Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,998	A→G	S273G (AGC→GGC)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,998	0	A	G	100.0%	37.4 / NA	13	S273G (AGC→GGC)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/4);  total (9/4)

CCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGC  >  NC_000913/1301828‑1302231                                                                                                                                                                           |                                                                                                                                                                                                                                          ncTATACCTTAAAAGATTGGGTCGTAACCGAACGAATAGCTCTTGAACGCAGCCCGCCCTCCTGGCACACCGCGAAACCCGTTATTAACCAGGTAACCTATTTGACTATTGCTTCTGAACTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATACCg                                                                                                                                                                                                                                   >  1:48625/2‑179 (MQ=255)                                                    ncccGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATgtgcgtgtgcgt                                                                                                        >  1:273204/2‑251 (MQ=255)                                                      ccGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACAAGAAACCGCCATTCAACGATGTGCGTGTGCGTAc                                                                                                      >  1:298524/1‑251 (MQ=255)                                                              cTGGAACAACNCNAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTNCCGATGTCAANCGCNNCCGTNGNGNTGAAAGCGACAGGACGAATAACGGCATGCNGATCGAATTGTTCCAGAAGCTGANNAANNNGANNCCGGa                                                                                                                                                                                             <  2:332085/156‑1 (MQ=255)                                                              cTGGAACAACGAGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTCCCGATGTCAACACCTACCGTAGTGGTGAAATAGACATGACGAATAACGGCATGCCGATCGAATTGTTACAGAAGCTGAAAAAAGAGATCCCGGa                                                                                                                                                                                             >  1:332085/1‑156 (MQ=255)                                                                                          cAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGAc                                                                                >  1:256186/1‑237 (MQ=255)                                                                                                         ccTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTAc                                                                                                                                                     >  1:12960/1‑153 (MQ=255)                                                                                                                cTTCTGAAGTTACCGATGTCAACCGCTACCGTCGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGCAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGAACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAATGTGAAAGCGCAGGGc                                            >  1:161421/1‑251 (MQ=255)                                                                                                                     naaGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGaaa                                                                                            >  1:108630/2‑198 (MQ=255)                                                                                                                                            ccGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGATAAAATAGATcnc                                                                                                                                                                                                <  1:348059/75‑1 (MQ=255)                                                                                                                                                   ggtgAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATAc         >  1:309847/1‑251 (MQ=255)                                                                                                                                                           cGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTGGATCCATCCCTTTGCACTTACTATAACGAAATTAACAACCAAAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAATTAGGTATGGACCGCAATATCATTGTTAATAAAGTGAAAGTGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGgc  <  1:419427/250‑1 (MQ=255)                                                                                                                                                               aTGACGAATAACGGCATGCCGAACGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCGTTGCACTTACTATTACGAAAGTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACcgc                                                                             <  1:434659/171‑1 (MQ=255)                                                                                                                                                                           |                                                                                                                                                                                                                                          CCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGC  >  NC_000913/1301828‑1302231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑