Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	480,686	C→T	R8K (AGA→AAA)	tomB ←	Hha toxicity modulator TomB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	480,686	0	C	T	100.0%	59.2 / NA	21	R8K (AGA→AAA)	tomB	Hha toxicity modulator TomB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/12);  total (9/12)

ACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCTGTGCGATATCATGTCTTTTGGGTGAGTATTCATCCATAACGCGTCCCCTTCTTAGCGGTTGAACTAACGGACACCTTTCGGGATGGAAAAAACTTACTGACCTGGACTTGCCCTCTCGTTTGTTAGCTTAACTATAAGCCACTCTTTGCAGGTTTTCATCGCATTATTACGAAAAATTACAATTAGGAAGGAAGCGGGACAGGACTCACTTTTTCATACTAGCACTAACCCGCAGCAGGTAAAAGC  >  NC_000913/480445‑480917                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                        actGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCTGTGCGATATCATGTTTTTTGGGNg                                                                                                                                                                                                                                <  1:347072/251‑1 (MQ=255)                        ccAGATATTAGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCTGTGCGATATCATGTTTTTTGGGTgtgatttcttcattacggcgcccc                                                                                                                                                                                                         >  1:103132/1‑228 (MQ=255)                         cAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATCCTTAATTTTTTAATTTATTGCTAAGTTCGCAATATTCTCAATCAGTTCATTCAACTGGAGGGTGATCTCCTAGTTTGGTTCGTTTGCCCAGCCATGATTGCTTGCTTCAAGGTTTGCAAGGCAGTCATGAGACATGGTTTCTCAGAGAATCGTAATCTGTGCGATATCATTTTTTTTGGGTGAGCATTCATCCa                                                                                                                                                                                                                    <  1:63281/239‑1 (MQ=255)                                                                               gTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCTAGGTTGGGTCGTTTTCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCTGTGCTATATCATGTTTTTTGGGTGAGTATTCATCCATAACGCGTCCCCTTCTTAGCGGTTGAGCTAACGGACACCTTTCGGGATGGAAAAAACTTACTGAc                                                                                                                                                   <  1:427146/250‑1 (MQ=255)                                                                               gTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCTGTGCGATATCATGTTTTTTGGGTGAGTATTCATCCATAACGCGTCCCCTTCTTAGCGGTTGAACTAACGGACACCTTTCGGGATGGAAAAAACTTACTGAcc                                                                                                                                                  >  1:189013/1‑251 (MQ=255)                                                                                                          ntCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGCGAAACTTAAGCTGTGCGATATCATGTTTTTTGGGTGAGTATTCATCCATAACGCGTCCCCTTCTTAGCGGTTGAACTAACGGACACCTTTCGGGATGGAAAAAACTTACTGACCTGGACTTGCCCTCTCGTTTGTTAGcat                                                                                                                       >  1:49502/2‑249 (MQ=255)                                                                                                                                 ggTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCACGGTTTGCAAGGCAGTCATGATAAAGGGTTTCACAGAGAAACTTAAGCTGTGCGATATCATGTTTTTTGGGTGAGTATTCATCCATAACGCGTCCCCTTCTTAGCGGTTGAACTAACGGACACCt                                                                                                                                                                            >  1:216141/1‑175 (MQ=255)                                                                                                                                        cGCCGAGGTTGGGTCGTGTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCTGTGCGATATCATGTTTTTTTGGTGAGTATTCATCCATAACGCGTCCCCTTCTGAGCGGTTGAACTAACGGACACCTTTCGGGATGGAAAAAACTTACTGACCTGGan                                                                                                                                             <  1:93460/199‑2 (MQ=255)                                                                                                                                          ccGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGATGTGCGATATCATGTTTTTTGGGTGAGTATTCATCCATAACGCGTCCCCTTCTTAGCGGATGAACTAACGGACACCTTTCGGGATGGAAAAAACTTACTGACCTGGACTTGCCCTCTCGTTTGTTAGCTTAACTATAAGCCACTCTTTGCAGGTTTTCATCg                                                                                       >  1:251628/1‑251 (MQ=255)                                                                                                                                                    gTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCTGTGCGATATCATGTTTTTTGGGTGAGTATTCATCCATAACGCGTCCCCTTCTTAGCGGTTGAACTAACGGACACCTTTCGGGATGGAAAAAACTTACTGACCTGGACTTGCCCTCTCGTTTGTTAGCTTAACTATAAGCCACTCTTTGCAGGTTTTCATCGCATTATTACg                                                                             <  1:15689/251‑1 (MQ=255)                                                                                                                                                      cGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCTGTGCGATATCATGTTTTTTGGGTGAGGATTCATCCATAACGCGTCCCCTTCTTAGCGGTTGAACTAACGGACACCTTTCGGGATGGAAAAAACTTACTGACCTGGACTTGCCCGCTCGTTTGTTAGCTTAACTATAAGCCGCTCTTTGCAGGTTTTCATCGCa                                                                                     <  1:418688/241‑1 (MQ=255)                                                                                                                                                           aCCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCTGTGCGATATCATGTTTTTTGGGTGAGTATTCATCCATAACGCGTCCCCTTCTTAGCGGTTNAACNNNNNGannnnnn                                                                                                                                                                           >  2:270178/1‑144 (MQ=255)                                                                                                                                                                                 cAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCTGTGCGATATCATGTTTTTTGGGTTAGTATTCATCCATAACGCGTCCCCTTCGTAGCGGTTGAACTAACGGACACCTTTCGGGATGGAAAAAACTTACTGACCTGGACTTGCCCTCTCGTTTTTTAGCTTAACTATAAGCCACTCTTTGCAGTTTTTCATCGCATTATTACGAAAAATTACAATTAGGAAGGAAGCGGGAc                                                <  1:221541/251‑1 (MQ=255)                                                                                                                                                                                 cAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCTGTGCGATATCATGTTTTTTGGGTGAGTATTCATCCATAACGCGTCCCCTTCTTAGCGGTTGAACTAACGGACACCTTTCGGGATGGAAAAAACTTACTGACCTGGACTTGCCCTCTCGTTTGTTAGCTTAACTATAAGCCACTCTTTGCAGGTTTTCATCGCATTATTACGAAAAATTACAATTAGGAAGGAAGCGGGan                                                <  1:270178/251‑2 (MQ=255)                                                                                                                                                                                        tGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCTGTGCGATATCATGTTTTTTGGGTGAGTATTCATCCATAACTCTGCCCCTTCTTAGCGGTTGAACTAACGGACACCTTTCGGGATGGAAAAAACTTAcn                                                                                                                                                      <  1:90488/142‑2 (MQ=255)                                                                                                                                                                                            aGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCTGTGCGATATCATGTTTTTTGGGTGAGTATTCATCCATAACGCGTCCCCTTCTTAGCGGTTGAACTAACGGACACCTTTCGGGATGGAAAAAACTTACTGACCTGGACTTGCCCTCTCGTTTGTTAGCTTAACTATAAGCCACTCTTTGCAGGTTTTc                                                                                           <  1:303097/197‑1 (MQ=255)                                                                                                                                                                                                      gATACAGGGTTTCACAGAGAAACTTAAGCTGTGCGATATCATGTTTTTTGGGTGAGTATTCATCCATAACGCGTCCCCTTCTTAGCGGTTGAACTAACGGACACCTTTCGGGATGGAAAAAACTTACTGACCTGGACTTg                                                                                                                                          >  1:4524/1‑140 (MQ=255)                                                                                                                                                                                                                              nanacNGTGCGNTATCNTGTTTTTTGGGTGAGNATTCATCCATAACGCGTCCNNTNCTTAGNGGTTg                                                                                                                                                                                           <  2:169833/63‑1 (MQ=255)                                                                                                                                                                                                                               aaGCTGTGCGATATCATGTTTTTTGGGTGAGTATTCATCCATAACGCGTCCCCTTCGTAGCGGTTGAACTAACGGACACCTTTCGGGAGGGAAATAACTTACTGACCTGGACTTGCCCTCTCGTTTGTTAGCGTAACTATAAGCCATTCTTTGCAGTTTTTCATCGCATGATTACGAAAAATTACAATTAGGAACGAAGCGTGACAGGACTCACTTTTTCATACTAGCACTAACCCGCAGCAGGTAAAAGc  <  1:418166/251‑1 (MQ=255)                                                                                                                                                                                                                                 gCTGTGCGATATCATGTTTTTTGGGTGAGTATTCATCCATAACGCGTCCCCTTCTTAGCGGTTGAACTAACGGCCACCTTTCGGGATGGAAAACACTTACTGACCTgg                                                                                                                                               >  1:229765/1‑108 (MQ=255)                                                                                                                                                                                                                                      gCGATATCATGTTTTTTGGGTGAGTATTCATCCATAACGCGTCCCCTTATTAGCGGTTGAACTACTGGACACCTTTAGGGCTGGAAAAAACTTACTGACCTGGACTTGCAATCTCGTTTGTAAGCTTACCTATAAGCCACTCTTTGCAGGTTTTCATCGCATTATTACGAAAAATTACAATTAGGAAGGAAGCGGGACAGGAc                                           >  1:407384/1‑203 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                        ACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAGGTCGCAATATGCTCAATCAGTTCATTCAACTGGAGGTTGATCGCCGAGGTTGGGTCGTTTACCCAGCCATGATTGCTTTCTTCAAGGTTTGCAAGGCAGTCATGATACAGGGTTTCACAGAGAAACTTAAGCTGTGCGATATCATGTCTTTTGGGTGAGTATTCATCCATAACGCGTCCCCTTCTTAGCGGTTGAACTAACGGACACCTTTCGGGATGGAAAAAACTTACTGACCTGGACTTGCCCTCTCGTTTGTTAGCTTAACTATAAGCCACTCTTTGCAGGTTTTCATCGCATTATTACGAAAAATTACAATTAGGAAGGAAGCGGGACAGGACTCACTTTTTCATACTAGCACTAACCCGCAGCAGGTAAAAGC  >  NC_000913/480445‑480917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑