Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	472,585	C→T	intergenic (+168/‑13)	mdlB → / → glnK	ABC transporter family protein MdlB/nitrogen regulatory protein PII‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	472,585	0	C	T	100.0%	38.2 / NA	17	intergenic (+168/‑13)	mdlB/glnK	ABC transporter family protein MdlB/nitrogen regulatory protein PII‑2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/12);  total (5/12)

CGGGCGAAGAGCTGGCAGCCAGCGTGCGTGAAGAGGAATCATTGAGCGCCTGAATAGCGCAATATTTCATCGTTGGTGCAAAAATGTAACGCACTGTGCACTGTCATAGTGCGTTTTCATTTTCAAACTTCTTAACTTCCTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGAC  >  NC_000913/472365‑472801                                                                                                                                                                                                                             |                                                                                                                                                                                                                         cGGGCGAAGAGCTGGCAGCCAGCGTGCGTGAAGAGGAATCATTGAGCGCCTGAATAGCGCAATATTTCATCGTTGGTGCAAAAATGTAACGCACTGTGCACTGTCATAGTGCGTTTTCATTTTCAAACTTCTTAACTTCCTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGATCGGAGGGGATCTATGAAGCTGGTGACCGtn                                                                                                                                                                                            <  1:130475/251‑2 (MQ=255)           gCTGGCAGCCAGCGTGCGTGAAGAGGAATCATTGAGCGCCTGAATAGCGCAATATTTCATCGTTGGTGCCAACCTGCAACGCACCGTGCTCTGTCATAGTGCGTTTTAATTTTCAAACTTCTTAACTTCCTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGATCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAAc                                                                                                                                                                                  >  1:238296/1‑251 (MQ=255)              ggCAGCCAGCGTGCGTGAAGAGGAATCATTGAGCGACTGAATAGCGCAATATTTCATCGGTGGTGCAAAAATGTAACGCACTGTGCACTGTCATAGTGCGTTTTCATTTTCAAACTTCTTAACTTCCTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGGATTCTGATCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCAt                                                                                                                                                                               <  1:173370/251‑1 (MQ=255)                cagccagcGTGCGTGAAGAGGAATCATTGAGCGCCTGAATAGCGCAATATTTCATCGTTGGTGCAAAAATGTAACGCACTGTGCACTGTCATAGTGCGTTTTCATTTTCAAACTTCTTAACTTCCTGCTCTCTTTCTCGTTTTGCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGATCGGAGTGGATCTATGAAGc                                                                                                                                                                                                       <  1:341822/225‑1 (MQ=255)                                                    gAATAGCGCAATATTTCATCGTTGGTGCAAAAATGTAACGCACTGTGCACTGTCATAGTGCGTTTTCATTTTCAAACTTCTTAACTTCCTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATGTCCGAATTCTGATCGGAGGGGATCGATGAAGCTGGTGACCGTTATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCa                                                                                                                                           <  1:244913/249‑1 (MQ=255)                                                          cgcAATATTTCATCGTTGGTGCAAAAATGTAACGCACTGTGCACTGTCATAGTGCGTTTTCATTTTCAAACTTCTTAACTTACTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGATCGGAGGGGATCTATGAAGCTGGTGACCGTGAt                                                                                                                                                                                          >  1:366446/1‑196 (MQ=255)                                                                atTTCATCGTTGGTGCAAAAATGTAACGCACTGTGCACTGTCATAGTGCGTTTTCATTTTCAAACTTCTTAACTTCCTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGATCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCGGGGc                                                                                                                             <  1:19751/251‑1 (MQ=255)                                                                       cGTTGGTGCAAAAATGTAACGCACTGTGCACTGTCATAGTGCGTTTTCATTTTCAAACTTCTTAACTTCCTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGATCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCg                                                                                                                      <  1:177308/251‑1 (MQ=255)                                                                           ggTGCAAAAATGTAACGCACTGTGCACTGTCATAGTGCGTTTTCATTTTCAAACTTCTTAACTTCCTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGATCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCAGTCAAGCTGGAAGACGTTCGTGAAGCGTTAGCTTCCATTGGTATTCAGGGCCTGACCGTCAc                                                                                                                  <  1:363177/251‑1 (MQ=255)                                                                                                          aTAGTGCGTTTTCATTTTCAAACTTCTTAACTTCCTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGATCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAggg                                                                                   <  1:126919/251‑1 (MQ=255)                                                                                                          aTAGTGCGTTTTCATTTTCAAACTTCGTAACTTCCTGCTCTCTTACTCGTTTTTCATTTCTGGCACAACGCTTGCAATAACCTCTTCGTGCAGCAGAACCAATACCGAATTCTGATCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGACGACGTTCGTGAAGCGGTATCTTACATTGGCATTAAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTAAGCAAggg                                                                                   >  1:381050/1‑251 (MQ=255)                                                                                                                                     tAACTTCCTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGATCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGGCAGAAAGGGCATGCCTAGCTGTACCGGGGGGCgn                                                          <  1:131384/249‑2 (MQ=255)                                                                                                                                              gCTCTCTTTCTCGTTTTTCATTTCTGGCCCACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGATTTCTGATCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATctaa                                                                                                                                                                                   >  1:140776/1‑116 (MQ=255)                                                                                                                                                                     cTGGCACACCGATTGCAATACCTTCTTAGTGTAGCAGAACCATTACCGAATTATGATCGG‑CGGGATCTATGAAGCTGGTGACCGTGATAATCCCACCATTCAAGCTGGAAGACGCTCGTGAAGCGTTATCTTCCATTGGACTTCAGGGCCTGACCGTCACCGCAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCACAAGTAAAAAtt                       >  1:143259/1‑251 (MQ=255)                                                                                                                                                                              cGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGATCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGGTATCTTCCAGTGGTATGCAGGGCGTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTTAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGc               <  1:248869/251‑1 (MQ=255)                                                                                                                                                                                            tcttcGTGTAGCAGAACCATTACCGAATTCTGATCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGTGCGTCAGAAAGGGCATGCCGAGCGGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGAc  <  1:72701/250‑1 (MQ=255)                                                                                                                                                                                                                       ttCTGATCGGAGGGGATCTATGAAGCTGGTGACCGGGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCAcn                                                                                                                 <  1:46880/112‑2 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                         CGGGCGAAGAGCTGGCAGCCAGCGTGCGTGAAGAGGAATCATTGAGCGCCTGAATAGCGCAATATTTCATCGTTGGTGCAAAAATGTAACGCACTGTGCACTGTCATAGTGCGTTTTCATTTTCAAACTTCTTAACTTCCTGCTCTCTTTCTCGTTTTTCATTTCTGGCACACCGCTTGCAATACCTTCTTCGTGTAGCAGAACCATTACCGAATTCTGACCGGAGGGGATCTATGAAGCTGGTGACCGTGATAATCAAACCATTCAAGCTGGAAGACGTTCGTGAAGCGTTATCTTCCATTGGTATTCAGGGCCTGACCGTCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGAC  >  NC_000913/472365‑472801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑