Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 456,645 C→T intergenic (+214/‑32) tig → / → clpP trigger factor/ATP‑dependent Clp protease proteolytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913456,6450CT94.1% 39.4 / NA 17intergenic (+214/‑32)tig/clpPtrigger factor/ATP‑dependent Clp protease proteolytic subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/7);  minor base A (1/0);  total (10/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAG‑CCTCTTTCGGTGTTAGCGTAACAACAAAAGATT‑GTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGG‑AGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACCCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGCGTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCAGAAA  >  NC_000913/456422‑456887
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   
gcagGCGTAATTTACGCAGCATAACGCGCTAAATTCGCCCAAAGGCCCGTCACCGCAAGGCGGTGGGCTTTTTTTTGACATGCATTTTACATGGAAACGTGCGAAAAG‑CCTCTTTCGGTGTTAGAGTAACAACAAAAGATT‑GTTATGCTTGAAATATGGTGATGCCGTACCCACAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACACTCGGTACAGAAGGTTTTTTCAATTTTATCCAGG‑AGAc                                                                                                                                                                                                                         >  1:422265/1‑251 (MQ=255)
        aaTTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTCGGCTTTTTTTT‑TCATTGATTTTGGTTTGAACAAGGCGAAAAGGCAACTTT‑GGTGGTAGCCCAAACACAAAAGATTAGTAATGCT‑GAAATATGGGGGTTCCGGACCCATAAAACAAGGACTTGGTGAAAATCCGTTCCTAAGGTTACAAATGGTACAGTAGGGTTTTTCCATTTTATCCAGGGAGACG‑AAATgac                                                                                                                                                                                                                >  1:229629/1‑249 (MQ=255)
              cGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGCGGGCTTTTTTTTGTCATGACTTTTACATGGAACCGTGCGAAAAG‑CCTCTTTCGGTGTTAGCGTCACAACAAAAGATT‑GTTATGCTTGACATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGTAGGTTTTTTCAATTTTATCCAGG‑AGACGGAAATGACATAc                                                                                                                                                                                                            >  1:221260/1‑250 (MQ=255)
                                    ncacaAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAG‑CCTCTTTCGGTGTTAGCGTAACAACAAAAGATT‑GTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGTAGGTTTTTTCAATTTTATCCAGG‑AGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTg                                                                                                                                                                                      >  1:86626/2‑250 (MQ=255)
                                             cccGTCAACGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAG‑ACTCTTTCGGTGTTAGCGTCACAACAAAAGATT‑GTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAAGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTAAAGTAGGTTTTTTCAATTTTATCCAGG‑AGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACCCCatat                                                                                                                                                                            >  1:60742/1‑251 (MQ=255)
                                                       ccAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAG‑CCTCTTTCGGTGTTAGCGTAACAACAAAAGATT‑GTTCTGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGTAGGTTTTTTCAATTTTATCCAGG‑AGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACCACATATGGCGCTGGTg                                                                                                                                                                  >  1:417949/1‑251 (MQ=255)
                                                              gtggGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAG‑CCTCTTTCGGTGTTAGCGTAACAACAAAAGATT‑GTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGTAGGTTTTTTCAATTTTATCCAGG‑AGACGGAAATGTCATACAgn                                                                                                                                                                                                         <  1:113306/205‑2 (MQ=255)
                                                                                         cATGGAACCGTGCGAAAAG‑CCTCTTTCGGTGTTAGCGTAACAACAAAAGATT‑GTTATGCTTGAAATATGGTGATGCCCTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGTAGGTTTTTTCAATTTTATCCAGG‑AGACGGAAATGGCATACAGCGGCTAACGATATATCTTTGCCCCCCATATGGCGCTGGTGCCGATGGTCACTGAACAGACCTCACGCGGTGAgc                                                                                                                                <  1:190756/251‑1 (MQ=255)
                                                                                            ggAACCGTGCGAAAAG‑CCTCTTTCGGTGTTAGCGTAACAACAAAAGATT‑GTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGTAGGTTTTTTCAATTTTATCCAGG‑AGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACCCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAgcgc                                                                                                                              <  1:123738/250‑1 (MQ=255)
                                                                                                                                     caaAAGATT‑GTTATGCTTGAAATATGGTGATGCCGTAACCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTCCAGTAGGTTTTTTCAATTTTATCCAGG‑AGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACCCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGCGTCAt                                                                                     >  1:383882/1‑251 (MQ=255)
                                                                                                                                                                 tGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGTAGGTTTTTTCAATTTTATCCAGG‑AGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACCCCNTATNNNNNTg                                                                                                                                                                     >  2:271431/1‑144 (MQ=255)
                                                                                                                                                                 tGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGTAGGTTTTTTCAATTTTATCCAGG‑AGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACCCCATATGGCGCTGGTGCCGATGGTCATTGAACAGan                                                                                                                                              <  1:271431/167‑2 (MQ=255)
                                                                                                                                                                                    catggACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGTAGGTTGTTTCAATGTTATCCAGG‑AGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTg                                                                                                                                                                                      <  1:236511/105‑1 (MQ=255)
                                                                                                                                                                                                 aTAATCCGTCCATAAGGTTACAAGCGGTACAGTAGGGTTTTTCAATTTTATCCAGG‑AGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCAcccc                                                                                                                                                                                <  1:293579/101‑1 (MQ=255)
                                                                                                                                                                                                           cATAAGGCTACAATCGGTACAGTAGGTTTTTTCAATTTTATCCAGG‑AGACGGACATGTCATACAGCGGCGAACGAGATAACTTTGCACCCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGCGTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGc                >  1:143343/1‑251 (MQ=255)
                                                                                                                                                                                                                  ttACAATCGGTACAGTAGGTTTTTTCAATTTTATCCAGG‑AGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACCCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGCGTCATTTTTCTGACTGGCCAgg                                                                    <  1:10508/192‑1 (MQ=255)
                                                                                                                                                                                                                          ngTACAGTAGGTTTTTTCAATTTTATCCAGG‑AGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACCCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGCGTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCAGaaa  >  1:280464/2‑250 (MQ=255)
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   
GCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAG‑CCTCTTTCGGTGTTAGCGTAACAACAAAAGATT‑GTTATGCTTGAAATATGGTGATGCCGTACCCATAACACAGGGACTAGCTGATAATCCGTCCATAAGGTTACAATCGGTACAGCAGGTTTTTTCAATTTTATCCAGG‑AGACGGAAATGTCATACAGCGGCGAACGAGATAACTTTGCACCCCATATGGCGCTGGTGCCGATGGTCATTGAACAGACCTCACGCGGTGAGCGCTCTTTTGATATCTATTCTCGTCTACTTAAGGAACGCGTCATTTTTCTGACTGGCCAGGTTGAAGACCACATGGCTAACCTGATTGTGGCGCAGATGCTGTTCCTGGAAGCGGAAAACCCAGAAA  >  NC_000913/456422‑456887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑