Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	511,602	G→A	intergenic (‑223/‑39)	copA ← / → glsA	Cu(+) exporting P‑type ATPase/glutaminase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	511,602	0	G	A	100.0%	24.2 / NA	12	intergenic (‑223/‑39)	copA/glsA	Cu(+) exporting P‑type ATPase/glutaminase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/3);  total (9/3)

GGTCGATAGTTTGTGACATAAAACACTCCTTTAAGACAGTTTTGACTGGCTGTGATAAAGGTTAAACCTTCCAGCAAGGGGAAGGTCAAGAAATTAATAAACCAGGCGGGTAAAAGTCCGTAAAGATTAAAAAATCGGCTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTAC  >  NC_000913/511361‑511820                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                           ggTCGATAGTTTGTGACATAAAACACTCCTTTAAGACAGTTTTGACTGGCTGTGATAAAGGATAAACCTTCCAGCAAGGGGAAGGTCAAGAAATTAATAAACCAGGCGGGTAAAAGTCCGTAAAGATTAAAAAATCGGCTCGATTTGCCTCAGGATTAGACATTTATCTCTTTGTTTTTCTGGAGTTAAGTTGCGGGTGCTAAGTTAAAACAGGATGCCTGAAAATCGGCACCGGGGGGAGAAATTACCTc                                                                                                                                                                                                                   >  1:217863/1‑251 (MQ=255)          gnnggnnnngnnnnnnnnccNNNAAGACANTNNTGACTNGCTNGGATAAAGGTTAAACCTTCCAGCAAGGGGAAGGTCAAGAAATTAATAAACCAGGCGGGTAAAAGTCCGTAAAGATTAAAAAATCGGCTCGATTTGCATCAGGATTNGACNTTTANCTNTTTGTTTTCCTGTAGNTAAGTTGCGTGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCNNCNNNGNTAGAAAtt                                                                                                                                                                                                                        <  2:311824/219‑1 (MQ=255)                                            gACTGGCTGTGATAAAGGTTAAACCTTCCAGCAAGGGGAAGGTCAAGAAATTAATAAACCAGGCGGGTAAAAGTCCGTAAAGATTAAAAAATCGGCTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGAAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGcaa                                                                                                                                                                         >  1:421497/1‑250 (MQ=255)                                                         aaaGGTTAAACCTTCCAGCAAGGGGAAGGTCAAGAAATTAATAAACCAGGCGGGTAAAAGTCCGTAAAGATTAAAAAATCGGCTCGATTTGCATCCGGAATAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGAAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAg                                                                                                                                                                               >  1:240912/1‑231 (MQ=255)                                                                      tCCAGCAAGGGGAAGGGCAAGAAATTAATAAACCAGGCGGGTAAA‑GTCCGTAAAGATTAAAAAATCGGCTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGTGCTAAGTTAAATCAGGAAGCCTGAAAATCGGCACCGGGGTGAGAAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCATACAAATTACAGCAGGCAGTGGATCAgg                                                                                                                                              <  1:146227/250‑1 (MQ=255)                                                                                           aaaTTAATAAACCAGGCGGGTAAAAGTCCGGAAAGATTAAACAATCGGCACCATTTGCCTCCGCATTCGTCCTTTTTCTCTTTGTTTTCCTCTAGTTTACTTGGGGGGGCTAAGTTAAATCATGATGCCTGGAAATCGGGCCCGGGGGGAGAAATTACATCCCGCATCTCTTAAAAGGAGTTAACAAAAAATGTTAGATTCAAAAAAATTAc                                                                                                                                                                >  1:167385/1‑212 (MQ=255)                                                                                                          gCGGGTAAAAGTCCGTAAAGATTATAAAATCGGCGCGAGTTGCATCATTATTAGACATGTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGAAATGACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCTTacac                                                                                                                                       <  1:178988/222‑1 (MQ=255)                                                                                                             ngTAAAAGTCCGTAAAGATTAAAAAATCGGCTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGAAATTACCTCCCGc                                                                                                                                                                                                               >  1:107409/2‑147 (MQ=255)                                                                                                                       ngTAAAGATTAAAAAATCGGCTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGCAAATCGGCACCGGGGTGAGAAATTACCTccc                                                                                                                                                                                                                 >  1:53571/2‑135 (MQ=255)                                                                                                                                          gCTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGAAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCTTACACCCAATTTCACTCACTTAAcgg                                                                                                                  >  1:127944/1‑211 (MQ=255)                                                                                                                                                  ngCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGAAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGACTGTACAAGGTc                                                                   >  1:91439/2‑250 (MQ=255)                                                                                                                                                                                                                                             gTGAGAAATTACCTCCCGCATCTATAAAAAGGAGTTAACAACAGATGTTAGATGCAAACAAATTACAGCCGGCAGTGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCACCGTCTATAGTGCGGGTGACAGTGATTAc  >  1:403106/1‑224 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                           GGTCGATAGTTTGTGACATAAAACACTCCTTTAAGACAGTTTTGACTGGCTGTGATAAAGGTTAAACCTTCCAGCAAGGGGAAGGTCAAGAAATTAATAAACCAGGCGGGTAAAAGTCCGTAAAGATTAAAAAATCGGCTCGATTTGCATCAGGATTAGACATTTATCTCTTTGTTTTCCTGTAGTTAAGTTGCGGGTGCTAAGTTAAATCAGGATGCCTGAAAATCGGCACCGGGGTGAGGAATTACCTCCCGCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGTACCAGGTCAACTGGCGGCAGTGGCTATCGTGACCTGCGATGGCAACGTCTATAGTGCGGGTGACAGTGATTAC  >  NC_000913/511361‑511820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑