Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	891,983	T→A	L6* (TTG→TAG)	rimK →	ribosomal protein S6 modification protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	891,983	0	T	A	100.0%	30.4 / NA	12	L6* (TTG→TAG)	rimK	ribosomal protein S6 modification protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (5/7);  total (5/7)

AGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGAT  >  NC_000913/891764‑892211                                                                                                                                                                                                                            |                                                                                                                                                                                                                                     cgcaccTGGCGGAATATTCCCCCCCCCTGGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATACCCCCCGAACAATAATAAAAGAAAGCCCCCCATTTATTCTGGATTATTTCCACAAACAGGGGTGGGCGACGCGCAAAAACCGCCACGTCGATGTAGGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATAGTCCCGGGATGGAACGCTCTATTCGTGTAAg                                                                                                                                                                                                       <  1:165888/246‑1 (MQ=255)                                  gcaATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTCTTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATAGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCAc                                                                                                                                                                      >  1:203909/1‑251 (MQ=255)                                                               cATACCCGCAGAACAATCATAAAAGAAAGCCGCCAATTTATTTGGGATTATTTGCAAAAACGGGGTTGGGCGCCGCGCAAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATAGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTg                                                                                                                                         <  1:158647/251‑1 (MQ=255)                                                                                                                       caAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGCGAGGTGCAGGGTGAAAATTGCCATATAGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGacgc                                                                                                                                                                                         >  1:65879/1‑144 (MQ=255)                                                                                                                          ccAGGGTGGGGCGCCGCGCAAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATAGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCTGCTTCTTCTATTCGCTACAAAGGCCGCAAGTTAcccc                                                                              <  1:381684/250‑1 (MQ=255)                                                                                                                                          cgcTAACACCGCCACGTCGATGTATGATACGAGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATAGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACa                                                                                                >  1:357079/1‑217 (MQ=255)                                                                                                                                           gcTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATAGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTgg                                                                                                                                                                  >  1:409437/1‑150 (MQ=255)                                                                                                                                           acaaaaaCCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTTAAAATTGCCATATAGTCCCGGGATGGAACGCTCGATTCGGGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTTCATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATc                                                             <  1:245307/248‑1 (MQ=255)                                                                                                                                                                                ttGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGACATATAGTCCACGGATCGAACGATCTATTCGTGTAAGCGGCTGCGTAACGCCGCTCTACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTCTGGGAcgg                        >  1:386552/1‑251 (MQ=255)                                                                                                                                                                                            gACAGAGAGGTGCAGGGTAAAAATGGCCATATAGTCCCGGGAGGGAACGCTTTATTCGTGTAAGCGGCTGCTTGAAGCCGTTATACAGCGCGTTCACCTGTTTGAAATGCTTAATCCGCTTTCTGGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGTCCGCAAGTTCCCCCTTTTTGACGCAGTGAGCCCGCTTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGcc            <  1:310602/251‑1 (MQ=255)                                                                                                                                                                                                      tGCAGGGTGAAAATTGCCATATAGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGc                                                                                                                                                                                                   <  1:224659/58‑1 (MQ=255)                                                                                                                                                                                                       gCAGGGTGAAAATTGCCATATAGCCCCGGGGTGGAACGCTCTTTTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCCCCTGGTGTAAATTCTTGATCCGCTTTCTTGTTCCATGAACAGAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCGTTTTGACTCAGTGATCCCGCGTATTGGCACCGCCATTGCCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGAt  <  1:183627/250‑1 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                     AGCAACTGGCGGAATATTACCTCACCCGTGGCAGCAATAATCGCCGGGATACCTGGAGCGATCATATCCGCCGAACAATCATTAAAGAAAGCCGCCCATTTATTCTGGATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGAT  >  NC_000913/891764‑892211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑