Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	501,193	G→A	G357R (GGG→AGG)	gsk →	inosine/guanosine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	501,193	0	G	A	100.0%	39.2 / NA	17	G357R (GGG→AGG)	gsk	inosine/guanosine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/8);  total (9/8)

GGACTGGGTAGATCTGGTGCTGTGCACCGCCGGGCCAATCGGCTTGTATATGGCGGGCTTTACCGAAGACGAAGCGAAACGTAAAACCCAGCATCCGCTGCTGCCGGGCGCTATAGCGGAATTCAACCAGTATGAGTTTAGCCGCGCCATGCGCCACAAGGATTGCCAGAATCCGCTGCGTGTATATTCGCACATTGCGCCGTACATGGGCGGGCCGGAAAAAATCATGAACACTAATGGAGCGGGGGATGGCGCATTGGCAGCGTTGCTGCATGACATTACCGCCAACAGCTACCATCGTAGCAACGTACCAAACTCCAGCAAACATAAATTCACCTGGTTAACTTATTCATCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTT  >  NC_000913/500949‑501432                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                ggACTGGGTAGATCTGGTGCTGTGCACCGCCGGGCCAATCGGCTTGTATATGGCGGGCTTTACCGAAGACGAAGCGAAACGTAAAACCCAGCATCCGCTGCTGCCGGGCGCTATAGCGGAATTCAACCAGTATGAGTTTAGCCGCGCCATGCGCCACAAGGATTGCCAGAAGCCGCTGCGTGTATATTCGCACATTGCGCCGTACATGGGCGGGCCGGAAAAAATCATGAACACTAATGGAGCGAGGGAtn                                                                                                                                                                                                                                           <  1:270478/251‑2 (MQ=255)                                 ggCCAATCGGCTTGTATATGGCGGGCTTTACCGACGACGAAGCGAAACGCAAAACCCAACATCCGCTGCTGCCGGGCGATATAGCGGGATTCAACCAGTCTGAGGTTAGCCGCGCCATGCGCCACAAGGATTGCCAGAATCCGCTGCGTGTATATTCGCCCATTGCGCCGTACATGGGCGGGCCGGAAAAAATCATGAAAACTAATGGAGCGAGGGATGGGGCATTGGCAGCGTTGCTGCATGACATTAcc                                                                                                                                                                                                           >  1:382230/1‑251 (MQ=255)                                                        ggCTTTACCGAAGACGAAGCGAAACGTAAAACCCAGCATCCGATGCTGCCGGGCGCTATAGCGGAATTCAACCAGTATGAGTTTAGCCGCGCAATGCGCCACAAGGATTGCCAGAATCCGCTGCGTGTATATTCGCACATTGCGCCGTACATGGGCGGGCCGGAAAACATCATGAACACTAATGGAGCGAGGGATGGCGCATTGGCAGAGTTGCTGCATGACATTACCGCCACCAGCTACCATCGTAGCa                                                                                                                                                                                     >  1:75424/1‑250 (MQ=255)                                                                      cGAAGCGAAACGTAAAACCCAGCATCCGCTGCTGCCGGGAGATATAGCGGAATTCAACCAGTATGAGTTTAGCCGCGCCATGCGCCACAAGGATTGCCAGCATCCGCTGCGTGTATATTCGCACATTGCGCCGTACATGGGCGGGCCGGAAAAAATCATGAACACTAATGGAGCGAGGGATGGCGCATTGGCAGCGTTGCTGCATGACAt                                                                                                                                                                                                               >  1:302654/1‑210 (MQ=255)                                                                                   aaaaCCCAGCATCCGCTGCTGCCGGGCGCTATAGCGGAATTCAACCAGTATGCGTTTAGAAGCGCCATGCGCCCCAAGGATTGCCAGAATCCGCTGCGTGTATATTCGCACATTGCGCCGTACATGGGCGGGCCGGAAAAAATCATGAACACTAATGGAGCGAGGGATGGCGCATTGGCAGCGTTGCTGCATGACATTACCGCCAACAGCTCCCATCGTAGCAACGTACCAAACTCCAGCAAACATAAAtt                                                                                                                                                         >  1:255425/1‑251 (MQ=255)                                                                                          aGCATCCGCTGCTGCCGGGCGCTATAGCGGAATTCAACCAGTATGAGTTTAGCCGCGCCATGCGCCACAAGGATTGCCAGAATCCGCTGCGTGTATATTCGCACATTGCGCCGTACATGGGCGGGCCGGAAAAAATCATGAACACTAATGGAGCGAGGGATGGCGCATTGGCAGCGTTGCTGCATGACATTACCGCCAACAGCTACCATCGTAGCAACGTACCAAACTCCAGCAAACATAAATTCACCTgg                                                                                                                                                  <  1:252883/251‑1 (MQ=255)                                                                                                                                                          ccACAAGGATTTCCAGAATCCGCTGCGTGTATATTCGCACATTGCGCCGTACATGGGCGGTCCGGAAAAAATCATTAACACTAATGGAGCGAGGGATGGCGCATTGGCAGCGTTGCTGCATGACAt                                                                                                                                                                                                               <  1:429491/126‑1 (MQ=255)                                                                                                                                                                                             cGCACATTGCGCCGTACATGGGCGGGCCGGAAAAAATCATGAACACTAATGGAGCGAGGGATGGCGCATTGGCAGCGTTGCTGCATGACATTACCGCCAACAGCTACCATCGGAGCAACGTACCAAACTCCAGCAAACATAAATTCACCTGGTTAACTTATTCATCGTTAGCGCAg                                                                                                                          <  1:284080/176‑1 (MQ=255)                                                                                                                                                                                                          gTACATGGNNGGNNCNGNAAAAANCATGAACACTAATGGAGCGAGGGATGGCGCATTGGCAGCGtt                                                                                                                                                                                                                           >  2:298818/1‑66 (MQ=255)                                                                                                                                                                                                          gTACAGGGGCGGGCCGGAAAAAATCATGAACACTAATGGAGCGAGTGATGGCGCATTGGCAGCGtt                                                                                                                                                                                                                           <  1:298818/66‑1 (MQ=255)                                                                                                                                                                                                            aCATGGGCGGGCCGGAAAAAATCATGAACACTAATGGAGCGAGGGATGGCGCATTGGCAGCGTTGCTGCATGACATTACCGCCAACAGCTACCATCGTAGCAACGTACCAACCTCCAGCAAACATAAATTCCCCTGGTTAACTTATTCATCGTTAGCGCAgg                                                                                                                         >  1:72413/1‑162 (MQ=255)                                                                                                                                                                                                                 ggcgggcCGGAAAAAATCATGAACACTAATGGAGCGAGGGATGGCGCATTGGCAGCGTTGCTGCATGACATTACCGc                                                                                                                                                                                                         >  1:173310/1‑77 (MQ=255)                                                                                                                                                                                                                   cgggcCGGAAAAAATCATGAACACTAATGGAGCGAGGGATGGCGCATTGGCAGCGTTGCTGCATGACATTACCGCCAACAGCTACCATCGTAGCAACGTACCAAACTCCAGCAAACATAAATTCACCTGGTTAACTTATTCATCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAag                         <  1:368341/251‑1 (MQ=255)                                                                                                                                                                                                                           nanaanTCATGNACACNAATGGAGCGAGGGATNGCGCATTGGCAGCGTTGCTNNANGGCATNACCGc                                                                                                                                                                                                         <  2:173310/66‑1 (MQ=255)                                                                                                                                                                                                                              aaaTCATGAACACTAATGGAGCGAGGGATGGCGCATTGGCAGCGTTGCTGCATGACATTACCGCCAACAGCTACCATCGTAGCAACGTACCAAACTCCAGCAAACATAAATTCACCTGGTTAACTTATTCATCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTgg              <  1:154540/251‑1 (MQ=255)                                                                                                                                                                                                                                        cacTAATGGAGCGAGGGATGGCGCATTGGCAGCGTTGCTGCATGACATTACCGCCAACAGCTACCATCGTAGCAACGTACCAAACTCCAGCAAACATAAATTCACCTGGTTAACTTATTCATCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTa      >  1:329462/1‑249 (MQ=255)                                                                                                                                                                                                                                           tAATGGAGCGAGGGATGGCGCATTGGCAGCGTTGCTGCATGACATTACCGCCAACAGCTACCATCGTAGCAACGTACCAAACTACAGCAAACATAAATTCACCTGGTTAACTTATTCATCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGAACGTTAAGtt  >  1:153548/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                GGACTGGGTAGATCTGGTGCTGTGCACCGCCGGGCCAATCGGCTTGTATATGGCGGGCTTTACCGAAGACGAAGCGAAACGTAAAACCCAGCATCCGCTGCTGCCGGGCGCTATAGCGGAATTCAACCAGTATGAGTTTAGCCGCGCCATGCGCCACAAGGATTGCCAGAATCCGCTGCGTGTATATTCGCACATTGCGCCGTACATGGGCGGGCCGGAAAAAATCATGAACACTAATGGAGCGGGGGATGGCGCATTGGCAGCGTTGCTGCATGACATTACCGCCAACAGCTACCATCGTAGCAACGTACCAAACTCCAGCAAACATAAATTCACCTGGTTAACTTATTCATCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTT  >  NC_000913/500949‑501432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑