Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,479,799 C→T P221L (CCC→CTC)  ynbC → hydrolase/methyltransferase domain‑containing protein YnbC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,479,7990CT100.0% 60.3 / NA 22P221L (CCC→CTC) ynbChydrolase/methyltransferase domain‑containing protein YnbC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/10);  total (12/10)

TTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGAACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGG  >  NC_000913/1479562‑1480044
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     
ttAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTAGGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTTAACGTATTATTAGAGATGCTGCGTCTATTACGCTCCTCACGCAACTTCt                                                                                                                                                                                                                                           <  1:367340/250‑1 (MQ=255)
         aaTTGTATGTGCCGCTGGCACTTCCTGCTCTGGCGGTATGGTATCGTCTGCGTGGTCTGTGTTTTATTAATTCCTATTTGAAAGGACGCTATTTGACCCACGATCTGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCTCACGGGCGATTGCCGTGAATATCTTTCTCGATCTCTAGAAAGCGTCTTAACGGATTAGTAGAGATGCGGCGGCGATTACTCTCCTCAGGCAACTTCTGATATCAGGc                                                                                                                                                                                                                                 <  1:187125/251‑1 (MQ=255)
                                                          gCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGACGATGGCCTTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCTCACTCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCagag                                                                                                                                                                                  <  1:434517/249‑1 (MQ=255)
                                                                                 ccTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCGCCTCACACAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCt                                                                                                                                                          <  1:425448/250‑1 (MQ=255)
                                                                                               cGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCTCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCCCTGAAAGAGCTGCATCTGCTGCCAgg                                                                                                                                           >  1:104316/1‑251 (MQ=255)
                                                                                                         cccACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCTCACGCAACTACTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGAtt                                                                                                                                                                               >  1:169136/1‑205 (MQ=255)
                                                                                                          ccACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCTCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAgn                                                                                                                                                                        <  1:117856/211‑2 (MQ=255)
                                                                                                                   gCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGCTCTCTACAAACCGTATGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCTCCCGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGCTTACGTAGCCCTCTGAAAGAGCTGCATctgc                                                                                                                                                  >  1:316250/1‑224 (MQ=255)
                                                                                                                             gCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCTCACGCAACTTCTGATATCAGNCGANNNNNAt                                                                                                                                                                                                                        >  2:276353/1‑144 (MQ=255)
                                                                                                                                 gTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAACACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCTCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACAGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGAACAgg                                                                                                         >  1:226566/1‑251 (MQ=255)
                                                                                                                                          atGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCTCACGCAACTTCTGATATCAGGCGATGACTa                                                                                                                                                                                                                         <  1:22146/130‑1 (MQ=255)
                                                                                                                                                   tGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGAAGCGGCGGCGATTACGCTCCTCACGCAAATTATGATATCAGGCGATGACTATGCGGTGCCTCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAAAGCTGCCTCTGCTGCCAAGCTTTTATCCCGACCCGTTggga                                                                                                                     >  1:180181/1‑220 (MQ=255)
                                                                                                                                                                gATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGAGGCGGCGATTACGCTCCTCACGCAACTTCTGATATCAGGCGATGACTATGTGATGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGAACAGGGCGCTGGCATTTGAAAAAATGCAAAGCttt                                                                           >  1:285277/1‑250 (MQ=255)
                                                                                                                                                                                   cTCGATCTCTACAAAACGTCTGAACGTATTATTAGCGATGCCGCGGCGATTACGCTCCTCACGCAACTTCTGATATCAGGCGATGACTATATGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGAACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTa                                                        >  1:180816/1‑250 (MQ=255)
                                                                                                                                                                                     cGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCTCACGCAACTTCTGATATCAGGCGNTGANTATGNGGNGCATCGCCAACCGCANATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCANNTNNNGNCAGGCttt                                                                                                                                       <  2:311015/169‑1 (MQ=255)
                                                                                                                                                                                     cGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCTCACGCAACTTCTGATATCAGGCGCTGACTATGTGGTGCATCGCCAACCGCCGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCttt                                                                                                                                       >  1:311015/1‑169 (MQ=255)
                                                                                                                                                                                       aTCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCTCACg                                                                                                                                                                                                                                                   <  1:10017/59‑1 (MQ=255)
                                                                                                                                                                                       aTCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCTCACg                                                                                                                                                                                                                                                   >  2:10017/1‑59 (MQ=255)
                                                                                                                                                                                          nctNCAANANNTNNGAANGTATTATTAGAGATGCGGCGGNNATTACGCTCNTCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGNTTGATTTTTNNCANAGATTNCNNNGNNNCCNGAAAGAGCTGCATCTGCTGNCANNCTTTTANCACGACACGTTGGGTGAAGAGAACAg                                                                                                          <  2:104316/192‑1 (MQ=255)
                                                                                                                                                                                           ctACAAAACGTCTTAACGTAGTATTAGAGCTGAGGCGGCGCCTACGCTCCTCACGCAAATTATGATATCAGGCGATGACTATGTTGTGCATCGCCATCCGCAGATTGATTTTTATCAGAGATTCCGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGTGTGAAGAGAACAGTGCGCTTGCATTTAAAAAAATGCAAATCTTTATTAGTCGTTTATATGCTAACAAAt                                                  <  1:17020/248‑1 (MQ=255)
                                                                                                                                                                                                                             gcggcgATTACGCTCCTCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCACACGCCGATTGATTTTTTTCCGAGCTTACGTAGCCctct                                                                                                                                                                   >  1:71824/1‑101 (MQ=255)
                                                                                                                                                                                                                                       cGCTCCTCACGCAACTTCTGATCTCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGAACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATc                                >  1:124397/1‑222 (MQ=255)
                                                                                                                                                                                                                                         ctcctcACGCAACTTCTGATATCAGGCGATGACTATGTGATGCATCGCCAACCGCAGATTGATTTTTATAAGAGATTACGTAACCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGAACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGATAACAAATCGCAAAAATTTGATTATCAGAATGAAGACTGCACAGGACCATCAGCgg  >  1:191794/1‑250 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     
TTAAGGTTAAATTGTATGTGCCGCTGGCACGTCCTGCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTGATATCAGGCGATGACTATGTGGTGCATCGCCAACCGCAGATTGATTTTTATCAGAGATTACGTAGCCCTCTGAAAGAGCTGCATCTGCTGCCAGGCTTTTATCACGACACGTTGGGTGAAGAGAACAGGGCGCTGGCATTTGAAAAAATGCAAAGCTTTATTAGTCGTTTATATGCTAACAAATCGCAAAAATTTGATTATCAGCATGAAGACTGCACAGGACCATCAGCGG  >  NC_000913/1479562‑1480044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑