Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	500,069	C→T	intergenic (‑96/‑56)	aes ← / → gsk	acetylesterase/inosine/guanosine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	500,069	0	C	T	100.0%	38.2 / NA	15	intergenic (‑96/‑56)	aes/gsk	acetylesterase/inosine/guanosine kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/6);  total (9/6)

CAACGGTCTTCATTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAG  >  NC_000913/499913‑500292                                                                                                                                                             |                                                                                                                                                                                                                                caACGGTCTTCATTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTGCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGTTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTATATCCAAACATTACTTc                                                                                                                                   <  1:318726/251‑1 (MQ=255) caACGGGCTTCATTTCAGCAGAAATAAGGTCCATAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACTTCATTGTAAGGAACCACTTCCATGAAAATGCGATCCCGCCTGCTGATATTTAAACTGGCTGCGTCTCTCGCGTTCCCGTCAGATTGTGTTAACATTCGCCGCTCATTTAACCATCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTc                                                                                                                                   <  1:318743/251‑1 (MQ=255)                  gcagAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAACAACTCCTTTCAAATTACGTCATTGTCAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGTTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCACCGTAAacgca                                                                                                                       >  1:305706/1‑246 (MQ=255)                       aaTAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGTTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCgc                                                                                                             >  1:27040/1‑251 (MQ=255)                            ggTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTCAGGAACCCCTGCCCTGAAAATGCGATCCCGCCCGCTGATATTGAAACTAGCTGCGTCTCGCGCGTTCCCGTCAGATTGTGTTAACATTCGCCGCTCCGTTCACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTACCCCGTAAACGCACGCGATCCGCTGCtt                                                                                                        >  1:208730/1‑251 (MQ=255)                                                       ncTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGTTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACg                                                                                  >  1:269153/2‑246 (MQ=255)                                                               cAAAAACTCCTTTCAAATTACGTCATGGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGTTCCCGTCAGAt                                                                                                                                                                                                                      <  1:393324/106‑1 (MQ=255)                                                                  aaaCTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTACTGATATTGAAACTGGCTGCGTCTCGCGCGTTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTg                                                                  <  1:367566/251‑1 (MQ=255)                                                                        cTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGACTGCTGATATTGAAACTGGATGCGTCTCGCGCGTTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTCACCACCCGTAAAAACAACCATGAAATCTccc                                                                                                                                                              >  1:449517/1‑153 (MQ=255)                                                                                                       gCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGTTCCCGTCAGATtg                                                                                                                                                                                                                    >  1:125020/1‑68 (MQ=255)                                                                                                                         ngCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGTTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGAtgatga            >  1:110148/2‑250 (MQ=255)                                                                                                                         cGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGTTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCg                          <  1:439128/236‑1 (MQ=255)                                                                                                                                   tatTGAAACTGGCTGCGTCTCGCGCGTGCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTGCCCGGTAAACTTAAATCCAAACATTGCTTCCCCGGAAACGCACGCGGTCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCATTGCTTCCTGTGGAGTTGGTATCGATCAAACGCTGGCCGATATTGAAGCGAAAGAGGATGATGAATTTATTGAg  <  1:298153/250‑1 (MQ=255)                                                                                                                                    atTGAAACTGGCTGCGTCTCGCGCGTTCCCGTCAGATTGTGTTAACATTCGCTGCTCAGTTAACAAACTGTAAAaacacc                                                                                                                                                                           >  1:212007/1‑78 (MQ=255)                                                                                                                                                  ngTCTCGCGCGTTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAAc                                                                                   >  1:108528/2‑154 (MQ=255)                                                                                                                                                             |                                                                                                                                                                                                                                CAACGGTCTTCATTTCAGCAGAAATAAGGTCCAGAACAGGTAGTTTGTTTTCCGGCTTCATACAAAAACTCCTTTCAAATTACGTCATTGTAAGGAACCACTGCCATGAAAATGCGATCCCGCCTGCTGATATTGAAACTGGCTGCGTCTCGCGCGCTCCCGTCAGATTGTGTTAACATTCGCCGCTCAGTTAACCACCCGTAAAAACAACCATGAAATTTCCCGGTAAACGTAAATCCAAACATTACTTCCCCGTAAACGCACGCGATCCGCTGCTTCAGCAATTCCAGCCAGAAAACGAAACCAGCGCTGCCTGGGTAGTGGGTATCGATCAAACGCTGGTCGATATTGAAGCGAAAGTGGATGATGAATTTATTGAG  >  NC_000913/499913‑500292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑