Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,615,345	C→T	G67S (GGC→AGC)	yjjW ←	putative glycyl‑radical enzyme activating enzyme YjjW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,615,345	0	C	T	100.0%	48.1 / NA	17	G67S (GGC→AGC)	yjjW	putative glycyl‑radical enzyme activating enzyme YjjW
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/8);  total (9/8)

GATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGTTCC  >  NC_000913/4615108‑4615569                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                 gaTGGCGCAGTGGCGGATCGTTTTTGATAGCAGTAAACAGCGCCCCCACAAACGGCGGCTGGGTCGTGGTTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCATGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGc                                                                                                                                                                                                                     <  1:430460/251‑1 (MQ=255)                        ttGATAGCAGAAAACAGCGCCACCACAAAGGGCATCTGGGTCGGGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGACTAAGCACTTCGGCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACGTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACtgtg                                                                                                                                                                                               <  1:440748/250‑1 (MQ=255)                                        gcgcCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCcac                                                                                                                                                                                >  1:429180/1‑249 (MQ=255)                                                            tctgTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATTGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCg                                                                                                                                                           <  1:164069/247‑1 (MQ=255)                                                                                       nttATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCTgcg                                                                                                                               >  1:113632/2‑251 (MQ=255)                                                                                                      aaCAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGAGCGTGGCATGTTGCGGACACCTCGTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGGCATTGCAACGGCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTg                                                                                                                <  1:202766/251‑1 (MQ=255)                                                                                                               gCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCCGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGCTTGTGACAGTTTTTGCAGCGCAGCTTGCAGCCCTGCAAAAACa                                                                                                        >  1:57983/1‑250 (MQ=255)                                                                                                                              cTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGCCGTGGCATGTTGCGGACACCTCTTCAGGCAGGCATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGcc                                                                                        >  1:222977/1‑251 (MQ=255)                                                                                                                                  gCACTTCGTCCACGCTCATGGATTGCGCCATGGGAGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGc                                                                                    <  1:20806/251‑1 (MQ=255)                                                                                                                                   cACTTCGTCCACGCTCATGGCTTGCGCCATGGGAGTGGCATGTTGCGGACCCCTCTTCAGGCAGGTATCACCCTGCTCGCAAAACACCGCGTTCTACACCACTTTGATGTCAACAATCTGCAACGCCTGATGCGGTCACTGTGGCACGCACTCCCCACAGTCATTGc                                                                                                                                                                       >  1:399621/1‑167 (MQ=255)                                                                                                                                                    tGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAgcg                                                                                                                               <  1:330566/190‑1 (MQ=255)                                                                                                                                                                                  gACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGgc                                                                                                                                                                                             >  1:351935/1‑98 (MQ=255)                                                                                                                                                                                                     aTCACACTGCTCGCAAACCACAGCGATCAACACCACTTTACTGTCAACAATCCGCCACGCACGGCCCCGCCACTGCCCCACGCACTCCCAACCGTCATTGCAACGTCCCATCGTCCTCGGATTCAGAAAGTTTTTGCAGCGCAGATTGTCGCCCTGCaaccac                                                                                                         >  1:447739/1‑159 (MQ=255)                                                                                                                                                                                                        acacTGCTCGCCAACCAAAGCGTTCCACACCAATTTGCTGTCAACAATCTGCCACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCACCGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAACAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCAcgcgc              >  1:146378/1‑251 (MQ=255)                                                                                                                                                                                                                cGCAAACCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTgn        <  1:116578/249‑2 (MQ=255)                                                                                                                                                                                                                   aaaCCACAGCGTTCCACACCACTTTGCTGTCAACAAGCTGCAACGTCTGATGCGGACACTGTGGCACGCGCTCCCCGCAGTCATTGCAACGTCCTATCGTTCACGGATTGTGACAGTTTTTGCAGTGCAGATTGCAGCCCTGCAAAAACAGATCCAGACGACTGCCTGGCCCTTCAGCGCAAGAGAAGAGTAGAATTTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTAGCACGCGCGGCTGGCGTTc   <  1:421288/251‑1 (MQ=255)                                                                                                                                                                                                                     aCCACAGCGTTCCACACCACTTTGCTGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGTTcc  >  1:362571/1‑250 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                 GATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTCCACGCTCATGGATTGCGCCATGGGCGTGGCATGTTGCGGACACCTCTTCAGGCAGGTATCACACTGCTCGCAAACCACAGCGTTCCACACCACTTTGCCGTCAACAATCTGCAACGCCTGATGCGGACACTGTGGCACGCACTCCCCACAGTCATTGCAACGTCCCATCGTCCACGGATTGTGACAGTTTTTGCAGCGCAGATTGCAGCCCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGCGTTCC  >  NC_000913/4615108‑4615569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑