Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,604,109	G→T	A211A (GCG→GCT)	yjjQ →	DNA‑binding transcriptional repressor YjjQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,604,109	0	G	T	100.0%	28.1 / NA	12	A211A (GCG→GCT)	yjjQ	DNA‑binding transcriptional repressor YjjQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/6);  total (6/6)

GACCTCCCCGGCAATGACTGAAACTCATGATTTTAGCTATCGCTCCGTCATTCTCACTCTTTCAGAGCGCAAGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCAAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCA  >  NC_000913/4603884‑4604244                                                                                                                                                                                                                                  |                                                                                                                                        gACCTCCCCGGCAATGACTGAAACTCATTATTTTAGCTATCGCTCCGTCATTCTCACTCTTTCAGAGCGCAAGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTGAAGAAAAGTAATAAAACTATCAGCGCCCAAAAAAACAGTGCGATGTGTCGACTGGCAATTCACAGCAACTCGGAAATGTATGCATGGATAAATAGCGCTCAGGGTGCAAGAGAACTTAACTTg                                                                                                                 <  1:301789/250‑1 (MQ=255)                             gATTTTAGCTATCGCTCCGTCATTCTCACTCTTTCAGAGCGCAAGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCAAAAAAACAGTGCGATGCGTTGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCTCAGGGTGCAAGAGAACTTAACGTGCCTTCTGTTTATGGAGATGCCGCAGAATg                                                                                    <  1:142600/251‑1 (MQ=255)                                                        aCTCTTTCAGAGCGCAAGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCAAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCTCAGGGTGCAAGAGAACTTAACTTGc                                                                                                                <  1:31427/196‑1 (MQ=255)                                                                     ncAAGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCAAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCTCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATgg                                                                                   >  1:271030/2‑212 (MQ=255)                                                                               gCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCAAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCTCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATa                                             >  1:231071/1‑240 (MQ=255)                                                                                                                             cTTAAGAAAAGTAATAAAACTATCAGCGCCCAAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGAATGCATGGATAAATAGCGCTCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGc                                                                                             >  1:402944/1‑146 (MQ=255)                                                                                                                                   nnnnnnaannnNNCTANCAGCGCCCAAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCTCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATgg                                                                                   <  2:271030/144‑1 (MQ=255)                                                                                                                                      aGTAATAAAACTATCAGCGCCCAAAAAAACAGTGCGATGCGTCGACTGGCAATTCACGGCAACGCTGAAATGTATGCATGGATAAATAGCGCTCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATg                                                        >  1:605/1‑174 (MQ=255)                                                                                                                                                cTATCAGCGCCCAAAAAAACAGTGCGATGCGTCGACTGGCAATTCACCGCAACGCTGTAATGTATGCATGGATAAATGGCGCTCAGGGTGCAAGAGCACTTAACTTGCGTTCTCTTTATGAAGATGCCGCAGACTGGAACACAGCCGAATTaa                                                                   >  1:219279/1‑153 (MQ=255)                                                                                                                                                     aGCGCCCAAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCTCAGGGTGCAAGAGAACTTAACTTGCCTTCTGttt                                                                                                       >  1:29266/1‑112 (MQ=255)                                                                                                                                                                 aaCAGTGCGATGCGTCGACTTGCAATGCGCAGCAACGCTGAAATGTATGCATGGATAAATAGCGCTCAGGGTGCAAGAGAACTTAACTTTCCGTCTTTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGTGAAATGTCGCACTCATAGATAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCa  <  1:76455/201‑1 (MQ=255)                                                                                                                                                                                                                 gNNTGGATANNTAGCGCTCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATg                                                        <  2:605/99‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                        GACCTCCCCGGCAATGACTGAAACTCATGATTTTAGCTATCGCTCCGTCATTCTCACTCTTTCAGAGCGCAAGGTACTGCGGCTATTAGGTAAAGGATGGGGCATCAACCAGATAGCTTCATTGCTTAAGAAAAGTAATAAAACTATCAGCGCCCAAAAAAACAGTGCGATGCGTCGACTGGCAATTCACAGCAACGCTGAAATGTATGCATGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCA  >  NC_000913/4603884‑4604244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑