Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	492,215	C→T	L42F (CTT→TTT)  L42F (CTT→TTT)	dnaX → dnaX →	DNA polymerase III subunit tau DNA polymerase III subunit tau

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	492,215	0	C	T	100.0%	29.9 / NA	13	L42F (CTT→TTT)  L42F (CTT→TTT)	dnaX dnaX	DNA polymerase III subunit tau DNA polymerase III subunit tau
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/7);  total (6/7)

GATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTG‑GCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAATCGACGCCGCCTCGCGCACCAAAGTTGAAGATACCCGCGACCTGCTGGATAACGTCCAGTACGCTCCGGCGCG  >  NC_000913/492005‑492441                                                                                                                                                                                                                   |                                                                                                                                                                                                                                    gATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATTTTTTTTCCGGCACCCGTG‑GCGTCGGAAAAACCTCTATCGc                                                                                                                                                                                            >  1:385387/1‑251 (MQ=255)                   ncGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATTTTTTTTCCGGCACCCGTG‑GCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAggg                                                                                                                                                                          >  1:116855/2‑251 (MQ=255)                                    tgtTAGCATTAACCCTTCGTGCATCGACCTTCCAGCGGTTCAGAGCCTGCCAATGAGGTATCAGGGCTTAGCCCGAAAAAGACGGCCACAAACCTTTGCTGACGACGTCGGCCCGGAACCAGTGCTGACCGCACTGGCGAACGGATTGAAGTTAGGGCGAAATCATCATGCTTATTTTTTTTCCGGCACCCGTG‑GCGTCGGAAAAACCTCTATCGCCCGACTGTTGGCGAAGGGGCTAAACTGCGAAACCg                                                                                                                                                         >  1:257755/1‑251 (MQ=255)                                              tccccTTCGTGAATCCACCTTCCAGCGTTTCAGATCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCTGCCAGCAACATGTGCTGACCGCACTGGCGAACGGCGTGTCGTTAGGGCGTATTCTTCTTGCTTATTTTTTTTCCGGCACCC‑TGTGCGTCTGAAAAACCTCTATCGCCCGACTTCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACcg                                                                                                                                                <  1:56925/249‑1 (MQ=255)                                                                                                         aGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTGATTTTTTTTCCGGCACCCGTG‑GCGGCGGAAAAACCTCTATCGCCCTACTGCTGTCGAAGGGGCTAAACTGCTAAATCTGCATTACCGCGACGCCGGGCGGCGTGTTCGGTAACAGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATc                                                                                    <  1:390624/251‑1 (MQ=255)                                                                                                           cccGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATTTTTTTTCCGGCACCCGTG‑GCGTCGGAAAAACCTCTATCGCCCGTCTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTg                                                                                  >  1:445322/1‑251 (MQ=255)                                                                                                                       gcCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATTTTTTTTCCGGCAc                                                                                                                                                                                                                        <  1:342131/106‑1 (MQ=255)                                                                                                                                   ttGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCATATTTTTTTTCCGGCACCCGTG‑GCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGTGCGCTTTGTCGATCTGATTGAAATCGACGCCGCCTcgc                                                            <  1:212934/249‑1 (MQ=255)                                                                                                                                    nnnnnncgnnnnNGGCNAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATTTTTTTTCCGGCACCCGTG‑GCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGa                                                                                                                                                              <  2:275965/144‑1 (MQ=255)                                                                                                                                              gtcgGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATTTTTTTTCCGGCACCCGTG‑GCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTaanatgnnnnntcnn                                                                                                                                                        >  2:265546/1‑133 (MQ=255)                                                                                                                                              gtcgGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATTTTTTTTCCGGCACCCGTG‑GCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGcn                                                                                                                                                               <  1:265546/139‑2 (MQ=255)                                                                                                                                                                      gCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATTTTTTTTCCGGCACCCGTG‑GCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAATCGACGCCGCCTCGCGCACCAAAGTTGAAGATACCCGCGACCTGCTGGATa                        >  1:154711/1‑250 (MQ=255)                                                                                                                                                                                            tAGGGCGTATTCATCATGCTTATTTTTTTTCCGGCACCCGTG‑GCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGTGCGCTTTGTCGAGCTGATTGAAATCGACGCCGCCTCACGCACCAAAGTTGAAGATACCCGCGACCTGCTGGATAACGTCCAGTACGCTCCGGcgcg  <  1:296360/250‑1 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                                                                                    GATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTG‑GCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAATCGACGCCGCCTCGCGCACCAAAGTTGAAGATACCCGCGACCTGCTGGATAACGTCCAGTACGCTCCGGCGCG  >  NC_000913/492005‑492441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑