Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	426,428	C→T	L98F (CTT→TTT)	tgt →	tRNA‑guanine transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	426,428	0	C	T	100.0%	30.3 / NA	11	L98F (CTT→TTT)	tgt	tRNA‑guanine transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/2);  total (9/2)

CCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGATGAGTGTACGCCGTATCCTGCTGACTGGGATTACGCAAAACGCTCCATGGAGATGTCTCTGCGTTGGGCGAAGCG  >  NC_000913/426184‑426639                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                    ccTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCTTTGGCg                                                                                                                                                                                                               >  1:20455/1‑251 (MQ=255)                                                     aCCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGAGCAAATTATCCTCGGAAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGACATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCTTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACgg                                                                                                                                                           >  1:288869/1‑251 (MQ=255)                                                            gCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCTTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCg                                                                                                                                                    >  1:378380/1‑251 (MQ=255)                                                                              cGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGAGCCCGGGCCAGGAAATCATGACACTGCAAGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGCCTCCGGCGGCTTCCAGGTCTTCAGCTTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGa                                                                                                                                   >  1:384798/1‑250 (MQ=255)                                                                                                          gcAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCACGGGCCAGGCAATCATGAAAATGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGAGCCGATCTTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCTTTGGCGATATTCGTAAAATCACAGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTg                                                                                                      >  1:424091/1‑251 (MQ=255)                                                                                                                                                  ccGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCTGCGGCTTCCAGGTCTTCAGCTTTGGCGATATTCg                                                                                                                                                                                                        <  1:152614/113‑1 (MQ=255)                                                                                                                                                       ccAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCTTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGATGAGTGTACGCCGTATCctg                                                         >  1:101487/1‑251 (MQ=255)                                                                                                                                                                                                              ncGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCTTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGATGAGTGTACGCCGTATCCTGCTGACTGGGATTACGCAAAACGCTCCATGGAGATGTCTCTGCGTTGGGCGAAGCg  >  1:110429/2‑251 (MQ=255)                                                                                                                                                                                                                                 gcggcTTCCAGGTCTGCAGCTTTGGCGATATTCTTAAAAGCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTg                                                                                                                                    <  1:212263/102‑1 (MQ=255)                                                                                                                                                                                                                                    gcTTCCAGGTCTTCAGCTTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGga                                                                                                                       >  1:142922/1‑112 (MQ=255)                                                                                                                                                                                                                                     cTTCCAGGTCTTCAGCTTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCNNCGATCCNNa                                                                                                                                   >  2:14493/1‑99 (MQ=255)                                                                                                                                                                                                                                      ttCCANNTANNNAGNNTTGGCGATATTCGTAAAATCACCNAACAGGGCGTGCACTTCCGTAACCCGATCNANGNCGATNNGATNTTCCTCGATCNTGAAAAATCAATGGAGATTGAGTACGATCTTGGTTCGGATATCGACATNANCTTTGATGCGTATACGCCTTATCCTGCTGACNGGGATTACGCAAAACGCTCCATGgngctgnnccngn               >  2:338215/1‑204 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                    CCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGATGAGTGTACGCCGTATCCTGCTGACTGGGATTACGCAAAACGCTCCATGGAGATGTCTCTGCGTTGGGCGAAGCG  >  NC_000913/426184‑426639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑