Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,553,310	C→T	A177T (GCC→ACC)	adhP ←	ethanol dehydrogenase/alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,553,310	0	C	T	100.0%	30.4 / NA	14	A177T (GCC→ACC)	adhP	ethanol dehydrogenase/alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/8);  total (6/8)

CCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCTACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATCGCTAAATCTGCGCCCATTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCG‑CGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCG  >  NC_000913/1553063‑1553537                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                    ccGACAGCCACAACCCGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCTACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAAGCGCTAAATCTGCGCCCATTTCGGTTGCCAGTTTGAACTGCTCATCATTGACATCAATGGCGATCACTGTGGCGTTAAAGACATTCTTCG‑CGTATTGCAGGGTGAg                                                                                                                                                                                                                                  <  1:363187/251‑1 (MQ=255)  cGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCTACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATCGCTAAATCTGCGCCCATTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCG‑CGTATTGCAGGGTGAgg                                                                                                                                                                                                                                 <  1:256057/251‑1 (MQ=255)                                        aCTGCCGAGTTAAACGCAGCTTTAGCTACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCCCAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATCGCTAAATCTGCGCCCCTTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCG‑CGCATTGCAGGGTGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTg                                                                                                                                                                                           >  1:69155/1‑251 (MQ=255)                                                          gCTTTAGCTACCTCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATCGCTAAATCTGCGCCCATTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCG‑CGTATTGCAGGGTGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTg                                                                                                                                                                          >  1:327866/1‑250 (MQ=255)                                                          gCTTTAGCTACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATCGCTAAATCTGCGACCATTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCG‑CGTATTGCAGGGTGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTg                                                                                                                                                                          >  1:16435/1‑250 (MQ=255)                                                           cTTTAGCTACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATCGCTAAATCTGCGCCCATTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCG‑CGTATTGCAGGGTGAGGTTACCCAGACCGc                                                                                                                                                                                                                    <  1:289117/207‑1 (MQ=255)                                                                                     ncGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATCGCTAAATCTGCGCCCATTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCG‑CGTATTGCAGGGTGAGGTTACCCAGAcc                                                                                                                                                                                                                      >  1:107369/2‑179 (MQ=255)                                                                                     gCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATCGCTAAATCTGCGCCCATTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCG‑CGTATTGCAGGGTGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGAc                                                                                                                                              >  1:353555/1‑251 (MQ=255)                                                                                                                                               tAATCGCTAAATCTGCTCCCATTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCTTTAAAGACATTCTTCG‑CGTATTGCAGGGTGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTAc                                                                                     <  1:290421/250‑1 (MQ=255)                                                                                                                                                             gcgccctgttCGGTTGCCATTTGTAACTGCTCATCATTGACATTAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGGCG‑ATGCCAGGGTGAGGTTACCCAGACCGCCAAGGCCGTAGAGAGCAAACCACTGCCCTTGACGAATTTTTGACAGCTTAGCGTCTTTGTAGGTGGTGTGCCCCGCACAGGTAACGCTGCGGGCCGCCGCCTAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGAc                                                                      <  1:239519/243‑1 (MQ=255)                                                                                                                                                                                    tAACTGCTCATCATGGACATCAAGGGCGATCACTTTGGCGTTAAAGACATTCTTCG‑CTTATTGCAGGGTGAGGTTACCCAGACCGCCAAGACCTTAGATAGCAATCCACGGCCCTGGACGAATTTTTGACAGCTGAACGGCTTTGTAGGGGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAg                                                                                                            <  1:361170/190‑1 (MQ=255)                                                                                                                                                                                     aaCTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCG‑CGTATTGCAGGGTGAGGTTACCCAGACCGCCAAGACCTTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATTCACTCTTccgccatcc                                               <  1:366794/250‑1 (MQ=255)                                                                                                                                                                                                                          ctttAAAGACATTCTTCG‑CGTATTGCAGGGTGAGGTTACCCAGACCGCCCAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGgccgcc                                                                                                                  >  1:407214/3‑146 (MQ=255)                                                                                                                                                                                                                                                   gCAGGGTGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACGCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACGACGATGCACTCGTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCg  <  1:244056/234‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                    CCGACAGCCACAACACGACCGCCTGCACGGACAGCATCAACTGCCGAGTTAAACGCAGCTTTAGCTACCGCTGTTACCACCGCAGCGTGAGCGCCACCAGTTTTCTCCTGCACAATTTTGGCGGCGTCTTCGGTGTGTGAGTTAATCGCTAAATCTGCGCCCATTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCG‑CGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCG  >  NC_000913/1553063‑1553537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑