Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	446,642	C→T	G9S (GGT→AGT)	yajR ←	putative transport protein YajR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	446,642	0	C	T	100.0%	59.9 / NA	22	G9S (GGT→AGT)	yajR	putative transport protein YajR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/8);  total (14/8)

AATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTCAGAACCGGCAGAACCATAAACATGCCCAGCATGCGCAACGAGAATACGGTCCCTAAACCCCAGGTCGCGCGCCTCTCACCTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGG  >  NC_000913/446469‑446884                                                                                                                                                                              |                                                                                                                                                                                                                                                   aaTCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTCAGAACCGGCAGAACCATAAACATGCCCAGCATGCGCAACGAGAATACGGTCCCTAAACCCCAGGTCGCGCGCCTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCTACTAGTGTAAATCGTCAAACGAATGGGGt                                                                                                                                                                       <  1:125215/251‑1 (MQ=255)                        gACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAACCCTGCCGTACGTGGTAAGACCCGGCAGAACCATACACATGCCCAGCATGCGCAACGAGAGTACGGTCCCTAAACCCCCGGTCGCGCGCCTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTa                                                                                                                                                                                           >  1:428240/1‑208 (MQ=255)                                     aaTGGCAATACCGATTAATGCTTCGCTGGCCCCTTGCAGAGCCATGCCGTACGTGGTCAGAACCGGCAGAACCATAAACATGCCCAGCATGCGCAACGAGAATACGGTCCCTAAACCCCAGGTCGCGCGCCTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGTGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAggg                                                                                                                                   <  1:148725/251‑1 (MQ=255)                                               ccGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTCAGAACCGGCAGAACCATAAACGTGCCCAGCATGCGCAACGAGAATACGGTCCCTAAACCCCAGGTCGCGCGCCTCTCACTTGGCGTCATTTTATAATCGTTCa                                                                                                                                                                                                                             >  1:235917/1‑151 (MQ=255)                                                                                                       cAGAACCATAAACATGCCCAGCATGCGCAACGAGAAAACGGTCCCTAAACCCCAGGTCGCGCGCCTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACg                                                                                                                                                                               <  1:18527/141‑1 (MQ=255)                                                                                                           aCCATAAACATGCCCAGCATGCGCAACGAGAATACGGTCCCTAAACCCCAGGTCGCGCGCCTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATAGTTCTAGAGCGATATGTTTCTAATGCTAAGAAAAAAGGTGTCGTATTACCTTTCTAATAGATAGTATTTGCTACCACAcag                                                             <  1:156096/251‑1 (MQ=255)                                                                                                                aaaCATGCCCAGCATGCGCAACGAGAATACGGTCCCTAAACCCCAGGTCGCGCGCCTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagcagcc                                                        <  1:361580/251‑1 (MQ=255)                                                                                                                 aaCATGCCCAGCATGCGCAACGAGAATACGGTCCCTAAACCCCAGGTCGCGCGCCTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagcagcc                                                        >  1:217911/1‑250 (MQ=255)                                                                                                                       cncAGCATGCGCAACGAGAATACGGTCCCTAAACCCCAGGTCGCGCGCCTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCgaga                                                                                                                                      >  1:349391/1‑166 (MQ=255)                                                                                                                        ccAGCATGCGCAACGAGAATACGGTCCCTAAACCCCAGGTCGCGCGCCTCTCACTTGGCGGCATTTTATAATCGTGCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTGAGAAAAAAGGTGCCGCAGCACCTTTTTAATATAGAGGTTTTGTTGCCACACAGCAGCCAGCAGCGt                                                <  1:385600/251‑1 (MQ=255)                                                                                                                          aGCATGCGCAACGAGAATACGGTCCCTAAACCCCAGGCCGCGCGCCTCTCACTTGCCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAACCGAATGGGGTAAACATGCGGATTTTTTAGAAAATATTTCGAGAGGGATATGTTTCt                                                                                                                         >  1:260052/1‑176 (MQ=255)                                                                                                                                                  ccTAAACCCCAGGTCGCGCGCCTCTCACTTGGCGTCATTTTATAATCGTTCATTACCCCCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCCAACGAATGGGGGAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAggag                                                                                                      >  1:260760/1‑169 (MQ=255)                                                                                                                                                  ccTAAACCCCAGGTCGCGCGCCTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACcacac                                                               >  1:7000/1‑210 (MQ=255)                                                                                                                                                             ggTCGCGCGCCTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACATCTGTTTTACATTAGCGACTAGTGTAAAGCGGAAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGAGAGTACGGTACAATAAAATCAACGGACatcatc           >  1:411426/1‑251 (MQ=255)                                                                                                                                                                 gcgcgcCTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGCCTAGTGTAAAGCGGCAAACGAATGGGGTAACCATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAg                                                                                                >  1:196641/1‑162 (MQ=255)                                                                                                                                                                 gcgcgcCTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTCGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagcagcc                                                        >  1:63451/1‑202 (MQ=255)                                                                                                                                                                   ncgcCTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTCTACAGCGGCACACGAATGGGGTAAACATGCGGATTTTTTAGTAACTATTTCGAGAGGGa                                                                                                                                  >  1:87536/2‑126 (MQ=255)                                                                                                                                                                    cgcCTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTNNACATGc                                                                                                                                                               >  2:14492/1‑96 (MQ=255)                                                                                                                                                                    cgcCTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATg                                                                                                                              <  1:14492/129‑1 (MQ=255)                                                                                                                                                                      ccTCTCACTTGGCGTCATTTTATAATCGTTCATTACCCCCTCTGTTTTAAATTCGCGAGTAGTGTAAAGCGGCAAACGAAAGGGGTAAACATGCGGCTTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAgg  >  1:413528/1‑251 (MQ=255)                                                                                                                                                                      ccTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCATCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAgg  <  1:145720/251‑1 (MQ=255)                                                                                                                                                                      ccTCTCACTTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAgg  >  1:293427/1‑251 (MQ=255)                                                                                                                                                                              |                                                                                                                                                                                                                                                   AATCTGAAAAACGGCCTGAGTCAGACCATAAATACCAATGGCAATACCGATTAATGCTTCGCTGGCACCTTGCAGAGCCATGCCGTACGTGGTCAGAACCGGCAGAACCATAAACATGCCCAGCATGCGCAACGAGAATACGGTCCCTAAACCCCAGGTCGCGCGCCTCTCACCTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGG  >  NC_000913/446469‑446884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑