTTCCTCTATACGTTCTCCATCATTCCCGGTAATAAGGTCATGCAAATTTAACTACGTAAAATCGCCGCTGCTGTGTcc                                                                                                                                              <  2:26213/96‑1 (MQ=255)
                                                                                                                                                                                                                          |                                                                                                                                                                                                                                
GTTGCCAGTTTTTGTTGAGATCAAATTCCATAGAACCAGGAACGTCCGGTACTGCCAGATGCCGCCACTCCGCCTGTCCCACTGCTATTTGATTGCCTTCCAGGTCGAATATCACAGCCCGAATACTTCCGGTGCCTGCATCCAGCGCCATCAGGTAGTACTTTGATTCTGAAAGGGTAAAGA‑GTCGAGCCATTAGATTATCCTCGGCTTATGCGTTCTGTCTGTTCCTCTATACGTTCTCCATCATTCCCGGTAATAAGGTCATGCAAATTTAACTACGTAAAATCGCCGCTGCTGTGTCCTGATCGGTAACCAGTGCGTTGATATAACCGCCTTTCATTGCAGCGGCAATTGCTTCGGCTTTATTTTCTCCCCCTGCCACGCCAACCCGGACGGGTATGGTCTTCAGCGCGCTTAAAGGTAAGCCAATCAGTTCGTTATG  >  NC_000913/1600017‑1600458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑