Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	518,704	C→T	G149E (GGA→GAA)	ybbO ←	NADP(+)‑dependent aldehyde reductase YbbO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	518,704	0	C	T	100.0%	15.2 / NA	8	G149E (GGA→GAA)	ybbO	NADP(+)‑dependent aldehyde reductase YbbO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/5);  total (3/5)

CCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCATACATGCCGAATCCGGCATTGTTAAAGATCCCATACAGACAATTATCGGTCAGGGCGATCACC  >  NC_000913/518488‑518913                                                                                                                                                                                                                         |                                                                                                                                                                                                                  cccAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTGCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATTCCATCACCGATGATGTCATCACAATACGccn                                                                                                                                                                                    <  1:42054/248‑2 (MQ=255)                                                 cTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGAATATTTACTGGCCGCGTAAGCGACACGACCCGGCGTGGAGATTAATTCCATCACCGATGATGTCATCACAATACGCCCTTCCCCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTgc                                                                                                                                 >  1:447516/1‑251 (MQ=255)                                                                                             aCCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATTCCATCACCGATGATGTCATCACAATACGCCCTTCCCCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGATGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGc                                                                                     <  1:138222/251‑1 (MQ=255)                                                                                                           ggCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGAGGAGATTAATTCCATCACCGATGATGTCCTCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAActg                                                                                                         >  1:234592/1‑217 (MQ=255)                                                                                                                                                    gCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATTCCAGCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCTCACGGCTGATGGTGGAAAGGGGGCCATACATGCCGAATCCGGCATTGTTAAAGATCCCATa                              <  1:397330/251‑1 (MQ=255)                                                                                                                                                                                 aCTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATTCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCATACATGCCGAATCCGGCATTGTTAAAGATCCCATACAGACAATTATCGGTCAGGGCGATCAcc  <  1:33390/250‑1 (MQ=255)                                                                                                                                                                                    ggCCGNGTACGNGNNACGACCCGGCGTGGAGATTNATTCCATCACCGATGNTGTANTCACANTa                                                                                                                                                                                         >  2:186348/1‑64 (MQ=255)                                                                                                                                                                                       cgcgTAAGCGCCACGACCCGGCGTGGAGATTAATTCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTgcgc                                                                                                                               <  1:251798/119‑1 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                  CCCAACGTAAAGCGGGCGGCGATGCCGGGATTTTCGACTGGTTTATCACTTTGCGTCTGGTTGACGTTGTCGGTGAAGCGAGTACGAATGGGACCGGGTTCGATCAGGCTGACTTTAATTCCGCTGTGGCGCAGCTCCATGCGCAGTGCATCTGACCACGCCTCCAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGCGCCGAAAAAGTTGGCGGAAAACTGCTGTTCCATCTGCGCACGGCTGATGGTGGAAAGGGGGCCATACATGCCGAATCCGGCATTGTTAAAGATCCCATACAGACAATTATCGGTCAGGGCGATCACC  >  NC_000913/518488‑518913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑