Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	690,948	G→A	V279V (GTC→GTT)	ybeX ←	CorC‑HlyC family protein YbeX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	690,948	0	G	A	100.0%	35.8 / NA	14	V279V (GTC→GTT)	ybeX	CorC‑HlyC family protein YbeX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/9);  total (5/9)

AGCGGCAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTGACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTG  >  NC_000913/690708‑691101                                                                                                                                                                                                                                                 |                                                                                                                                                          aGCGGCAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCt                                                                                                                                                 <  1:28331/251‑1 (MQ=255)  gCGGCAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCGAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTg                                                                                                                                                >  1:31827/1‑251 (MQ=255)                  gACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAGTAACGCCAGCAGCAGGCGAATGCGCTGGCGTGCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTGCGGTTTCATTCATCCAGCTTCTTCTGGGGTGAGTCATTCGGGATTTTAACATGAACCTGAATAATACGCCGACTg                                                                                                                                <  1:438716/251‑1 (MQ=255)                      gcggTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTGCAATTAATGAGGCAAAAGCCATGTAGTTAGCTATCCAGTTTCGGTTTTATTCATCCAGCTTCAGCTGTGGTGAGTCAGGCGTGATTTTAACATGAACCTGAATAATACGCCGACTGTCgg                                                                                                                            <  1:419033/251‑1 (MQ=255)                                                                           aGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGcc                                                                       >  1:237569/1‑251 (MQ=255)                                                                                                ccAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCACTTAATGAGGCAAAAGACATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGATGGGGTGAGTCCTCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGc                                                  >  1:297173/1‑251 (MQ=255)                                                                                                                                   agcagGCGAATGCGCTGGCGTTCAATTAAGGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTTGGGTGAGTCATCCGGGATTTGAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGTCACGTTGAACTGGTAACCGGCGATGACTATAGTCTGGCCACGCGGCGGAAGAAGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATc               <  1:220684/251‑1 (MQ=255)                                                                                                                                                                    gCAAAAGCNANNNANNTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTNAGTCATCCGGGATTTNAANATGANCCTNAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAg                                                                  >  2:309049/1‑167 (MQ=255)                                                                                                                                                                    gCAAAAGCCATGTAGTTATCGATCCAGTTTTGGTTTTATTCATCCAGCTTCGTCTGGGGTGAGTCATCCGGGATTTTAACATTATCCTTAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAg                                                                  <  1:309049/167‑1 (MQ=255)                                                                                                                                                                                                              tCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGGCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTg  <  1:319942/189‑1 (MQ=255)                                                                                                                                                                                                                                gAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCAGACTGTCGGCCATCGCCAATTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATg                                                               >  1:333520/1‑110 (MQ=255)                                                                                                                                                                                                                                gAGTCATCCGGGATTNTAACATGAACNTGANNAATANGNCNACTGTCGGCCATCGCCACTTTGAACTGGTNACCGTCGATGTCGATAGTTTCGCNNCGNNNCGNNAGATg                                                               <  2:333520/110‑1 (MQ=255)                                                                                                                                                                                                                                    cATCCGTGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGcc                                                                                                                          <  1:295371/47‑1 (MQ=255)                                                                                                                                                                                                                                    cATCCGGGNNTTNNANANGAACCNGAATAATACGCCGACTGTCGGcc                                                                                                                          >  2:295371/1‑47 (MQ=255)                                                                                                                                                                                                                                            aTTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTGTGAACTGGGAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAgtg                             <  1:395770/132‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                          AGCGGCAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTGACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTG  >  NC_000913/690708‑691101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑