Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,169,836	A→G	L180P (CTG→CCG)	ldtC ←	L,D‑transpeptidase LdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,169,836	0	A	G	100.0%	27.9 / NA	10	L180P (CTG→CCG)	ldtC	L,D‑transpeptidase LdtC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/6);  total (4/6)

GGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATC  >  NC_000913/1169590‑1170060                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                 ggCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGCCAGAGACTTTTATCGCAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGAGTTTTGATATCGTCCTCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATAGGGTTAtccggtcc                                                                                                                                                                                                                              >  1:242218/1‑251 (MQ=255)                                                     ggggggtgtTGATGATATTCTGTTTGGTGCCGGGGGTGCCCGGGCTAAAGAGTTTTTTGATAGCGTCAGCCCGCAGCCGAATACAGCCAGACCTGACCCGCATGCCAAGGCCGAAATCGGCGTTGGTACCATGAAGCAAAGAAACGCCGCCATAGGCGGCCAGAGGAAGCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGc                                                                                                                                                                                                           <  1:240319/213‑1 (MQ=255)                                                        gAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTTGCGAAAGAGTGTTTTGATATCTTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTGCCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCGCATTGGGTTATGCGGTCCAGCCGTCCCTACCGCAGGCAACTCAATGCCCTGTGCTTTATAACGGGCGCGGATg                                                                                                                                                                       <  1:405967/251‑1 (MQ=255)                                                                                       ggTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCAc                                                                                                                                        <  1:65013/251‑1 (MQ=255)                                                                                                                                                     cccGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGCCGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCCATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAAc                                                                                   >  1:440114/1‑242 (MQ=255)                                                                                                                                                                                                aTAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGCTATCCGGTCCACCCGGCCCTACCGCAGGCAACTCAATTCTCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTACAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACAAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGgtagt                               >  1:257184/1‑251 (MQ=255)                                                                                                                                                                                                         gccATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGAc                      >  1:425929/1‑251 (MQ=255)                                                                                                                                                                                                              aGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAgn                 <  1:130882/251‑2 (MQ=255)                                                                                                                                                                                                                       gACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTgn                                                                                                                                                            <  1:110754/103‑2 (MQ=255)                                                                                                                                                                                                                              ngCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCCCCTa                                                                                     >  1:79348/2‑167 (MQ=255)                                                                                                                                                                                                                              cGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCTCCTAACTGACCAATTCCTATTGGATACACGGTTACCGAATTTTTACCCGGCTGGTATTAATAGATACGCAGCTCCGCAATGTTGATc  <  1:372022/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                 GGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATC  >  NC_000913/1169590‑1170060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑