Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	512,904	C→T	A110V (GCC→GTC)	ybaT →	putative transporter YbaT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	512,904	0	C	T	91.7%	25.7 / ‑5.3	12	A110V (GCC→GTC)	ybaT	putative transporter YbaT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (4/7);  minor base A (0/1);  total (4/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGCTCTCGTTACTGTACCTGTTGACGCTGGCGGTGAGCATCGCCATGGTCGCCCGTGCTTTTGGCGCTTATGCCGTGCAGTTTTTGCATGAAGGCAGCCAGGAGGAGCACCTTATTTTGCTCTACGCGTTGGGGATCATTGCGGTGATGACGCTTTTCAACTCCTTAAGCAACCATGCGGTAGGGCGGCTGGAAGTGATCCTCGTCGGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTC  >  NC_000913/512667‑513108                                                                                                                                                                                                                                              |                                                                                                                                                                                                             tCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGCTCTCGTTACTGTACCTGTTGACGCTGGCGGTGAGCATCGCCATGGTCGTCCGTGCTTTTgg                                                                                                                                                                                                  <  1:25188/250‑1 (MQ=255)    tCGCGCTGCTGGGGCAGGCTGCATTTCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTAGTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTGGGGGCGAGCTATCCCATCAATGGCGGCATTATCGTCGTCTTTCGTCTCGGATTAGGCAACGGCGTCTGTTCTCTGGCGCGCTCGGTGCTGGACCTGTTGACGCTGGCGGTGAGCATCGCCATGGTCGTCCGTGCTTTTGgcg                                                                                                                                                                                                <  1:337705/249‑1 (MQ=255)    tCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCGTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGCTCTCGTTACTGTACCTGTTGACGCTGGCGGTGAGCATCGCCATGGTCGTCCGTGCTTTTgg                                                                                                                                                                                                  <  1:234572/247‑1 (MQ=255)                                aaTGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGCTCTCGTTACTGTACCTGTTGACGCTGGCGGTGAGCATCGCCATGGTCGTCCGTGCTTGTGGCGCTTATGCCGTGCAGTTTTTGCATGAAgg                                                                                                                                                                    <  1:146221/249‑1 (MQ=255)                                                                                                gCCTATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGCTCTCGTTACTGTACCTGTTGACGCTGGCGGTGAGCATCGCCATGGTCGTCCGTGCTTTTGGCGCTTATGCCGGGCAGTTTTTGCATGAAGGCAGCCAGGAGGAGCACCTTATTTTGCTCTACGCGTTGGGGATCATTGCGGTGATGACGCTTTTCa                                                                                                   <  1:386409/250‑1 (MQ=255)                                                                                                  cTATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGAATTATCGACTTCTTTCGTCGCGGATTAGGAAACGGAGTATTTTCGCTGGCGCTCTCGTTACAGTACCTGTTGACGCTGGCGGTGAGCATCGCCATGGCCGTCCGTCCTTTTGGCGCTTATGCCGTGCAGCTTTTGTATGAAGGCAGCCAGGAGGAGCCCCTTATTTTGCTCAACGAGTTGGGGATCATTGAGGTGATGACg                                                                                                          >  1:396970/1‑241 (MQ=255)                                                                                                                             cAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGCTCTCGTTACTGTACCTGTTGACGCTGGCGGTGAGCATCGCCATGGTCGTCCGTGCTTTTGGCGCTTATGCCGTGCAGTTTTTGCATGAAGGCAGCCAGGAGGAGCACCTTATTTTGCTCTACGCGTTGGGGATCATTGCGGTGATGACGCTTTTc                                                                                                    <  1:128282/220‑1 (MQ=255)                                                                                                                                                                                             ntctcGTTACTGTACCTGTTGACGCTGGCGGTGAGCATCGCCATGGTCGTCCGTGCTTTTGGCGCTTATTCCGTGCCGATTTTGCATGGAGGCAGCCAGGAGGAGCACCTTATTTTGCTCTACGCGTTGGGGATCATTGCGGGGATGACGCTTTTCAACTCCTTAAGCAACCATGCGGTAGGGCGGCTGGGAGGGATCCTCGTCGGCATTAAAATGATGATACTGTTATTgattat                    >  1:358833/2‑231 (MQ=255)                                                                                                                                                                                                           ccTGTTGACGCTGGCGGTGAGCATCGCCATGGTCGTCCGTGCTTTTGGCGCTTATGCCGTGCAGTTTTTGCATGAAGGCAGCCAGGATGAGCACCGTATTTTGCTCTACGCGTTTGGGATCATTGCGGTGATGACGCTTTTCAACTCCTTAAGCAACCATGCGGTAGGGCGGCTGGAAGTGATCCTCGTTGTCATTAAAATGGTGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTc  <  1:207328/240‑1 (MQ=255)                                                                                                                                                                                                                         cGGTGAGCATCGCCATGGTCGACCGGGCGTTTGGCGCTTATTCCGTGCAGGTTTTGCATGAAGGCAGCCAGGAGTAGCACCTTATCTTGCGCTACGCGCTGGAGATCATTGCCGTGATGACGCTTTTCATCTCCTTAAGCAACCATGCGGTAGGGCGGCTGGAAGTGATc                                                          <  1:435459/170‑1 (MQ=255)                                                                                                                                                                                                                           gTGAGCATCGCCATGGTCGTCCGTGCTTTTGGCGCTTATGCCGTGCAGTTTTTGCATGAAGGCAGCCAGGAGGAGCACCTTATTTTGCTATACGCGTTGGGGCTCCTTGCGGTGATGACGCTTTTCAACTCCTTAAg                                                                                         >  1:212956/1‑137 (MQ=255)                                                                                                                                                                                                                                    gCCATGGTCGTCCGTGCTTTTGGCGCTTATGCCGTGCAGTTTTTGCATGAAGGCAGCCAGGAGGAGCACCTTATTTTGCTCTACGCGTTGGGGATCATTGCGGTGATGACGCTTTTCAACTCCTTAAGCAACCATGCGGTAGGGCGGCTGGAAGTGATCCTCGTCGGCATTAAAATGATGATCCTGTTATTGCTGATTATTGcc             >  1:17698/1‑204 (MQ=255)                                                                                                                                                                                                                                          gtcgtcCGTGCTTTTGGCGCTTATGCCGTGCAGTTTTTGCATGAAGGCAGCCAGGAGGAGCGCCTTATTTTg                                                                                                                                           <  1:99473/72‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                             TCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTGGGGGCGAGCTATCCCAGCAATGGCGGCATTATCGACTTCTTTCGTCGCGGATTAGGCAACGGCGTCTTTTCGCTGGCGCTCTCGTTACTGTACCTGTTGACGCTGGCGGTGAGCATCGCCATGGTCGCCCGTGCTTTTGGCGCTTATGCCGTGCAGTTTTTGCATGAAGGCAGCCAGGAGGAGCACCTTATTTTGCTCTACGCGTTGGGGATCATTGCGGTGATGACGCTTTTCAACTCCTTAAGCAACCATGCGGTAGGGCGGCTGGAAGTGATCCTCGTCGGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTC  >  NC_000913/512667‑513108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑