Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,155,389	C→T	A89V (GCT→GTT)	tmk →	Tmk

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,155,389	0	C	T	100.0%	22.7 / NA	9	A89V (GCT→GTT)	tmk	Tmk
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/4);  total (5/4)

CCTGGCGGTACGCAACTTGCCGAAAAGTTAAGAAGCCTGGTGCTGGATATCAAATCGGTAGGCGATGAAGTCATTACCGATAAAGCCGAAGTTCTGATGTTTTATGCCGCGCGCGTTCAACTGGTAGAAACGGTCATCAAACCAGCTCTGGCTAACGGCACCTGGGTGATTGGCGATCGCCACGATCTCTCCACTCAGGCGTATCAGGGCGGCGGACGTGGTATTGACCAACATATGCTGGCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGC  >  NC_000913/1155244‑1155585                                                                                                                                                  |                                                                                                                                                                                                     ccTGGCGGTACGCAACTTGCCGGAAAGTTAAGAAGCCTGGTGCTGGATATCAAATCGGTAGGCGATGAAGTCATTACCGATAAAGCCGAAGTTCTGATGTTTTATGCCGCGCGCGTTCAACTGGTAGAAACGGTCATCAAACCAGTTCTGGCTAACGGCACCTGGGTGATTGGCGATCGCCACGATCTCTCCACTCAGGCGTATCa                                                                                                                                          >  1:153490/1‑206 (MQ=255)     gCGGTACGCAACTTGCCGAAAAGTTAAGAAGCCTGGTGCTGGATATCAAATCGGTAGGCGATGAAGTCATTACCGATACAGCCGAAGTTCTGATGTTTTATGCCGCGCGCGTTCAACTGGTAGAAACGGTCATCAAACCAGTTCTGGc                                                                                                                                                                                                >  1:75300/1‑148 (MQ=255)                                                            aGGCGATTAAGTCATTACCGATAAAGCCGAAGTTCTGATGTTTTATGCCGCGCGCGTTCAACTGGTAGAAACGGTCATCAACCCAGTTCTGGCTAACGGCACCTGGGTGATTGGCGATCGCCACGATCTCTCCACTCAGGCGTATCAGGGCGGCGGACGTGGTATTGACCAACATATGCTGGCAACACTGCGTTATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCgn                                  <  1:56324/251‑2 (MQ=255)                                                                                             tCTGATGTTTTATGCCGCGCGCGTTCAACTGGTAGAAACGGTCATCAAACCAGTTCTGGCTAACGGCTCCTGGGTGATTGGCGATCGCCACGATCTCTCCACTCAGGCGTATCAGGGCGGCGGACGTGGTATTGACCAACATATGCTGGCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGTCTTAACTCTCTATCTCGATGTTTCCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCgcggc  <  1:194445/250‑1 (MQ=255)                                                                                                         tGCCGCGCGCGTTCAACTGGTAGAAACGGTCATCAAACCAGTTCTGGCTAACGGCACCTGGGTGATTGGCGATCGCCACGATCTCTCCACTCAGGCGTATCAGGGCGGCGGACGTGGTATTGACCAACATATGCTGGCAACACTGCGTGATGCTGTTCTCggn                                                                             <  1:130579/163‑2 (MQ=255)                                                                                                                      cAACTGGTAGAAACGGTCATCAAACCNGTTCTGNNTANCGGCACCTGGGTGATTGGCNATNNNCNNNANCTCTCNACNNAGGCGTATCNGGGCGGCGGACGTGGTATTGACCAACATATGCTGGCAACACTGCGTGATGCTGNTCTCGGGGANNTTCGcc                                                                   >  2:98689/1‑160 (MQ=255)                                                                                                                      cAACTGGTAGAAACGGTCATCAAACCAGTTCTGGCTAACGGCACCTGGGTGATTGGCGATCGCCACGATCTCTCCACTCAGGCGTATCAGGGCGGCGGACGTGGTATTGACCAACATATGCTGGCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGcc                                                                   <  1:98689/160‑1 (MQ=255)                                                                                                                                    ggTCATCAAACCAGTTCTGGCTAACGGCACCTGGGTGATTGGCGATCGCCACGCTCTCTCCACTCAGGCGTATAAGGGCGGCGGACGTGGTAt                                                                                                                        >  1:210778/1‑93 (MQ=255)                                                                                                                                          naaaCCAGTTCTGGCTAACGGCACATGGATGATTGGCGATCGCCACGATCTCTCCACTCAGGCGTATCAGGGCGGCGGACGTGGTATTGAc                                                                                                                    >  1:359157/2‑91 (MQ=255)                                                                                                                                                  |                                                                                                                                                                                                     CCTGGCGGTACGCAACTTGCCGAAAAGTTAAGAAGCCTGGTGCTGGATATCAAATCGGTAGGCGATGAAGTCATTACCGATAAAGCCGAAGTTCTGATGTTTTATGCCGCGCGCGTTCAACTGGTAGAAACGGTCATCAAACCAGCTCTGGCTAACGGCACCTGGGTGATTGGCGATCGCCACGATCTCTCCACTCAGGCGTATCAGGGCGGCGGACGTGGTATTGACCAACATATGCTGGCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGC  >  NC_000913/1155244‑1155585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑