Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	246,747	G→T	S12S (TCG→TCT)	yafL →	NlpC/P60 family protein YafL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	246,747	0	G	T	100.0%	59.4 / NA	22	S12S (TCG→TCT)	yafL	NlpC/P60 family protein YafL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/10);  total (12/10)

GGGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATA  >  NC_000913/246504‑246957                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                   ggggTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAATTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTAc                                                                                                                                                                                                             <  1:208514/251‑1 (MQ=255)    gTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGt                                                                                                                                                                                                          >  1:33332/1‑251 (MQ=255)                        ttattCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTttc                                                                                                                                                                                       <  1:297777/250‑1 (MQ=255)                                         tAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGGTTTTAACTAATTAACTGAAATATATATGTTAATGTTCTCATAATGGTTTATTAAGGTTGTTCAGAACAGATCCGGGATACACTGAAACATTAAAGCATGACCAGACATTATTGAGTATGTCCTTGACGTCGATTCCATGCTTTGTATTGTATGGATTACTGTTGGTTTGTTTGCCGTTTTCTTCATTTGCCAGAGccac                                                                                                                                                                     >  1:405980/1‑251 (MQ=255)                                            cgcAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATTACCAGCCATTATTGAGTATGTCCGTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTttcttc                                                                                                                                                                                    <  1:193682/231‑1 (MQ=255)                                                                                    atcgaactggatgactttgccgaCTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTTTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCTATTCCATCCTTTGTATTGTCTGGATTACTTTTGATTGGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATg                                                                                                                          <  1:401402/229‑1 (MQ=255)                                                                                                tACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTTTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTgc                                                                                                               <  1:380353/250‑1 (MQ=255)                                                                                                                                 ataataATGGTTTATTAAGTTGGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGGCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGGTGGCCGTTTTCTGCATTTGCCAGCACCACCACATCACGTTTCTCTTTTTGCTACGCCGCTCGCCGGCGGATGCAAAGCCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaaa                                                                             <  1:249439/251‑1 (MQ=255)                                                                                                                                                       gttaaGAACNGATCCGGGATACCCGACAACATAAAAGCATTNCNAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTNTGGATTACTGNTGANNTGTTNGNCNTGTTCTTCATTTGCCAGCGCCACCACATCNCATATCTCTTTCAGCTACGCCTCNNGCNNNCGNNTGCaa                                                                                                                       <  2:337341/183‑1 (MQ=255)                                                                                                                                                       gTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTCTGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCaa                                                                                                                       >  1:337341/1‑187 (MQ=255)                                                                                                                                                       gTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGAATGTACTTGCCGACGATTACATCCTTTGTATTGTATGGCTTACTGTTGATTTGTTTACCGTTTTCTTCATTTGCCCGCGcc                                                                                                                                                                       >  1:418194/1‑139 (MQ=255)                                                                                                                                                          naGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCaa                                                                                         >  1:282076/2‑214 (MQ=255)                                                                                                                                                                            cacTGCAACATTACAGCCTGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGc                                                                                                                                                                                              >  1:370147/1‑95 (MQ=255)                                                                                                                                                                              cTGCAACATTAAAGCATGACCCGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTAcg                                                                                                                                           >  1:343466/1‑144 (MQ=255)                                                                                                                                                                                  aaCATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGCTTACTGTTGCTTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCCACGGATGCAAAAc                                                                                                                    >  1:398823/1‑163 (MQ=255)                                                                                                                                                                                       tAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAgg                                                                      <  1:213725/204‑1 (MQ=255)                                                                                                                                                                                                      cATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTa                                                                                             >  1:9186/1‑166 (MQ=255)                                                                                                                                                                                                             gAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCTCCGCCACCCCATCACATCTCTCTTTAAGCTACGCCGCCAGCCAGCGGATGCAAAACCGTGCGCGTTTAATAAAACAGTACCACACTCATCTGAAAACGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAACGGGCGCTACGCCAGCACAACCGGGAGCAGATa  >  1:431214/1‑250 (MQ=255)                                                                                                                                                                                                               gTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGCTTTGTTTGCCGTTTTATTCATTTGCCAGCGACACCACTTCAAATAACTCTTTCAGCTACGCCGCCCGCCAGCGCATGCAAAAc                                                                                                                    >  1:212692/1‑134 (MQ=255)                                                                                                                                                                                                                          ccGTCGATTCCATCCTTTTAATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTTATTTGCCAGCGCCCCCACATCACATATCTCtttt                                                                                                                                                  >  1:158595/1‑92 (MQ=255)                                                                                                                                                                                                                                    nnnccTNTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCCCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCaa                                                                                         <  2:282076/137‑1 (MQ=255)                                                                                                                                                                                                                                       ccTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAgg                                                                      <  1:356014/156‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                   GGGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATA  >  NC_000913/246504‑246957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑