Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,181,285	C→T	R147R (AGG→AGA)	ymfA ←	putative inner membrane protein YmfA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,181,285	0	C	T	100.0%	29.5 / NA	13	R147R (AGG→AGA)	ymfA	putative inner membrane protein YmfA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/7);  total (6/7)

GACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAGCTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTCAGAGGCAGGAGTATCAGTAAACTGAGCAGTATAATGAATTTTTTCATGGGCGGGACTTCCGTTTCCTGCGTGATTTTTTAGAGGAGGGCATACTTATTAATAAACCGACGGCGATAAAAATAACGCCCATAATGCCGATAAACCCCCATTTAAAAAAATTAACCCACGGGTTATTTATCTCAGCCTTTTCAGGATGCCCGGGAGAATAATAAACATTAACTTTATCGCCTTCGCTGTCTTCATAAAAATCGCTGCCGATACTCGAATTGAAAATTAGCGTGTAATCTGGCGTCGGTC  >  NC_000913/1181051‑1181517                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                         gaCTTGCTTCATTATTAATTTCGGGTAAAACCACAATAAACGTTGCAATTCCCGTTTGTGGGCGAAAAATCTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGGCCTTAAAAAGTGTCTGCATTATTGGAATTAATGGCTTGCTGTCTGCTGTCAGAGGCAGGAGTATCAGTAAACTGAGCAGTATAATGAAGTTTTACATGGGCGGGACTTCCGTTTTCTGCGTGATTTTTTan                                                                                                                                                                                                                          <  1:79669/251‑2 (MQ=255)                                                        ttgttgGCGAAAAAGCTCGGCTTTATGCGCGATCGCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATGGGAATTAATGGCTTGCTGGCGGCTGTCAGAGGCAGGAGTATCAGTAAACTGAGCAGTATAATGAATTTTTTCATGGGCGGGACTTCCGTTTTCTGCGTGATTTTTTAGAGGAGGGCATACTTATTAATAAACCGACGGCGATAAAAATAACGCCCATAATGc                                                                                                                                                                    <  1:74998/250‑1 (MQ=255)                                                         tgttgGCGAAAAAGCTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTCAGAGGCAGGAGTATCAGTAAACTGAGCAGTATAATGAATTTTTTCATGGGCGTGACTTCCGTTTTCTGCGTGATTTTTTAGAGGAGTGCATACTTATTAATAAACCGACGGCGATAAAAATAACGCCCATAATGcc                                                                                                                                                                   <  1:119023/250‑1 (MQ=255)                                                              ncGAAAAAGCTCGGCTTTATGCGCGATCTCTTCAGTATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTCAGAGGCAGGAGTATCAGTAAACTGAGCAGTATAATGAATTTTTTCATGGGCGGGACTTCCGTTTTCTGCGTGATTTTTTAGAGGAGGGCATACTTATTAATAAACCGACGGCGATAAAAATAACGCCCATAATGCCGATaa                                                                                                                                                              >  1:46594/2‑250 (MQ=255)                                                               cGAAAAAGCTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTCAGAGGCAGGAGTATCAGTAAACTGAGCAGTATAATGAATTTTTTCATGGGCGGGACTTCCGTTTTCTGCGTGATTTTTTAGAGGAGGGCATACTTATTAATAAACCGACGGCGATAAAAATAACGCCCATAATGCCGATAAAc                                                                                                                                                            >  1:139790/1‑251 (MQ=255)                                                                                      gATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAATAGTGTCTTCATTATTGGAAGTAATGGCTTGCTGGCTGCTGTCAGAGGCTGGAGTATCAGTTAACTGAGCAGTATAATTAATTTTTTCGTGTGCGGGACTTCCGTTTTCTGCGTGATTTTTTAGAGGAGGGCATACTTATTAATAAAcc                                                                                                                                                                                                 <  1:242163/191‑1 (MQ=255)                                                                                                 cATCTGGTACAGTACAGCTAACGTACGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTCAGAGGCAGGAGTATAAGTAAACTGAGCAGTATAATGAATTTTTTCATGGGCGGGCATTCCGTTTTCTGCGTGATTTTTTAGAGGAGGGCATACTTATTAATAAACCGACGGAGATAAAAATAACGCCCATAATGACGATAAACCCCCATTTAAAAAAATTAACCCAAGGGttattt                                                                                                                           >  1:147185/1‑250 (MQ=255)                                                                                                                                    ttCTTCATTATTGGAATTAATGGCTTTCTGGCTGCTGTCAGAGGCAGGAGTATCAGTAAACTGAGCATGATAATGAATTTGTTCATGGGCGGGACTTCCGTTTTCTGCGTGATTTTTTATAGGAGGTCATACTTATTAATAAACCGACGGCGATAAAAATAACGCCCATAATGCCGATAAAcc                                                                                                                                                           <  1:59277/182‑1 (MQ=255)                                                                                                                                                         ggCTTGCTGGCTGCTGTCAGAGGCAGGAGTATCAGTAAACTGAGCAGTATAATGAATTTTTTCATGGGCGGGCCTTCCGTTTTCTGCGTGATTTTTTAGAGGAGGGCATACTTATTAATAAACCGACGGCGATAAAAATAACGCCCATAATGCCGATCAACCCCCATTTAAAAAAATTAACCCACGGGTTATTTATCTCAGCCTTTTCAGGATGCCCGGGAGAATAATAAACATTAACTTTATCGCCTTCg                                                                  >  1:260322/1‑251 (MQ=255)                                                                                                                                                         ggCTTGCTGGCTGCTGTCAGAGGCAGGAGTATCAGTAAACTGAGCAGTATAATGAATTTTTTCATGGGCGGGACTTCCGTTTTCTGCGTGATTTTTTAGAGGAGGGCATACTTATTAATAAACCGACGGCGATAAAAATAACGCCCATAATGCCGATAAACCCCCATTTAAAAAAATTAACCCACGGGTTATTTAtctc                                                                                                                      <  1:213705/199‑1 (MQ=255)                                                                                                                                                          gCTTGCTGGCTGCTGTCAGAGGCAGGAGTATCAGTAAACTGAGCAGTATACTGAATTTTTTCATGGGCGGGACTTCCGTTTTCTGCGTGATTTTTTAGAGGAGGGCATACTTATTAATAAACCGACGGCGATAAAAATAACGCCCATAATGCCGATAAACCCCCATTTAAAAAAATTAACCCACGGGTTATTTATCTCAGCCTTTTCAGGATGCCCGGGAGAATAATAAACATTAACTTTATCGCCTTCGc                                                                 >  1:426471/1‑251 (MQ=255)                                                                                                                                                                        gTCAGAGGCAGGAGTATCAGTAAACTGAGCAGTATAATGAATTTTTTCATGGGCGGGACTTCCGTTTTCTGCGTGATTTTTTAGAGGAGGGCATACTTATTAATAAACCGACGGCGATAAAAATAACGCCCATAATGCCGATAAACCCCCATTTAAAAAAATTAACCCACGGGTTATTTATCTCAGCCTTTTCAGGATGCCCGGGAGAATAATAAACATTAACTTTATCGCCTTCGCTGTCTTCATaaaa                                                    >  1:406378/1‑250 (MQ=255)                                                                                                                                                                                                                            gCGGGACTTCCGTTTTCTGCGTGATTTTTTAGAGGAGGGCATACTTATTAATAAACCGACGGCGATAAAAATAACGCCCATAATGCCGATAAACCCCCATTTAAAAAAATTAACCCACGTGTTATTTATCTCAGCCTGTTCAGGATGCCCGGGAGAATAATAAACATTAACTTTATCGCCTTCGCTGTCTTCATAAAAATCGCTGCCGATACTCGAATTGAAAATTAGCGTGTAATCTGGCgtcggtc  <  1:418760/248‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                         GACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAGCTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTCAGAGGCAGGAGTATCAGTAAACTGAGCAGTATAATGAATTTTTTCATGGGCGGGACTTCCGTTTCCTGCGTGATTTTTTAGAGGAGGGCATACTTATTAATAAACCGACGGCGATAAAAATAACGCCCATAATGCCGATAAACCCCCATTTAAAAAAATTAACCCACGGGTTATTTATCTCAGCCTTTTCAGGATGCCCGGGAGAATAATAAACATTAACTTTATCGCCTTCGCTGTCTTCATAAAAATCGCTGCCGATACTCGAATTGAAAATTAGCGTGTAATCTGGCGTCGGTC  >  NC_000913/1181051‑1181517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑