Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	700,038	G→A	H97Y (CAT→TAT)	umpH ←	UMP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	700,038	0	G	A	100.0%	58.4 / NA	20	H97Y (CAT→TAT)	umpH	UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/10);  total (10/10)

GCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGG  >  NC_000913/699793‑700194                                                                                                                                                                                                                                                      |                                                                                                                                                             gctgggctTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACCAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGACCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGCTAATAGTGAAACCGGCTTTGTACAGTTCATAAATc                                                                                                                                                          >  1:224737/1‑250 (MQ=255)      gctTACCAACATAGAACGGTTTGCGACCGGAGCTTTTCTCAATCCCTGCACCCAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATGGGTAGCGATAAAACGTGCACCGTTAGCGACGCAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTgcg                                                                                                                                                    >  1:142689/1‑251 (MQ=255)                                 cGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACTACATACGCTTTCTTg                                                                                                                         <  1:126129/251‑1 (MQ=255)                                    agaTTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATTCGCTTTCTTGccn                                                                                                                      <  1:358285/251‑2 (MQ=255)                                                   cTGCACACAACGCGCCACAAGAGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGAGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAgg                                                                                                       >  1:299746/1‑251 (MQ=255)                                                             agcgcCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTACGGATTGGTGGCGATACAACGTGCACCGTTAGAGACGAAATAGGCTGCTTTATGCATCATGTCCAAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGATCACATCGGAAATAGTGAAACCGGATTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGc                                                                                               >  1:336402/2‑249 (MQ=255)                                                                         gggATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGc                                                                                   >  1:234876/1‑249 (MQ=255)                                                                                     gcgcCCGTGGGTGTCCGGATTGGTGGCGCTAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAac                                                                      >  1:377071/1‑250 (MQ=255)                                                                                                                   aaaaCGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATATTGAATCCGGCTTGGTACAGTTCAGAAATCAGTGCGCCGTCGCCCACCACATACGCTGTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTc                                                                                           <  1:74597/199‑1 (MQ=255)                                                                                                                            aCNNNNNGCGNCGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCccac                                                                                                                                         <  2:271399/144‑1 (MQ=255)                                                                                                                                    cgacgaAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGGACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGcc                         >  1:378123/1‑248 (MQ=255)                                                                                                                                                         gCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGACACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAgg  >  1:156923/1‑250 (MQ=255)                                                                                                                                                               tGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCGGGCGACGCAGGAAATCGGCAGTCGCCAtn                                                                                     <  1:111111/161‑2 (MQ=255)                                                                                                                                                                     aGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAAGCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGc                <  1:384380/224‑1 (MQ=255)                                                                                                                                                                              aaCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGGGCGCCGTCGCCCACCACATACGCTTTCTTTCCTTCCTGGCGACGCAGGAAATCCGCATTCGCCATCGCAGAGTTATAGAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTGCGCCAGATCt                   <  1:63697/212‑1 (MQ=255)                                                                                                                                                                                  cgTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCGTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACTCAGTAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGac                                                  <  1:299205/177‑1 (MQ=255)                                                                                                                                                                                        nccAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAAt                                                                                                   >  1:108264/2‑122 (MQ=255)                                                                                                                                                                                        nccAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGc                                 >  1:84439/2‑188 (MQ=255)                                                                                                                                                                                                  aCGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCGGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACAGCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGccc              <  1:75373/197‑1 (MQ=255)                                                                                                                                                                                                                                   ccGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCgg                                          <  1:376423/136‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                             GCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGG  >  NC_000913/699793‑700194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑